Human PML-RARA Fusion Gene Mutation
Igama lomkhiqizo
I-HWTS-TM017AIkhithi Yokuthola I-PML-RARA Fusion Gene Mutation Detection (Fluorescence PCR)
I-Epidemiology
I-acute promyelocytic leukemia (APL) wuhlobo olukhethekile lwe-acute myeloid leukemia (AML). Cishe i-95% yeziguli ze-APL zihambisana noshintsho olukhethekile lwe-cytogenetic, okungukuthi t(15;17)(q22;q21), okwenza isakhi sofuzo se-PML ku-chromosome 15 kanye ne-retinoic acid receptor α gene (RARA) ku-chromosome 17 ehlanganiswe ukwenza isakhi sofuzo se-PML-RARA. Ngenxa yokuhlukana okuhlukene kofuzo lwe-PML, isakhi sofuzo esihlanganisiwe se-PML-RARA singahlukaniswa sibe uhlobo olude (uhlobo L), uhlobo olufushane (uhlobo S) nohlobo oluhlukile (uhlobo lwe-V), olubalelwa cishe ku-55%, 40% no-5% ngokulandelanayo.
Isiteshi
| FAM | I-PML-RARA yokuhlanganisa isakhi sofuzo |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ ebumnyameni |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | umkantsha |
| CV | <5.0％ |
| LoD | 1000 Amakhophi/mL. |
| Ukucaciswa | Akukho ukusebenza kabusha okuphambene nezinye izinhlobo zofuzo ze-fusion BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, kanye ne-TEL-AML1 yofuzo |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-RNAprep I-Pure Blood Total RNA Extraction Kit (DP433). Ukukhishwa kufanele kwenziwe ngokwe-IFU.
