Ubuningi be-HIV
Igama lomkhiqizo
Ikhithi Yokuthola Ubuningi Be-HIV ye-HWTS-OT032 (i-Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Igciwane i-Human Immunodeficiency Virus (HIV) lihlala egazini lomuntu futhi lingabhubhisa amasosha omzimba emizimba yabantu, ngaleyo ndlela libenze balahlekelwe ukumelana nezinye izifo, okubangela izifo ezingelapheki kanye nezimila, futhi ekugcineni kuholele ekufeni. I-HIV ingadluliselwa ngokuxhumana ngokocansi, igazi, kanye nokudluliselwa komntwana kusuka kumama kuye kosana.
Isiteshi
| I-FAM | I-RNA ye-HIV |
| I-VIC(HEX) | Ukulawula kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | Amasampula e-Serum/Plasma |
| CV | ≤5.0% |
| Ct | ≤38 |
| I-LoD | 100 IU/mL |
| Ukucaciswa | Sebenzisa ikhithi ukuhlola amanye amasampula egciwane noma amabhaktheriya afana nalawa: i-human cytomegalovirus, igciwane le-EB, igciwane le-human immunodeficiency, igciwane le-hepatitis B, igciwane le-hepatitis A, i-syphilis, igciwane le-herpes simplex uhlobo 1, igciwane le-herpes simplex uhlobo 2, igciwane le-influenza A, i-staphylococcus aureus, i-candida albicans, njll., futhi imiphumela yonke ayimibi. |
| Izinsimbi Ezisebenzayo: | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-SLAN ®-96P Zesikhathi Sangempela Izinhlelo ze-PCR ze-QuantStudio™ ezi-5 zesikhathi sangempela Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ukukhipha kufanele kwenziwe ngokwencwadi yemiyalelo. Umthamo wesampula ungama-300μL, umthamo wokulungisa onconyiwe ungama-80μL.
