Ukuguqulwa Kwezakhi Zofuzo Ze-PML-RARA Zomuntu
Igama lomkhiqizo
I-HWTS-TM017AIkhithi Yokuthola Ukuguquka Kwezakhi Zofuzo Ze-PML-RARA Yomuntu (i-Fluorescence PCR)
I-Epidemiology
I-acute promyelocytic leukemia (APL) uhlobo olukhethekile lwe-acute myeloid leukemia (AML). Cishe ama-95% eziguli ze-APL ahambisana noshintsho olukhethekile lwe-cytogenetic, okungukuthi i-t(15;17)(q22;q21), okwenza i-PML gene ku-chromosome 15 kanye ne-retinoic acid receptor α gene (RARA) ku-chromosome 17 kuhlanganiswe ukwakha i-PML-RARA fusion gene. Ngenxa yezindawo ezahlukene zokwaphuka kwe-PML gene, i-PML-RARA fusion gene ingahlukaniswa ngohlobo olude (uhlobo lwe-L), uhlobo olufushane (uhlobo lwe-S) kanye nohlobo oluhlukile (uhlobo lwe-V), olubalelwa cishe ku-55%, 40% kanye no-5% ngokulandelana.
Isiteshi
| I-FAM | I-gene ye-fusion ye-PML-RARA |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | umkantsha |
| CV | <5.0％ |
| I-LoD | Amakhophi ayi-1000/mL. |
| Ukucaciswa | Akukho ukuhlangana kwezakhi zofuzo ze-fusion nezinye izakhi zofuzo ze-fusion ze-BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, kanye ne-TEL-AML1. |
| Izinsimbi Ezisebenzayo | Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-reagent yokukhipha enconywayo: I-RNAprep Pure Blood Total RNA Extraction Kit (DP433). Ukukhipha kufanele kwenziwe ngokwe-IFU.
