I-RNA yegciwane le-Hepatitis B
Igama lomkhiqizo
I-HWTS-HP007 Hepatitis B Virus RNA Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Qukutholwa okungalindelekile kwamazinga e-HBV-RNA ku-serum kungaqapha kangcono izinga le-HBV cccDNA kuma-hepatocytes, futhi ngesikhathi esifanayo kugweme ukubangela umonakalo omkhulu ezigulini.Kubaluleke kakhulu ekuxilongeni okuyisizayo kokutheleleka ngegciwane le-hepatitis B (HBV) kanye nokuqapha ukusebenza kahle nokubikezela ukuhoxiswa kwezidakamizwa kwe-NAs yokwelashwa kwe-antiviral ezigulini ze-CHB.
Isiteshi
| FAM | I-HBV-RNA |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | i-serum |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 100 amakhophi/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kokugxambukela ikhombisa ukuthi akukho miphumela ekunqunyweni kwe-HBV RNA lapho ukugcwala kwe-bilirubin ku-serum ingekho ngaphezu kwe-168.2μmol/mL, ukugcwala kwe-hemoglobin ekhiqizwa yi-hemolysis akukho ngaphezu kwe-130g/L, futhi ukugcwala kwe-lipids egazini akukho ngaphezu kwe-65mmol/mL;akukho miphumela ekunqumeni kwe-HBV RNA lapho inani le-IgG eliphelele ku-serum lingeqi ku-5mg/mL.Imiphumela yokuhlolwa kwe-cross-reactivity ibonisa ukuthi akukho ukusabela okuphambanayo phakathi kwale kit namanye amagciwane noma amagciwane atholwe yi-nucleic acid kumasampula egazi (igciwane le-hepatitis C, i-human cytomegalovirus, i-epstein-barr virus, i-human immunodeficiency virus, igciwane le-hepatitis A, ugcunsula. , uhlobo 6 lwegciwane le-herpes, uhlobo 1 lwegciwane le-herpes simplex, igciwane lomkhuhlane A, i-propionibacterium acnes, i-staphylococcus aureus, i-candida albicans) kanye nezakhi zofuzo zabantu;ngemva kokuba iziguli zithathe i-lamivudine, i-telbivudine, i-adefovir dipivoxil ne-entecavir, lapho umthamo wegciwane lesandulela ngculaza we-hepatitis B emzimbeni ungaphezu kwe-LoD encane yekhithi, ikhithi isengatholwa ngokuphumelelayo. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 Fast Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) |
Ukugeleza komsebenzi
Inketho 1.
I-Viral DNA/RNA Isolation Kit (NA007-2) yi-GenMag Biotechnology Co., Ltd. Izinyathelo ezithile zokukhipha sicela ulandele ngokuqinile imanuwali yeziqondiso.
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokuya ngemanuwali yeziqondiso, ivolumu yesampula ekhishiwe ngu-200μL, futhi ivolumu enconyiwe ye-elution ithi 80μL.
