Izinhlobo eziyi-11 zezifo zokuphefumula ezomisiwe ngeqhwa I-Nucleic Acid
Igama lomkhiqizo
I-HWTS-RT190 -Yomiswe ngeqhwa-yomisiwe-yomisiwe-yomisiwe Izinhlobo eziyi-11 ze-Respiratory Pathogens Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Ukutheleleka komgudu wokuphefumula kuyisifo esibalulekile esisongela impilo yabantu kakhulu. Ucwaningo luye lwabonisa ukuthi izifo eziningi zomgudu wokuphefumula zibangelwa amagciwane amagciwane kanye/noma amagciwane athelela ndawonye umninimzi, okuholela ekwandeni kobunzima besifo noma ngisho nokufa. Ngakho-ke, ukuhlonza igciwane kunganikeza ukwelashwa okuqondiwe futhi kuthuthukise izinga lokusinda kwesiguli[1,2]. Kodwa-ke, izindlela zendabuko zokuthola amagciwane okuphefumula zifaka phakathi ukuhlolwa okuncane, ukukhuliswa kwamagciwane, kanye nokuhlolwa kwe-immunological. Lezi zindlela ziyinkimbinkimbi, zidla isikhathi, zidinga ubuchwepheshe, futhi zinokuzwela okuphansi. Ngaphezu kwalokho, azikwazi ukuthola amagciwane amaningi kusampula eyodwa, okwenza kube nzima ukunikeza odokotela ukuxilongwa okunembile okusizayo. Ngenxa yalokho, imithi eminingi isesesigabeni semithi esetshenziswayo, engasheshisi nje kuphela umjikelezo wokumelana namagciwane, kodwa futhi ithinta ukuxilongwa kweziguli ngesikhathi esifanele[3]. I-Common Haemophilus influenzae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa, i-Klebsiella pneumoniae, i-Streptococcus pneumoniae, i-Stenotrophomonas maltophilia, i-Bordetella pertussis, i-Bordetella parapertussis, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, kanye ne-Legionella pneumophila yizifo ezibalulekile ezibangela ukutheleleka kwe-nosocomial respiratory tract [4,5]. Le khithi yokuhlola ithola futhi ihlonze ama-nucleic acid athile ezifo ezingenhla kubantu abanezimpawu zokutheleleka kokuphefumula, futhi iyihlanganisa neminye imiphumela yelabhorethri ukusiza ekuxilongweni kokutheleleka kwezifo zokuphefumula.
Amapharamitha Obuchwepheshe
| Isitoreji | 2-30℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| Ct | ≤33 |
| CV | <5.0% |
| I-LoD | I-LoD yekhithi ye-Klebsiella pneumoniae ingu-500 CFU/mL; i-LoD ye-Streptococcus pneumoniae ingu-500 CFU/mL; i-LoD ye-Haemophilus influenzae ingu-1000 CFU/mL; i-LoD ye-Pseudomonas aeruginosa ingu-500 CFU/mL; i-LoD ye-Acinetobacter baumannii ingu-500 CFU/mL; i-LoD ye-Stenotrophomonas maltophilia ingu-1000 CFU/mL; i-LoD ye-Bordetella pertussis ingu-500 CFU/mL; i-LoD ye-Bordetella parapertussis ingu-500 CFU/mL; i-LoD ye-Mycoplasma pneumoniae ingu-200 amakhophi/mL; i-LoD ye-Legionella pneumophila ingu-1000 CFU/mL; I-LoD ye-Chlamydia pneumoniae ingamakhophi angu-200/mL. |
| Ukucaciswa | Akukho ukusabela okuhlangene phakathi kwekhithi nezinye izifo ezivamile zokuphefumula ngaphandle kobubanzi bokutholwa kwekhithi yokuhlola, isib. I-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Escherichia coli, i-Serratia marcescens, i-Enterococcus faecalis, i-Candida albicans, i-Klebsiella oxytoca, i-Streptococcus pyogenes, i-Micrococcus luteus, i-Rhodococcus equi, i-Listeria monocytogenes, i-Acinetobacter junii, i-Haemophilus parainfluenzae, i-Legionella dumov, i-Enterobacter aerogenes, i-Haemophilus haemolyticus, i-Streptococcus salivarius, i-Neisseria meningitidis, i-Mycobacterium tuberculosis, i-Influenza A virus, i-Influenza B virus, i-Aspergillus flavus, i-Aspergillus terreus, i-Aspergillus fumigatus, i-Candida glabrata, kanye ne-Candida tropicalis. |
| Izinsimbi Ezisebenzayo | Uhlobo I: Izinhlelo ze-Biosystems ezisetshenzisiwe 7500 Real-Time PCR, Izinhlelo ze-Biosystems ezisetshenzisiwe 7500 Fast Real-Time PCR, i-QuantStudio®Izinhlelo ezi-5 ze-PCR Zesikhathi Sangempela, Izinhlelo ze-SLAN-96P ze-PCR Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), i-LightCycler®Izinhlelo ze-PCR zesikhathi sangempela ezingu-480, Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), i-BioRad CFX96 Real-Time PCR System, i-BioRad CFX Opus 96 Real-Time PCR System. Uhlobo II: EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Ukugeleza Komsebenzi
Uhlobo I: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. iyanconywa ekukhishweni kwesampula futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana ne-IFU ye-Kit.
