I-Hepatitis E Virus
Igama lomkhiqizo
I-HWTS-HP006 Hepatitis E Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hepatitis E virus (HEV) igciwane le-RNA elidala izinkinga zempilo emhlabeni wonke.Inobasingathi obanzi futhi inendawo yokuwela izithiyo ze-interspecies.Ingenye ye-zoonotic pathogens ebaluleke kakhulu futhi idala umonakalo omkhulu kubantu nasezilwaneni.I-HEV isakazwa kakhulu ngokudluliselwa kwe-fecal-oral, futhi ingasakazwa ngokuqondile ngama-embryo noma igazi.Phakathi kwazo, emzileni wokudluliselwa kwe-fecal-oral, amanzi angcoliswe yi-HEV nokudla kwasabalala kakhulu, kanti ingozi yokutheleleka nge-HEV kubantu nasezilwaneni iphezulu[1-2].
Isiteshi
| FAM | I-HEV nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/μL |
| Ukucaciswa | I-Hepatitis E virus (HEV) igciwane le-RNA elidala izinkinga zempilo emhlabeni wonke.Inobasingathi obanzi futhi inendawo yokuwela izithiyo ze-interspecies.Ingenye ye-zoonotic pathogens ebaluleke kakhulu futhi idala umonakalo omkhulu kubantu nasezilwaneni.I-HEV isakazwa kakhulu ngokudluliselwa kwe-fecal-oral, futhi ingasakazwa ngokuqondile ngama-embryo noma igazi.Phakathi kwazo, emzileni wokudluliselwa kwe-fecal-oral, amanzi angcoliswe yi-HEV nokudla kwasabalala kakhulu, kanti ingozi yokutheleleka nge-HEV kubantu nasezilwaneni iphezulu[1-2]. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR System I-QuantStudio®5 Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Kufanele ikhishwe ngokulandela imiyalelo.Ivolumu ye-elution enconyiwe ngu-80µL.
Inketho yesi-2
I-TIANamp Virus DNA/RNA Kit (YDP315-R) ekhiqizwe i-Tiangen Biotech (Beijing) Co., Ltd. Kufanele ikhishwe ngokulandela imiyalelo.Ivolumu yesampula ekhishiwe ingu-140μL.Ivolumu ye-elution enconyiwe ngu-60µL.v
