Igciwane le-Hepatitis E
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-HP006 Hepatitis E Virus Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Igciwane le-Hepatitis E (HEV) liyigciwane le-RNA elibangela izinkinga zempilo emhlabeni jikelele. Libanzi kakhulu futhi likwazi ukuwela izithiyo zezinhlobo ezahlukene. Lingenye yezifo ezibangela izifo ezibangelwa yizilwane ezizalwa futhi libangela umonakalo omkhulu kubantu nasezilwaneni. I-HEV idluliselwa kakhulu ngokudluliswa kwendle ngomlomo, futhi ingadluliselwa ngqo ngokusebenzisa ama-embryo noma igazi. Phakathi kwazo, endleleni yokudluliselwa kwendle ngomlomo, amanzi nokudla okungcoliswe yi-HEV kusakazeka kabanzi, futhi ingozi yokutheleleka yi-HEV kubantu nasezilwaneni iphezulu [1-2].
Isiteshi
| I-FAM | I-HEV nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-swab yomphimbo |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Ukucaciswa | Igciwane le-Hepatitis E (HEV) liyigciwane le-RNA elibangela izinkinga zempilo emhlabeni jikelele. Libanzi kakhulu futhi likwazi ukuwela izithiyo zezinhlobo ezahlukene. Lingenye yezifo ezibangela izifo ezibangelwa yizilwane ezizalwa futhi libangela umonakalo omkhulu kubantu nasezilwaneni. I-HEV idluliselwa kakhulu ngokudluliswa kwendle ngomlomo, futhi ingadluliselwa ngqo ngokusebenzisa ama-embryo noma igazi. Phakathi kwazo, endleleni yokudluliselwa kwendle ngomlomo, amanzi nokudla okungcoliswe yi-HEV kusakazeka kabanzi, futhi ingozi yokutheleleka yi-HEV kubantu nasezilwaneni iphezulu [1-2]. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Uhlelo lwe-PCR olusetshenziswayo lwe-Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kufanele ikhishwe ngokwemiyalelo. Umthamo wokuhlanza onconywayo ungu-80µL.
Inketho 2
I-TIANamp Virus DNA/RNA Kit (YDP315-R) eyenziwe yiTiangen Biotech (Beijing) Co., Ltd. Kufanele ikhishwe ngokuqinile ngokwemiyalelo. Umthamo wesampula okhishwe ngu-140μL. Umthamo wokuhlanza onconywayo ngu-60μL.v
