I-EML4-ALK Fusion Gene Mutation yomuntu
Igama lomkhiqizo
I-HWTS-TM006-Human EML4-ALK Fusion Gene Mutation Detection Kit(Fluorescence PCR)
Isitifiketi
I-TFDA
I-Epidemiology
Le khithi isetshenziselwa ukuthola ngokufanelekile izinhlobo eziyi-12 eziguqukayo zofuzo oluhlanganisiwe lwe-EML4-ALK kumasampuli eziguli zomdlavuza wamaphaphu ongewona omncane we-vitro.Imiphumela yokuhlolwa ingeyenkomba yomtholampilo kuphela futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa komuntu ngamunye kweziguli.Odokotela kufanele benze izinqumo ezibanzi emiphumeleni yokuhlolwa ngokusekelwe ezicini ezifana nesimo sesiguli, izinkomba zomuthi, ukusabela kokwelashwa, nezinye izinkomba zokuhlola elabhorethri.Umdlavuza wamaphaphu uyisimila esiyingozi esivame kakhulu emhlabeni wonke, kanti u-80% ~85% wamacala umdlavuza wamaphaphu ongewona omncane (NSCLC).I-Gene fusion ye-echinoderm microtubule-associated protein-like 4 (EML4) kanye ne-anaplastic lymphoma kinase (ALK) iyinoveli eqondiwe ku-NSCLC, EML4 kanye ne-ALK ngokulandelanayo atholakala kubantu amabhande e-P21 kanye ne-P23 ku-chromosome 2 futhi ahlukaniswa cishe ngo-12.7 million base pairs.Okungenani izinhlobonhlobo ze-fusion ezingu-20 zitholiwe, phakathi kwazo okuguquguqukayo okungu-12 kuThebula 1 kuvame, lapho i-mutant 1 (E13; A20) ivame kakhulu, ilandelwa i-mutant 3a kanye ne-3b (E6; A20), ibalwa cishe U-33% no-29% weziguli ezine-EML4-ALK fusion gene NSCLC, ngokulandelana.Ama-ALK inhibitors amelelwa i-Crizotinib imithi eqondiswe kuma-molecule amancane enzelwe ukuguqulwa kofuzo lwe-ALK.Ngokuvimbela umsebenzi wesifunda se-ALK tyrosine kinase, sivimbe izindlela zayo zokubonisa ezingavamile ezansi nomfula, ngaleyo ndlela kuvinjwe ukukhula kwamangqamuzana esimila, ukuze kuzuzwe ukwelashwa okuhlosiwe kwamathumba.Ucwaningo lwezokwelapha lubonise ukuthi i-Crizotinib inenani elisebenzayo elingaphezu kuka-61% ezigulini ezinokuguqulwa kwe-EML4-ALK fusion, kuyilapho ingenawo umthelela ezigulini zohlobo lwasendle.Ngakho-ke, ukutholwa kokuguqulwa kokuhlanganiswa kwe-EML4-ALK kuyisisekelo nesisekelo sokuqondisa ukusetshenziswa kwezidakamizwa ze-Crizotinib.
Isiteshi
| FAM | Isilondolozi sokusabela 1, 2 |
| I-VIC(HEX) | Isilondolozi sokusabela 2 |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo lwesifanekiso | izicubu ze-pathological ezishumekwe upharafini noma amasampula esigaba |
| CV | ＜5.0% |
| Ct | ≤38 |
| LoD | Le khithi ingathola ukuguqulwa kokuhlanganiswa okuphansi okungamakhophi angama-20. |
| Izinsimbi Ezisebenzayo: | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-SLAN ®-96P Real-Time PCR Systems I-QuantStudio™ 5 Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: RNeasy FFPE Kit (73504) by QIAGEN, Izigaba Zezicubu Ezishumekwe Upharafini Isamba Sekhithi Yokukhipha I-RNA(DP439) yi-Tiangen Biotech(Beijing) Co.,Ltd.
