Ukuguqulwa kohlobo lwe-gebheli lwabantu lwe-EML4-ALK Fusion
Igama lomkhiqizo
I-HWTS-TM006-Human Fusion Gene Mutation Detection Dection Dection
Isitifiketi
Tfda
I-Epidemiology
Le khithi isetshenziselwa ukuthola kahle izinhlobo eziyi-12 zokuguqulwa kwe-EML4-ALK Fusion Gene kumasampula eziguli ezinomdlavuza we-Human ongewona amaphaphu e-Vitro. Imiphumela yokuhlola ingeyereferensi yezempilo kuphela futhi akufanele isetshenziswe njengesisekelo sokwelashwa komuntu ngamunye kweziguli. Odokotela kufanele benze izinqumo eziphelele emphumeleni wokuhlola ngokuya ngezinto ezinjengesimo sesiguli, izinkomba zezidakamizwa, impendulo yokwelashwa, nezinye izinkomba zokuhlola zelebhu. Umdlavuza wamaphaphu yikhona isiminya elijwayelekile kunazo zonke emhlabeni jikelele, futhi ama-80% ~ 85% amacala angenamdlandla umdlavuza wama-cell ansundu (NSCLC). I-Gene Fusion ye-Echinoderm Microtubule-ahlobene ne-4 (EML4) ne-AnaPlastic Lymphoma Kinase (ALK4) i-Anaplastic Lymphoma Kinase (EML4 ne-ALK atholakala ngokulandelana ku-P21 nama-P23 ku-Chromosome 2 futhi ahlukaniswe cishe nge-12.7 izisekelo eziyisisekelo ngazimbili. Okungenani okungu-20 Ukuhlukahluka kokufuya okutholakele, phakathi kwalo, phakathi kwalo i-Retant Returants Mutants etafuleni 1 kuvamile, lapho i-Mutant 1 (E13; A20) ivame kakhulu, ilandelwa yi-Mutants 3a ne-3B (E6; A20), i-accounting mayelana Ama-33% no-29% weziguli ezine-EML4-ALK Fusion Gene NSCLC, ngokulandelana. I-Alk inhibitors emelwe yiCrizotinib yimithi emincane ebhekiswe emangalweni ebhekiswe ekwakhelwe izinguquko ze-Alk Gene Fusion. Ngokuvimbela umsebenzi we-ALK Tyrosine Kinase Region, uvimbele imigwaqo yayo ebonakalayo engajwayelekile, kuvimbela ukukhula kwamangqamuzana esimila, ukuze kufinyelelwe ukwelashwa okuhlosiwe kwama-tumors. Ucwaningo lweMitholampilo lukhombisile ukuthi i-Crizotinib inezinga eliphumelelayo langaphezu kwama-61% ezigulini ezine-EML4-ALK Fusion Delikation, ngenkathi icishe ibe nomthelela ezigulini zohlobo lwasendle. Ngakho-ke, ukutholwa kokuguqulwa kwe-EML4-ALK Fusion Ukuguqulwa kwe-EML4-ALK yiso isisekelo nesisekelo sokuqondisa ukusetshenziswa kwezidakamizwa ze-crizotinib.
Insungubezi
| -Yenza | Ukuphendula Buffer 1, 2 |
| I-VIC (Hex) | I-Reaction Buffer 2 |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | I-Paraffin-Eshunyekwe Eshunyekwe Ngama-Pathological Aispological noma Amasampula Wesigaba |
| CV | <5.0% |
| Ct | ≤38 |
| Indlu edayisa insimbi | Le khithi ingabona izinguquko eziguqukayo njengamakhophi angama-20. |
| Izinsimbi ezisebenzayo: | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR SLAN ®-96P real-time PCRS Systems I-QuantStudio ™ 5 Izinhlelo ze-PCR zangempela zesikhathi sangempela I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-realent enconyiwe: I-RNEESY FFPE KIT (73504 Kit (73504) ngama-Qiagen izingxenye zezicubu ze-RNA Extraction Kit (DP439) nguTiangen Biotech (Beijing) Co, Ltd.
