Izinhlobo ezingu-28 ze-Human Papilloma Virus eyingozi kakhulu (uhlobo lwe-16/18) i-Nucleic Acid
Igama lomkhiqizo
Izinhlobo ze-HWTS-CC006A-28 ze-Human Papilloma Virus eyingozi kakhulu (16/18 Typing) I-Nucleic Acid Detection Kit (i-Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu ohlelweni lokuzala lwabesifazane. Izifundo zikhombisile ukuthi ukutheleleka okuqhubekayo kwe-HPV kanye nokutheleleka okuningi kungenye yezimbangela eziyinhloko zomdlavuza womlomo wesibeletho. Njengamanje, ukwelashwa okusebenzayo okuqashelwayo kusantuleka komdlavuza womlomo wesibeletho obangelwa yi-HPV, ngakho-ke ukutholakala kusenesikhathi nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa yi-HPV kuyisihluthulelo sokuvimbela umdlavuza womlomo wesibeletho. Kubaluleke kakhulu ukusungula isivivinyo sokuxilonga esilula, esiqondile nesisheshayo se-etiology sokuxilongwa kanye nokwelashwa komdlavuza womlomo wesibeletho.
Isiteshi
| Ingxube Yokusabela | Isiteshi | Uhlobo |
| I-PCR-Mix1 | I-FAM | 18 |
| I-VIC(HEX) | 16 | |
| I-ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Ukulawula Kwangaphakathi | |
| I-PCR-Mix2 | I-FAM | 6, 11, 54, 83 |
| I-VIC(HEX) | 26, 44, 61, 81 | |
| I-ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Swab Yesibeletho, I-Swab Yesitho Sangasese Sowesifazane, Umchamo |
| Ct | ≤28 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-300/mL |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Bheka i-Kit IFU ukuthola imininingwane
Isixazululo 1 (Ukukhishwa kwe-nucleic acid okuzenzakalelayo)
Isixazululo 2: (I-Macro & Micro-Test Sample Release Release Reagent)
Ama-reagent adinga kodwa awanikezwa
I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ikholomu ye-Macro & Micro-Test Viral DNA/RNA (HWTS-3020-50).
Izinto ezisetshenziswayo ziyadingeka kodwa azinikezwa
Amashubhu e-centrifuge angenayo i-DNase/RNase angu-1.5mL, Amathiphu angenayo i-DNase/RNase, amashubhu e-PCR angu-0.2mL, i-desktop centrifuge, i-desktop oscillation mixer.
