I-Hantaan Virus Nucleic
Igama lomkhiqizo
Ikhithi Yokuthola I-Hantaan Virus Nucleic Acid (i-Fluorescence PCR) ye-HWTS-FE005
I-Epidemiology
I-Hantavirus uhlobo lwegciwane le-RNA elimboziwe, elihlukaniswe, nelingenazo izintambo. I-Hantavirus ihlukaniswe ngezinhlobo ezimbili: eyodwa ibangela i-Hantavirus pulmonary syndrome (HPS), kanti enye ibangela i-Hantavirus hemorrhagic fever with renal syndrome (HFRS). Eyokuqala ivame kakhulu eYurophu nase-United States, kanti eyesibili iwumkhuhlane we-hemorrhagic with renal syndrome obangelwa yigciwane le-Hantaan, elivame eShayina. Izimpawu zohlobo lwe-hantavirus hantaan zibonakala kakhulu njengomkhuhlane we-hemorrhagic with renal syndrome, obonakala ngomkhuhlane ophezulu, umfutho wegazi ophansi, ukopha, i-oliguria noma i-polyuria kanye nokunye ukukhubazeka kokusebenza kwezinso. Ibangela izifo kubantu futhi kufanele inakwe ngokwanele.
Isiteshi
| I-FAM | uhlobo lwe-hantavirus hantaan |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Ct | ≤38 |
| CV | ＜5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhipha kufanele kwenziwe ngokwe-IFU. Umthamo wesampula yokukhipha ungu-200μL. Umthamo wokususa onconyiwe ungu-80μL.
Ama-reagent okukhipha anconyiwe: I-Nucleic Acid Extraction noma i-Purification Kit (YDP315-R). Ukukhipha kufanele kwenziwe ngokwe-IFU. Umthamo wesampula yokukhipha ungu-140μL. Umthamo wokususa onconyiwe ungu-60μL.
