Izinguquko ze-Gene 29 ze-EGFR zomuntu
Igama lomkhiqizo
Ikhithi Yokuthola Ukuguquka Kwezakhi Zofuzo Ze-HWTS-TM0012A-Human EGFR Gene 29 (i-Fluorescence PCR)
I-Epidemiology
Umdlavuza wamaphaphu ube yimbangela ehamba phambili yokufa komdlavuza emhlabeni jikelele, okusongela kakhulu impilo yabantu. Umdlavuza wamaphaphu ongewona amangqamuzana amancane ubanga cishe ama-80% eziguli zomdlavuza wamaphaphu. I-EGFR njengamanje iyisisulu esibaluleke kakhulu sama-molecule ekwelapheni umdlavuza wamaphaphu ongewona amangqamuzana amancane. I-phosphorylation ye-EGFR ingakhuthaza ukukhula kwamaseli esimila, ukuhlukanisa, ukuhlasela, ukusabalala, ukulwa ne-apoptosis, futhi ikhuthaze i-angiogenesis yesimila. Ama-inhibitors e-tyrosine kinase e-EGFR (i-TKI) angavimba indlela yokubonisa ye-EGFR ngokuvimbela i-EGFR autophosphorylation, ngaleyo ndlela avimbele ukwanda nokwehlukaniswa kwamaseli esimila, akhuthaze i-apoptosis yamaseli esimila, anciphise i-angiogenesis yesimila, njll., ukuze kufezwe ukwelashwa okuqondiswe ku-tumor. Inani elikhulu lezifundo likhombisile ukuthi ukusebenza kahle kokwelapha kwe-EGFR-TKI kuhlobene eduze nesimo sokuguqulwa kwezakhi zofuzo ze-EGFR, futhi kungavimbela ngqo ukukhula kwamaseli esimila ngokuguqulwa kwezakhi zofuzo ze-EGFR. I-gene ye-EGFR itholakala engalweni emfushane ye-chromosome 7 (7p12), enobude obuphelele obungu-200Kb futhi iqukethe ama-exons angu-28. Indawo eguquliwe itholakala kakhulu kuma-exons angu-18 kuya ku-21, ama-codons angu-746 kuya ku-753 deletion mutation ku-exon 19 angaba ngu-45% kanti i-L858R mutation ku-exon 21 iba ngu-40% kuya ku-45%. Iziqondiso ze-NCCN Zokuxilongwa Nokwelashwa Komdlavuza Wamaphaphu Ongewona Amancane Amaseli zisho ngokusobala ukuthi ukuhlolwa kokuguqulwa kwezakhi zofuzo ze-EGFR kuyadingeka ngaphambi kokuphathwa kwe-EGFR-TKI. Le khithi yokuhlola isetshenziselwa ukuqondisa ukuphathwa kwemithi yokukhula kwe-epidermal factor receptor tyrosine kinase inhibitor (EGFR-TKI), futhi ihlinzeka ngesisekelo semithi eyenzelwe wena iziguli ezinomdlavuza wamaphaphu ongewona amaseli amancane. Le khithi isetshenziselwa kuphela ukuthola izinguquko ezivamile ku-gene ye-EGFR ezigulini ezinomdlavuza wamaphaphu ongewona amaseli amancane. Imiphumela yokuhlolwa ingeyereferensi yomtholampilo kuphela futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa kweziguli okuqondene nomuntu ngamunye. Odokotela kufanele bacabangele isimo sesiguli, izinkomba zemithi, kanye nokwelashwa. Ukusabela kanye nezinye izinkomba zokuhlolwa kwelabhorethri kanye nezinye izici kusetshenziswa ukwahlulela ngokuphelele imiphumela yokuhlolwa.
Isiteshi
| I-FAM | I-IC Reaction Buffer, i-L858R Reaction Buffer, i-19del Reaction Buffer, i-T790M Reaction Buffer, i-G719X Reaction Buffer, i-3Ins20 Reaction Buffer, i-L861Q Reaction Buffer, i-S768I Reaction Buffer |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Uketshezi: izinyanga ezingu-9; I-Lyophilized: izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | izicubu zesimila esisha, ingxenye yesifo esiqandisiwe, izicubu noma ingxenye yesifo esifakwe iparafini, iplasma noma i-serum |
| CV | ＜5.0% |
| I-LoD | Ukutholwa kwesisombululo sokusabela kwe-nucleic acid ngaphansi kwesizinda se-3ng/μL wild-type, kungabona izinga lokuguqulwa kwe-1% ngokuzinzile |
| Ukucaciswa | Akukho ukuhlangana kwe-DNA yomuntu yofuzo lohlobo lwasendle kanye nezinye izinhlobo eziguquliwe |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-TimeIzinhlelo ze-PCR ze-Applied Biosystems 7300 Real-Time Izinhlelo ze-PCR ze-QuantStudio® 5 Zesikhathi Sangempela Uhlelo lwe-PCR lwe-LightCycler® 480 lwesikhathi sangempela Uhlelo lwe-BioRad CFX96 Real-Time PCR |
Ukugeleza Komsebenzi
