Ukuguqulwa Kwezakhi Zofuzo Ze-TEL-AML1 Zomuntu
Igama lomkhiqizo
Ikhithi Yokuthola Ukuguquka Kwezakhi Zofuzo Ye-HWTS-TM016 Yomuntu i-TEL-AML1 Fusion (i-Fluorescence PCR)
I-Epidemiology
I-acute lymphoblastic leukemia (ALL) iyona evame kakhulu ebuntwaneni. Eminyakeni yamuva nje, i-acute leukemia (AL) ishintshe kusukela ohlotsheni lwe-MIC (i-morphology, i-immunology, i-cytogenetics) kuya ohlotsheni lwe-MICM (ukwengezwa kokuhlolwa kwe-molecular biology). Ngo-1994, kwatholakala ukuthi ukuhlangana kwe-TEL ebuntwaneni kwakubangelwa ukudluliselwa kwe-chromosomal okungahleliwe t(12;21)(p13;q22) ku-B-lineage acute lymphoblastic leukemia (ALL). Kusukela kwatholakala i-AML1 fusion gene, i-TEL-AML1 fusion gene iyindlela engcono kakhulu yokwahlulela ukubikezela kwezingane ezine-acute lymphoblastic leukemia.
Isiteshi
| I-FAM | I-gene ye-fusion ye-TEL-AML1 |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | isampula yomnkantsha |
| Ct | ≤40 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-1000/mL |
| Ukucaciswa | Akukho ukuhlangana phakathi kwama-kits kanye nezinye izakhi zofuzo zokuhlanganisa ezifana ne-BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, PML-RARa fusion genes. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Ikhithi Yokukhipha I-RNAprep Pure Blood Total RNA (DP433).
