Igciwane Lomkhuhlane/Igciwane Lomkhuhlane B elifakwe efrijini i-Nucleic Acid

Igciwane/Igciwane Lomkhuhlane B Eliqandisiwe Isithombe Esifakiwe se-Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola igciwane le-in vitro A (IFV A) kanye negciwane le-influenza B (IFV B) RNA kumasampula e-nasopharyngeal swab yomuntu.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola I-Nucleic Acid Yomkhuhlane Eyomisiwe Nge-HWTS-RT193/Igciwane Le-Influenza B (i-Fluorescence PCR)

I-Epidemiology

Ngokusho komehluko we-antigenic phakathi kwezakhi zofuzo ze-NP kanye ne-M, amagciwane omkhuhlane angahlukaniswa ngezinhlobo ezine: igciwane le-influenza A (IFV A), igciwane le-influenza B (IFV B), igciwane le-influenza C (IFV C) kanye negciwane le-influenza D (IFV D). Ngegciwane le-influenza A, linabasingathi abaningi kanye nama-serotype ayinkimbinkimbi, futhi lingasakazeka kubo bonke abasingathi ngokusebenzisa ukuhlanganiswa kwezakhi zofuzo kanye nokuguquguquka okuguquguqukayo. Abantu abanakho ukuzivikela okuhlala isikhathi eside egciwaneni le-influenza A, ngakho-ke abantu babo bonke ubudala ngokuvamile basengozini. Igciwane le-Influenza A liyigciwane elikhulu kakhulu elibangela ubhubhane lwe-influenza. Ngegciwane le-influenza B, lisakazeka kakhulu ezindaweni ezincane futhi okwamanje alinazo izinhlobo ezincane. Ukutheleleka kwabantu kubangelwa kakhulu amagciwane omkhuhlane we-B/Yamagata noma i-B/Victoria lineages. Phakathi kwamacala aqinisekisiwe nyanga zonke e-influenza emazweni angu-15 esifundeni sase-Asia-Pacific, izinga lokutholakala kwegciwane le-influenza B lisukela ku-0 kuya ku-92%. Ngokungafani negciwane le-influenza A, amaqembu athile abantu, njengezingane kanye nabantu asebekhulile, asengozini yegciwane le-influenza B, okungadala izinkinga kalula, okuletha umthwalo omkhulu emphakathini kunegciwane le-influenza A.

Amapharamitha Obuchwepheshe

Isitoreji	2-28℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-swab yomphimbo
Ct	I-IFV A，I-IFVB Ct≤35
CV	<5.0%
I-LoD	Amakhophi angu-200/mL
Ukucaciswa	Ukusabela okuxubile: Akukho ukusabela okuxubile phakathi kwekhithi ne-Bocavirus, i-Rhinovirus, i-Cytomegalovirus, igciwane lokuphefumula elihambisanayo, igciwane le-Parainfluenza, igciwane le-Epstein-Barr, igciwane le-herpes simplex, igciwane le-varicella-zoster, igciwane le-Mumps, igciwane le-Enterovirus, igciwane le-Measles, igciwane le-human metapneumovirus, i-Adenovirus, i-human coronaviruses, i-novel coronavirus, i-SARS-CoV, i-MERS-CoV, i-Rotavirus, i-Norovirus, i-Chlamydia pneumoniae, i-Mycoplasma pneumoniae, i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Streptococcus pyogenes, i-Legionella, i-Pneumocystis jirovecii, i-Haemophilus influenzae, i-Bordetella pertussis, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-Neisseria gonorrhoeae, i-Candida albicans, i-Candida glabrata, i-Aspergillus fumigatus, i-Cryptococcus neoformans, i-Streptococcus salivarius, i-Moraxella ama-catarrhalis, i-Lactobacillus, i-Corynebacterium kanye ne-DNA ye-genomic yomuntu. Ukuhlolwa kokuphazamiseka: I-Mucin (60mg/mL), igazi lomuntu (50%), i-Phenylephrine (2 mg/mL), i-Oxymetazoline (2mg/mL), i-Sodium chloride (20mg/mL) ene-5% yokuvikela, i-Beclomethasone (20mg/mL), i-Dexamethasone (20mg/mL), i-Flunisolide (20μg/mL), i-Triamcinolone (2mg/mL), i-Budesonide (1mg/mL), i-Mometasone (2mg/mL), i-Fluticasone (2mg/mL), i-Histamine hydrochloride (5 mg/mL), i-Benzocaine (10%), i-Menthol (10%), i-Zanamivir (20mg/mL), i-Peramivir (1mg/mL), i-Mupirocin (20mg/mL), i-Tobramycin (0.6mg/mL), i-Oseltamivir (60ng/mL), i-Ribavirin (10mg/L) yakhethwa ngenxa yokuphazamiseka. ukuhlolwa, futhi imiphumela ibonise ukuthi izinto eziphazamisayo ekugxilweni okungenhla azizange ziphazamiseke emiphumeleni yokuhlolwa kwekhithi.
Izinsimbi Ezisebenzayo	Kusebenza ku-reagent yokuhlola yohlobo lwe-I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.). Kusebenza ku-reagent yokuhlola yohlobo lwesibili: I-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Ukugeleza Komsebenzi

I-PCR evamile

I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. iyanconywa ekukhishweni kwesampula futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana ne-IFU ye-Kit.

Umshini we-AIO800 ohlanganisa konke