I-hepatitis e virus
Igama lomkhiqizo
I-HWTS-HP006 Hepatitis e Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Hepatitis e Virus (HEV) i-rna virus ebangela izinkinga zempilo yomhlaba. Inobubanzi obuthole obubanzi futhi inempahla yokuwela izithiyo ze-Interplepecies. Ungomunye we-pathogens ebaluleke kakhulu ye-zoonotic futhi ubangele ukulimala okukhulu kubantu nasesilwaneni. UHev udluliselwa ngokukhethekile ngokudluliselwa kwe-fecal-ngomlomo, futhi futhi angadluliselwa ngokuqondile ngemibungu noma ngegazi. Phakathi kwazo, emzileni wokuhambisa owomlomo, amanzi angcoliswe eHev nokudla asakazeka kabanzi, futhi ubungozi bokutheleleka kwe-HEV kubantu nasezilwaneni kuphakeme [1-2].
Insungubezi
| -Yenza | Hev nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | UTroat Swab |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | Amakhophi angama-500 / μL |
| Ukucacisa | I-Hepatitis e Virus (HEV) i-rna virus ebangela izinkinga zempilo yomhlaba. Inobubanzi obuthole obubanzi futhi inempahla yokuwela izithiyo ze-Interplepecies. Ungomunye we-pathogens ebaluleke kakhulu ye-zoonotic futhi ubangele ukulimala okukhulu kubantu nasesilwaneni. UHev udluliselwa ngokukhethekile ngokudluliselwa kwe-fecal-ngomlomo, futhi futhi angadluliselwa ngokuqondile ngemibungu noma ngegazi. Phakathi kwazo, emzileni wokuhambisa owomlomo, amanzi angcoliswe eHev nokudla asakazeka kabanzi, futhi ubungozi bokutheleleka kwe-HEV kubantu nasezilwaneni kuphakeme [1-2]. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR I-BIOSYSTEMS I-BIOSYSTEMS 7500 Fast Real-Time Pcr System I-QuantStudio®5 Systems real-time PCREMS ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1
I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3017-50, HWS-3017-50, HWS-3017-48, HWS-3017-48, i-MACLE & Micro-3006c extractor (HWS-3006C, I-HWTS-3006B). Kufanele ikhishwe ngokuya ngemiyalo. IVolumu Ye-Expeated Enconyiwe ingu-80μL.
Inketho 2
I-Tinamp Virus DNA / RNA KIT (yppp15-R) ekhiqizwe yiTiangen Biotech (Beijing) Co, Ltd kufanele ikhishwe ngokuqinile ngokuya ngemiyalo. Ivolumu yesampula ekhishwe yi-140μL. IVolumu Enconyiwe E-ASTY ingu-60μL.v
