Izinhlobo eziyi-11 eziqandisiwe zama-Pathogens Okuphefumula I-Nucleic Acid
Igama lomkhiqizo
I-HWTS-RT190 -Omisiwe-Omisiwe-Omisiwe Izinhlobo eziyi-11 Zamagciwane Aphefumulayo I-Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
Ukutheleleka komgudu wokuphefumula kuyisifo esibalulekile esisongela kakhulu impilo yabantu. Ucwaningo luye lwabonisa ukuthi izifo eziningi zepheshana lokuphefumula zibangelwa amagciwane kanye/noma amagciwane ahlasela umsingathi, okuholela ekwandeni kobunzima besifo noma ngisho nokufa. Ngakho-ke, ukuhlonza i-pathogen kunganikeza ukwelashwa okuhlosiwe futhi kuthuthukise izinga lokusinda kwesiguli[1,2]. Kodwa-ke, izindlela zendabuko zokuthola amagciwane okuphefumula zifaka ukuhlolwa okuncane, isiko lebhaktheriya, nokuhlolwa kwe-immunological. Lezi zindlela ziyinkimbinkimbi, zidla isikhathi, zidinga ubuchwepheshe, futhi zinokuzwela okuphansi. Ngaphezu kwalokho, abakwazi ukubona izifo eziningi ze-pathogen kusampula eyodwa, okwenza kube nzima ukunikeza odokotela ukuxilongwa okunembile okuwusizo. Ngenxa yalokho, imithi eminingi isesigabeni semithi yokwelapha, engasheshi nje kuphela umjikelezo wokumelana nebhaktheriya, kodwa futhi ithinta ukutholakala kweziguli ngesikhathi[3]. I-Common Haemophilus influenzae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa, i-Klebsiella pneumoniae, i-Streptococcus pneumoniae, i-Stenotrophomonas maltophilia, i-Bordetella pertussis, i-Bordetella parapertussis, i-Mycoplasma pneumoniae, i-Chlamydia pneumonia ebaluleke kakhulu ebangelwa i-Chlamydia pneumonia ye-Leethogen izifo ze-nosocomial zokuphefumula [4,5]. Le khithi yokuhlola ithola futhi ihlonze ama-nucleic acid athile amagciwane angenhla kubantu abanezimpawu nezimpawu zokutheleleka kokuphefumula, futhi iyihlanganise neminye imiphumela yaselabhorethri ukuze isize ekuxilongweni kwegciwane le-pathogen yokuphefumula.
Imingcele Yezobuchwepheshe
| Isitoreji | 2-30 ℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | I-LoD yekhithi ye-Klebsiella pneumoniae yi-500 CFU/mL; i-LoD ye-Streptococcus pneumoniae yi-500 CFU/mL; i-LoD ye-Haemophilus influenzae yi-1000 CFU/mL; i-LoD ye-Pseudomonas aeruginosa ingama-500 CFU/mL; i-LoD ye-Acinetobacter baumannii ingama-500 CFU/mL; i-LoD ye-Stenotrophomonas maltophilia yi-1000 CFU/mL; i-LoD ye-Bordetella pertussis yi-500 CFU/mL; i-LoD ye-Bordetella parapertussis yi-500 CFU/mL; i-LoD ye-Mycoplasma pneumoniae ingamakhophi angu-200/mL; i-LoD ye-Legionella pneumophila ingu-1000 CFU/mL; I-LOD ye-Chlamydia pneumoniae ingamakhophi angu-200/mL. |
| Ukucaciswa | Akukho ukusabela okuphambanayo phakathi kwekhithi namanye amagciwane avamile okuphefumula ngaphandle kwebanga lokutholwa kwekhithi yokuhlola, isb. Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoccuss, Micrococcus, Stregene, I-Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Igciwane le-Influesperanza, Influesperanza I-Aspergillus terreus, i-Aspergillus fumigatus, i-Candida glabrata, ne-Candida tropicalis. |
| Izinsimbi Ezisebenzayo | Uhlobo I: Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 I-Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad PCR Real System OTime9 Uhlobo II: EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Ukugeleza komsebenzi
Uhlobo I: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Test Med-Tech Co. ngokuhambisana ne-IFU yekhithi.
