I-Enterovirus Universal
Igama lomkhiqizo
I-HWTS-EV001- Ikhithi Yokuthola I-Enterovirus Universal Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Isifo somlomo wesandla siyisifo esithathelwanayo esibangelwa ama-enterovirus (EV). Njengamanje, kutholakale izinhlobo ezingu-108 zama-serotypes ama-enterovirus, ahlukaniswe ngamaqembu amane: A, B, C kanye no-D. Phakathi kwawo, i-enterovirus EV71 kanye ne-CoxA16 yizona zifo eziyinhloko. Lesi sifo sivame ukwenzeka ezinganeni ezingaphansi kweminyaka emi-5 ubudala, futhi singadala i-herpes ezandleni, ezinyaweni, emlonyeni nakwezinye izingxenye. Inani elincane lezingane lizoba nezinkinga ezifana ne-myocarditis, i-pulmonary edema, kanye ne-aseptic meningoencephalitis.
Isiteshi
| I-FAM | I-EV RNA |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab,Uketshezi lwe-Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-Biosystems ezisetshenziswayo 7500/7500 ze-PCR ezisheshayo zesikhathi sangempela, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
Ikhithi Yokukhipha Enconyiwe: Ikhithi Ye-Macro & Micro-Test General DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), kufanele ikhishwe ngokuqinile ngokwemiyalelo. Ivolumu yesampula ingu-200 μL, ivolumu yokususa enconyiwe ingu-80µL.
Inketho 2.
Ikhithi yokukhipha enconywayo: I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), kufanele ikhishwe ngokuqinile ngokwemiyalelo.
Inketho 3.
Ikhithi yokukhipha enconyiwe: I-QIAamp Viral RNA Mini Kit (52904) noma i-Nucleic Acid Extraction noma i-Purification Kit (YDP315-R), kufanele ikhishwe ngokuhambisana nemiyalelo. Umthamo wesampula ungu-140 μL, umthamo wokususa onconyiwe ungu-60µL.
