I-Enterovirus Universal
Igama lomkhiqizo
I-HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hand-foot-mouth isifo isifo esithathelwanayo esibangelwa ama-enteroviruses (EV).Njengamanje, kutholakale izinhlobo ezingu-108 ze-serotypes ze-enteroviruses, ezihlukaniswe ngamaqembu amane: A, B, C no-D. Phakathi kwabo, i-enterovirus EV71 ne-CoxA16 yizifo eziyinhloko.Lesi sifo sivame ukuvela ezinganeni ezingaphansi kweminyaka engu-5 ubudala, futhi zingabangela i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye.Inombolo encane yezingane izoba nezinkinga ezifana ne-myocarditis, i-pulmonary edema, ne-aseptic meningoencephalitis.
Isiteshi
| FAM | EV RNA |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab,I-Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Amakhophi/mL |
| Izinsimbi Ezisebenzayo | Applied Biosystems 7500/7500 Fast Real-Time PCR Systems, I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Ncoma Ikhithi Yokukhipha: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), kufanele ikhishwe ngokulandela imiyalelo.Ivolumu yesampula ingu-200 μL, ivolumu ye-lution enconyiwe ingu-80µL.
Inketho yesi-2.
Ikhithi yokukhipha enconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), kufanele ikhishwe ngokulandela imiyalelo.
Inketho3.
Ikhithi yokukhipha enconyiwe: I-QIAamp Viral RNA Mini Kit (52904) noma i-Nucleic Acid Extraction noma i-Purification Kit (YDP315-R), kufanele ikhishwe ngokuhambisana nemiyalelo.Ivolumu yesampula ingu-140 μL, ivolumu ye-lution enconyiwe ingu-60µL.
