I-Enterovirus Universal
Igama lomkhiqizo
I-HWTS-EV001- I-Entovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Isifo sesandla sezandla-izinyawo-siyisifo esithathelwanayo esibangelwa ama-etoloviruse (eV). Njengamanje, izinhlobo eziyi-108 zama-serotypes zama-etoloviruses atholakele, ahlukaniswe ngamaqembu amane: a, B, C no-D. Lesi sifo sivela kakhulu ezinganeni ezingaphansi kweminyaka engu-5, futhi singadala ama-herpes ezandleni, izinyawo, umlomo kanye nezinye izingxenye. Inani elincane lezingane lizothuthukisa izinkinga ezifana neMyOCarditis, i-Pulmonary Edema, ne-Aseptic Meningoencephalitis.
Insungubezi
| -Yenza | Ev rna |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | I-Oropharyngengeal Swap,Uketshezi lwe-herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | 500copies / ml |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystem 7500/7500 ngokushesha amasistimu we-PRCR yesikhathi sangempela, I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
Ncoma ikhithi yokukhishwa I-HWTS-3006B, i-HWTS-3006C), kufanele ikhishwe ngokuqinile ngokuya ngemiyalo. I-tamputha yesampula ingu-200 μl, ivolumu yokuqedwa enconyiwe liyi-80μL.
Inketho 2.
Ikhithi yokukhishwa enconyiwe: I-Macro & Micro-Test Samputer Reled Reled Reagent (HWTS-3005-8), kufanele ikhishwe ngokuqondile ngokwemiyalo.
Inketho3.
Ikhithi yokukhipha inconyiwe I-tamputha yesampula ingu-140 μL, ivolumu yokuqedwa enconyiwe ingu-60μL.
