Ikhithi yokuthola igciwane le-Herpes simplex uhlobo 1/2, (HSV1/2) nucleic acid
Igama lomkhiqizo
Ikhithi yokuthola igciwane le-Herpes simplex uhlobo 1/2, (HSV1/2) nucleic acid
I-Epidemiology
Izifo ezithathelwana ngocansi (STD) ziseseyinye yezinsongo ezinkulu ekuphepheni kwempilo yomphakathi emhlabeni jikelele. Izifo ezinjalo zingaholela ekungazalini, ekuzalweni komntwana ngaphambi kwesikhathi, ekuvuvukeni kanye nezinkinga eziningi ezinkulu. Kunezinhlobo eziningi zezifo ezithathelwana ngocansi, okuhlanganisa amagciwane, amagciwane, i-chlamydia, i-mycoplasma kanye nama-spirochetes, phakathi kwazo i-Neisseria gonorrhoeae, i-Mycoplasma genitalium, i-Chlamydia trachomatis, i-HSV1, i-HSV2, i-Mycoplasma hominis, kanye ne-Ureaplasma urealyticum ezivamile.
I-genital herpes yisifo esivamile esidluliselwa ngocansi esibangelwa yi-HSV2, esithathelwana kakhulu. Eminyakeni yamuva nje, ukwanda kwe-genital herpes kukhuphuke kakhulu, futhi ngenxa yokwanda kwemikhuba yocansi eyingozi, izinga lokutholakala kwe-HSV1 ku-genital herpes likhuphuke futhi kwabikwa ukuthi liphezulu ngamaphesenti angama-20-30%. Ukutheleleka kokuqala ngegciwane le-genital herpes ngokuvamile akubonakali ngaphandle kwezimpawu zomtholampilo ezisobala ngaphandle kwe-herpes yendawo esikhumbeni noma esikhumbeni seziguli ezimbalwa. Njengoba i-genital herpes ibonakala ngokukhishwa kwegciwane impilo yonke kanye nokuthambekela kokubuyela emuva, kubalulekile ukuhlola amagciwane ngokushesha okukhulu futhi kuvimbele ukudluliselwa kwawo.
Isiteshi
| I-FAM | I-HSV1 |
| CY5 | I-HSV2 |
| I-VIC(HEX) | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | ukukhishelwa komchamo, ukukhishelwa komlomo wesibeletho |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-50/ukusabela |
| Ukucaciswa | Akukho ukuhlangana kabusha namanye amagciwane e-STD afana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Mycoplasma genitalium, ne-Ureaplasma urealyticum. |
| Izinsimbi Ezisebenzayo | Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukuhamba komsebenzi
Kudingeka ama-reagent kodwa awanikezwanga
I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). I-Nucleic Acid Extraction noma i-Purification Kit (YDP315) evela ku-Tiangen Biotech (Beijing) Co., Ltd.
Izinto ezidingekayo kodwa ezinganikezwanga
Amashubhu e-centrifuge angenayo i-DNase/RNase angu-1.5 mL, amathiphu angenayo i-DNase/RNase, i-ethanol ephelele, i-pipettor, i-benchtop centrifuge, i-benchtop vortex mixer.













