Uhlobo lomuntu ongumuntu egfr 29 izinguquko
Igama lomkhiqizo
I-HWTS-TM00122Aman EGFR 29 I-Mutations Detection Kit (Fleorescence PCR)
I-Epidemiology
Umdlavuza wamaphaphu usephenduke imbangela ehamba phambili yokufa komdlavuza emhlabeni wonke, usongela impilo yabantu. Ama-accounces angewona ama-cell lungle amaphaphu ama-80% eziguli zomdlavuza wamaphaphu. Njengamanje yithagethi ebaluleke kakhulu yamangqamuzana ekwelapheni umdlavuza wamaphaphu okungewona omncane. I-phosphorylation ye-eGFR ingakhuthaza ukukhula kwamaseli esimila, umehluko, ukuhlasela, i-metastasis, anti-apoptosis, futhi kukhuthaze isimila seTesmor angiogenesis. I-EGFR Tyrosine Kinase inhibitors (TKI) ingavimba indlela ye-EGFR ukusayina ngokuvimbela i-EGFR apophosphorylation, ngaleyo ndlela kuvimbela ukwanda kwe-apoptosphorylation, ukukhulisa i-apoptosis ye-tumor, ukuthuthukisa isimila se-angoogenesis, njl., Ukuze kufinyelele ekwelashweni okuqondiswe emphakathini. Inani elikhulu lezifundo ezikhombisile ukuthi ukusebenza kahle kwezokwelapha kwe-EGFR-TKI kuhlobene kakhulu nesimo sokuguqulwa kwe-EGFR gene, futhi kungavimba ngokuqondile ukukhula kwamangqamuzana esimilani nge-EGFR gene aution. Uhlobo lwe-EGR lutholakala engalweni emfushane ye-chromosome 7 (7P12), ngobude obugcwele be-200KB futhi iqukethe ama-28 Exens. Isifunda esiguquliwe sitholakala ikakhulu kuma-18 kuya ku-21, amakhodi angama-746 kuye ku-753 ukusulwa kokuguqulwa kwama-exon 19 ama-akhawunti acishe abe ngu-45% ama-akhawunti angama-40 kuye kwangama-45%. Imihlahlandlela ye-NCCN yokuxilongwa kanye nokwelashwa komdlavuza wamaphaphu weseli ongewona omncane kusho ukuthi ukuhlolwa kwe-gene gene mege kuyadingeka ngaphambi kwe-EGFR-TKI Administration. Le kit yokuhlola isetshenziselwa ukuqondisa ukuphathwa kwe-Epidermal Grow Fitor Receptor Tyrosine Kinase Inhibitor (EGFR-TKI), futhi inikeze isisekelo semithi eqondene nomdlavuza ongewona amaphaphu. Le khithi isetshenziselwa kuphela ukutholwa kwezinguquko ezijwayelekile kuhlobo lwe-EGFR ezigulini ezinomdlavuza wamaphaphu okungewona amancane. Imiphumela yokuhlola ingeyereferensi yezempilo kuphela futhi akufanele isetshenziswe njengesisekelo sokwelashwa komuntu ngamunye kweziguli. Odokotela kufanele bacabangele isimo sesiguli, izinkomba zezidakamizwa, kanye nokwelashwa ukusabela nezinye izinkomba zokuhlola elebhu kanye nezinye izinto zisetshenziselwa ukwahlulela imiphumela yokuhlola.
Insungubezi
| -Yenza | I-IC reaction buffer, i-l858r yokuphendula i-buffer, i-19del reaction buffer, i-T790m Reacler Reacler, 360 Reaction buffer, i-L8611 Reacler Buffer, s768i Reacler Buffer |
Amapharamitha Ezobuchwepheshe
| Ukubeka | Uketshezi: ≤-18 ℃ ebumnyameni; I-Lyophile: ≤30 ℃ ebumnyameni |
| Ishalofu-impilo | Uketshezi: izinyanga eziyi-9; I-Lyophilised: Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Isicubu esisha se-tumo, isigaba se-pathological efriziwe, izicubu ze-paraffin-ezishunyekiwe ze-paraffed noma isigaba, i-plasma noma i-serum |
| CV | <5.0% |
| Indlu edayisa insimbi | Ukutholwa kwesixazululo se-nucleic acid ukusabela ngaphansi kwesizinda sohlobo lwe-3ng / μl lwasendle, kungathola ngokuqinile isilinganiso se-1% sokuguqulwa |
| Ukucacisa | Akukho okuphambana nokusebenza kabusha nge-DNA yohlobo lwabantu yasendle kanye nezinye izinhlobo ze-mutant |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMSKusetshenziswe ama-biosystem 7300 amasistimu wesikhathi sangempela se-PCR I-QuantStudio® 5 Systems real-time PCREMS I-Lightcycler® 480 Uhlelo lwe-PRCR yesikhathi sangempela I-Biorad CFX96 Uhlelo lwe-Real-Time PCR |
Ukugeleza komsebenzi
