Ukuguqulwa kohlobo lwe-PML-Rara
Igama lomkhiqizo
Hwts-tm017aI-Human PML-Rara Fusion Gene Mutation Detection Dection Dection (Fluorescence PCR)
I-Epidemiology
I-Acute PromoyeloCytic Leukemia (APL) iyindlela ekhethekile ye-acute auteyoid leukemia (AML). Cishe ama-95% eziguli ze-APL ahambisana noshintsho olukhethekile lwe-cytogenetic, okungukuthi u-T (15; 17; 17) (Q22; yakha uhlobo lwe-PML-Rara Fusion. Ngenxa yemibhalo ehlukene ye-gene ye-PML, i-PML-Rara Fusion Gene ihlukaniswe ngohlobo olude (uhlobo lwe-L), uhlobo olufushane (uhlobo lwe-Type) kanye nohlobo lwe-accounting olungafani no-40%, 40% no-5 % ngokulandelana.
Insungubezi
| -Yenza | I-PML-Rara Fusion Gene |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ ebumnyameni |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | umkantsha |
| CV | <5.0% |
| Indlu edayisa insimbi | Amakhophi ayi-1000 / ml. |
| Ukucacisa | Akukho okuphambana nokuphindaphindeka okunamanye ama-Fusion Gerses Bcr-abl, e2a-PBX1, MLL-AF4, AML1-ETO |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 uhlelo lwe-real-time PCR Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reaglent enconyiwe ye-realent: I-RNApRep emsulwa yegazi eliphelele le-RNA elithi Ikhithi (DP433). Ukukhishwa kufanele kwenziwe ngokuya nge-IFU.
