Ukuguqulwa kwe-Human Tel-Aml1 Fusion Gene
Igama lomkhiqizo
I-HWTS-TM016 Fusion gener gene gene Mutation Detection Ikhithi (Fluorescence PCR)
I-Epidemiology
I-acute lymphoblastic leukemia (konke) iyona ebuntwaneni evame kakhulu ebuntwaneni. Eminyakeni yamuva nje, i-leukemia ebukhali (i-al) isishintshile isuka ku-mic (morphology, i-immunology, ama-cytogenetics) kuhlobo lwe-micm (ukungezwa kokuhlolwa kwe-molecular biology). Ngo-1994, kwatholakala ukuthi i-FEL fusion ebuntwaneni ibangelwa ukuhanjiswa kwe-chromomoral kwe-nonrandom chromomosomal t (12; 21) (p13) (P13; Kusukela ukutholwa kwe-AML1 Fusion Gene, i-Tel-Aml1 Fusion Gene iyindlela engcono kakhulu yokwahlulela i-Prognosis yezingane i-Lymphoblastic Leukemia.
Insungubezi
| -Yenza | I-Tel-Aml1 Fusion Gene |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | Isampula Sonamba |
| Ct | ≤40 |
| CV | <5.0% |
| Indlu edayisa insimbi | 1000copies / ml |
| Ukucacisa | Akukho okuphambana nokuphindaphindeka phakathi kwamakhithi nezinye izinhlobo zezithayiya ezinjenge-BCR-ABL, i-E2A-PBX1, MLL-AF4, i-AML1-ETO, i-PML-Rara, i-PML-Rara, i-PML-Rara genes Genes. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-Rnaprep emsulwa igazi eliphelele le-RNA elikhipha ikhithi (DP433).
