I-Enterovirus Universal, EV71 kanye ne-CoxA16 Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-EV010-Enterovirus Universal, EV71 kanye ne-CoxA16 (i-Fluorescence PCR)
I-Epidemiology
Isifo somlomo wesandla siyisifo esithathelwanayo esibangelwa ama-enterovirus. Njengamanje, kutholakale ama-serotypes angu-108 ama-enterovirus, ahlukaniswe ngamaqembu amane: A, B, C kanye no-D. Phakathi kwawo, i-enterovirus EV71 kanye ne-CoxA16 yizona zifo eziyinhloko. Lesi sifo sivame ukwenzeka ezinganeni ezingaphansi kweminyaka emi-5 ubudala, futhi singadala i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye, kanti inani elincane lezingane lingabangela izinkinga ezifana ne-myocarditis, i-pulmonary edema, i-aseptic meningoencephalitis, njll.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | Oama-ropharyngeal swabs,Hamasampula oketshezi lwe-erpes |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhishwa kufanele kwenziwe ngokwemiyalelo. Umthamo wesampula okhishwe ungama-200μL, kanti umthamo wokususa onconywayo ungama-80μL.
