I-BCR-ABL Fusion Gene Mutation yabantu

I-BCR-ABL Fusion Gene Mutation Yomuntu Isithombe Esifakiwe

Incazelo emfushane:

Le khithi ifanele ukutholwa kwekhwalithi yama-isoforms we-p190, p210 kanye ne-p230 yofuzo oluhlanganisiwe lwe-BCR-ABL kumasampuli womnkantsha womuntu.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-GE010A-Human BCR-ABL Fusion Gene Mutation Detection Kit (Fluorescence PCR)

I-Epidemiology

I-Chronic myelogenousleukemia (CML) yisifo se-clonal esiyingozi samaseli e-hematopoietic stem.Ngaphezu kwe-95% yeziguli ze-CML ziphethe i-Philadelphia chromosome (Ph) kumaseli azo egazi.I-pathogenesis evelele ye-CML imi kanje: I-BCR-ABL fusion gene yenziwa ukudluliselwa phakathi kwe-abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) engalweni ende ye-chromosome 9 (9q34) kanye nesifunda seqoqo le-breakpoint ( BCR) isakhi sofuzo engalweni ende yechromosome 22 (22q11);iphrotheni ehlanganisiwe efakwe yileli sakhi sofuzo inomsebenzi we-tyrosine kinase (TK), futhi yenza kusebenze izindlela zayo zokusayina ezansi nomfula (njenge-RAS, PI3K, ne-JAK/STAT) ukuze kuthuthukiswe ukuhlukana kwamaseli futhi kuvimbele i-apoptosis yamaseli, okwenza amaseli anda ngendlela eyingozi, futhi ngaleyo ndlela abangele ukwenzeka kwe-CML.I-BCR-ABL ingenye yezinkomba ezibalulekile zokuxilonga ze-CML.Ushintsho oluguquguqukayo lwezinga layo lokuloba luyinkomba enokwethenjelwa yokwahlulela okubikezelwe kwe-leukemia futhi ingasetshenziswa ukubikezela ukuvela kabusha kwe-leukemia ngemva kokwelashwa.

Isiteshi

FAM	I-BCR-ABL yofuzo oluhlanganisiwe
VIC/HEX	Ukulawula Kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji	Uketshezi: ≤-18℃ ebumnyameni
Impilo yeshelufu	Uketshezi: izinyanga ezingu-9
Uhlobo lwesifanekiso	Amasampula omnkantsha
LoD	1000 amakhophi/mL
Ukucaciswa	Akukho ukusebenza kabusha okuphambene nezinye izinhlobo zofuzo ze-fusion TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, kanye ne-PML-RARa
Izinsimbi Ezisebenzayo	I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio® 5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System