Ukuguqulwa Kwezakhi Zofuzo Ze-BCR-ABL Ze-Human Fusion
Igama lomkhiqizo
Ikhithi Yokuthola Ukuguquka Kwezakhi Zofuzo Ye-HWTS-GE010A-Human BCR-ABL Fusion (i-Fluorescence PCR)
Ikhithi Yokuthola Ukuguquka Kwezakhi Zofuzo Yomuntu Eyomisiwe Ye-HWTS-GE016A (i-Fluorescence PCR)
I-Epidemiology
I-myelogenousleukemia engapheli (CML) yisifo esibi se-clonal samaseli e-hematopoietic stem. Ngaphezu kwama-95% eziguli ze-CML zithwala i-Philadelphia chromosome (Ph) emaseli azo egazi. I-pathogenesis eyinhloko ye-CML yile: I-BCR-ABL fusion gene yakhiwa ngokufuduka phakathi kwe-abl proto-oncogene (i-Abelson murine leukemia viral oncogene homolog 1) engalweni ende ye-chromosome 9 (9q34) kanye ne-breakpoint cluster region (BCR) gene engalweni ende ye-chromosome 22 (22q11); iphrotheni ye-fusion ebhalwe yileli gene inomsebenzi we-tyrosine kinase (TK), futhi isebenzisa izindlela zayo zokubonisa izimpawu ezingezansi (njenge-RAS, PI3K, kanye ne-JAK/STAT) ukukhuthaza ukuhlukaniswa kwamaseli nokuvimbela i-apoptosis yamaseli, okwenza amaseli ande ngendlela enobungozi, ngaleyo ndlela abangele ukwenzeka kwe-CML. I-BCR-ABL ingenye yezinkomba ezibalulekile zokuxilonga ze-CML. Ushintsho oluguquguqukayo lwezinga layo lokubhala luyisibonakaliso esithembekile sokwahlulela kokubikezela i-leukemia futhi lungasetshenziswa ukubikezela ukuphinda kwe-leukemia ngemva kokwelashwa.
Isiteshi
| I-FAM | I-gene ye-fusion ye-BCR-ABL |
| I-VIC/HEX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Uketshezi: izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | Amasampula omnkantsha |
| I-LoD | Amakhophi ayi-1000/mL |
| Ukucaciswa
| Akukho ukuhlangana kwezakhi zofuzo nezinye izakhi zofuzo ze-fusion i-TEL-AML1, i-E2A-PBX1, i-MLL-AF4, i-AML1-ETO, kanye ne-PML-RARa |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio® 5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
