I-Mycoplasma Pneumoniae (MP)
Igama lomkhiqizo
I-HWTS-RT024 Mycoplasma Pneumoniae (MP) I-Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Mycoplasma pneumoniae (MP) iwuhlobo lwe-microorganism encane kakhulu ye-prokaryotic, ephakathi kwebhaktheriya negciwane, enokwakheka kwamaseli kodwa alukho udonga lweseli.I-MP ikakhulukazi ibangela ukutheleleka komgudu wokuphefumula womuntu, ikakhulukazi ezinganeni nasebancane.Kungabangela inyumoniya ye-mycoplasma yomuntu, ukutheleleka komgudu wokuphefumula wezingane kanye nenyumoniya engavamile.Ukubonakaliswa komtholampilo kuhlukahlukene, iningi lazo ukukhwehlela okukhulu, umkhuhlane, ukugodola, ikhanda elibuhlungu, umphimbo obuhlungu.Ukutheleleka komgudu wokuphefumula ophezulu kanye nenyumoniya ye-bronchial yizona ezivame kakhulu.Ezinye iziguli zingakhula kusukela ekuthelelekeni kwemigudu yokuphefumula ephezulu kuye kunyumoniya enzima, ukucindezeleka kokuphefumula okukhulu nokufa kungase kwenzeke.
Isiteshi
| FAM | I-Mycoplasma pneumoniae |
| VIC/HEX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela, i-Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 amakhophi/mL |
| Ukucaciswa | a） Ukuphinda usebenze kabusha: akukho ukuphinda kusebenze okuphambanayo ne-Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacteriuinodocuum aphilamonaella i-baumannii, igciwane le-Influenza A , Igciwane le-Influenza B, i-Parainfluenza virus uhlobo I/II/III/IV, i-Rhinovirus, i-Adenovirus, i-Human metapneumovirus, i-Respiratory syncytial virus kanye ne-human genomic nucleic acid. b）Amandla okunqanda ukuphazamiseka: akukho ukuphazamiseka lapho izinto eziphazamisayo zihlolwa ngokugxila okulandelayo: i-hemoglobin (50mg/L), i-bilirubin (20mg/dL), i-mucin (60mg/mL), 10% (v/v) igazi lomuntu, i-levofloxacin (10μg/mL), i-moxifloxacin (0.1g/L), i-gemifloxacin (80μg/mL), i-azithromycin (1mg/mL), i-clarithromycin (125μg/mL), i-erythromycin (0.5g/L), i-doxycycline (50mg) /L), i-minocycline (0.1g/L). |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
(1) Isampula yesikhwehlela
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-200µL wosawoti ojwayelekile emvuleni ecutshunguliwe.Ukukhipha okulandelayo kufanele kwenziwe ngokulandela imiyalelo yokusetshenziswa.Ivolumu ye-elution enconyiwe ngu-80µL.Isisetshenziswa esisetshenziswayo esinconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R).Ukukhipha kufanele kwenziwe ngokuqinile ngokuvumelana nomyalelo wokusetshenziswa.Ivolumu ye-elution enconyiwe ngu-60µL.
(2) I-Oropharyngeal swab
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuvumelana nomyalelo wokusetshenziswa.Ivolumu yokukhipha enconyiwe yesampula ngu-200µL, futhi ivolomu enconyiwe ye-elution ngu-80µL.Isisetshenziswa esisetshenziswayo esinconyiwe: QIAamp Viral RNA Mini Kit (52904) noma i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R).Ukukhipha kufanele kwenziwe ngokuqinile ngokuvumelana nomyalelo wokusetshenziswa.Ivolumu yokukhipha enconyiwe yesampula ngu-140µL, futhi ivolomu ye-elution enconyiwe ngu-60µL.
