Izinhlobo ezingama-28 ze-Human Papilloma Virus (16/18 Typing) esengozini enkulu ye-Nucleic Acid
Igama lomkhiqizo
I-HWTS-CC006A-28 Izinhlobo Zegciwane Le-Human Papilloma Virus (16/18 Typing) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungesinye sezimila eziyingozi ezivame kakhulu emgudwini wokuzala wabesifazane.Ucwaningo luye lwabonisa ukuthi izifo ezihlala njalo ze-HPV kanye nezifo eziningi zingenye yezimbangela eziyinhloko zomdlavuza womlomo wesibeletho.Njengamanje, izindlela zokwelapha ezisebenzayo ezibonwayo azikatholakali umdlavuza womlomo wesibeletho obangelwa i-HPV, ngakho ukutholakala kusenesikhathi kanye nokuvinjelwa kokutheleleka komlomo wesibeletho okubangelwa yi-HPV kuwukhiye wokuvimbela umdlavuza womlomo wesibeletho.Kubaluleke kakhulu ukusungula isivivinyo sokuxilonga i-etiology elula, eqondile futhi esheshayo ukuze kuhlonzwe umtholampilo kanye nokwelashwa komdlavuza womlomo wesibeletho.
Isiteshi
| Ukusabela Mix | Isiteshi | Uhlobo |
| I-PCR-Mix1 | FAM | 18 |
| I-VIC(HEX) | 16 | |
| I-ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Ukulawula Kwangaphakathi | |
| I-PCR-Mix2 | FAM | 6, 11, 54, 83 |
| I-VIC(HEX) | 26, 44, 61, 81 | |
| I-ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | I-Cervical Swab, I-Vaginal Swab, Umchamo |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300Amakhophi/mL |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test I-Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-200μL wosawoti ojwayelekile ukuze umise okwesikhashana i-pellet esinyathelweni sesi-2.1, bese ukukhishwa kufanele kwenziwe ngokuvumelana emiyalweni yokusetshenziswa kwalesi sici sokukhipha.Ivolumu ye-elution enconyiwe ngu-80μL.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-QIAamp DNA Mini Kit (51304) noma i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Engeza u-200μL we-saline evamile ukuze umise kabusha i-pellet esinyathelweni sesi-2.1, bese ukukhishwa kufanele kwenziwe ngokuvumelana nemiyalelo yokusetshenziswa kwalesi sici sokukhipha.Ivolumu yesampula ekhishiwe yamasampula wonke angu-200μL, futhi ivolumu ye-elution enconyiwe ingu-100μL.
