Hantaan virus nucleic
Igama lomkhiqizo
I-HWTS-FE005 HANTAAN virus nucleic acid detection detection detection
I-Epidemiology
IHantavirus iluhlobo lwembozo embozwe, oluhlukanisiwe, olungemuhle-strand rna. UHantavirus uhlukaniswe ngezinhlobo ezimbili: umuntu obangela i-hantavirus pulmonary syndrome (HPS), kanti enye ibangela umkhuhlane we-hantavirus hemorrhagic nge-renal syndrome (HFRS). Okwedlule kuvame kakhulu eYurophu nase-United States, kanti owokugcina ungumkhuhlane we-hemorrhagic nge-renal syndrome ebangelwa yi-hantaan virus ebangelwa yi-hantaan virus, okuvamile eChina. Izimpawu ze-hantavirus hantaan uhlobo lubonisa ikakhulukazi njenge-hemorrhagic fever nge-renal syndrome, ebonakala nge-high freedrome, ebonakala nge-high fever, hypotension, ukopha, i-oliguria noma i-polyuria nomsebenzi wokukhubazeka kwemisebenzi. Kungama-pathogenic kubantu futhi kufanele akhokhelwe ngokwanele.
Insungubezi
| -Yenza | UHantavirus Hantaan Type |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | i-serum entsha |
| Ct | ≤38 |
| CV | <5.0% |
| Indlu edayisa insimbi | Amakhophi angama-500 / μL |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reagents yokukhishwa enconyiwe: I-Macro & Micro-Tevation Viral DNA / RNA KIT (HWTS-3017) (engasetshenziswa nge-macro & micro-eq Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuya nge-IFU. Ivolumu yesampula yokukhishwa i-200μL. IVolumu Ye-Expeated Enconyiwe ingu-80μL.
I-reagents yokukhishwa ezinconyiwe Ukukhishwa kufanele kwenziwe ngokuya nge-IFU. Ivolumu yesampula yokukhishwa yi-140μL. IVolumu Ye-Expery Enconyiwe ingu-60μL.
