I-Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF)，Isoniazid Resistance(INH)

Incazelo emfushane:

Lo mkhiqizo ulungele ukutholwa kwekhwalithi ye-Mycobacterium tuberculosis DNA kumasampuli esikhwehlela somuntu ku-vitro, kanye nokuguqulwa kwe-homozygous endaweni engu-507-533 amino acid codon (81bp, indawo enquma ukumelana ne-rifampicin) yofuzo lwe-rpoB olubangela isifo sofuba i-Mycobacterium. ukumelana ne-rifampicin.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)

I-Epidemiology

I-Mycobacterium tuberculosis, kafushane nje njenge-Tubercle bacillus (TB), igciwane le-pathogenic elidala isifo sofuba.Njengamanje, imithi elwa nesifo sofuba esetshenziswa kakhulu yomugqa wokuqala ihlanganisa i-isoniazid, i-rifampicin ne-ethambutol, njll. Imithi yomugqa wesibili yokulwa nesifo sofuba ihlanganisa i-fluoroquinolones, i-amikacin ne-kanamycin, njll. Imithi emisha esanda kwakhiwa i-linezolid, i-bedaquiline ne-delamani, njll. Nokho, ngenxa yokusetshenziswa okungalungile kwemithi elwa nesifo sofuba kanye nezici zesakhiwo sodonga lwamangqamuzana e-mycobacterium tuberculosis, isifo sofuba se-mycobacterium siqala ukumelana nemithi elwa nesifo sofuba, okuletha izinselele ezinkulu ekuvimbeleni nasekwelapheni isifo sofuba.

Isiteshi

Igama Eliqondisiwe			Intatheli	Quencher
Isivimbeli sokusabelaA	Isivimbeli sokusabelaB	Isivimbeli sokusabelaC	Intatheli	Quencher
I-rpoB 507-514	I-rpoB 513-520	IS6110	FAM	Lutho
I-rpoB 520-527	rpoB 527-533	/	CY5	Lutho
/	/	Ukulawula kwangaphakathi	I-HEX(VIC)	Lutho

Isivimbeli sokusabelaD	Intatheli	Quencher
Isifunda somgqugquzeli we-InhA -15C>T, -8T>A, -8T>C	FAM	Lutho
I-KatG 315 codon 315G>A,315G>C	CY5	Lutho
Isifunda somgqugquzeli we-AhpC -12C>T, -6G>A	I-ROX	Lutho

Imingcele Yezobuchwepheshe

Isitoreji	≤-18℃
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	isikhwehlela
CV	≤5.0%
LoD	I-LOD ye-mycobacterium tuberculosis inkomba kazwelonke ngamabhaktheriya angama-50/mL.I-LoD yohlobo lwasendle olumelana ne-rifampicin inkomba kazwelonke ithi 2×10³amagciwane/mL, futhi i-LoD yohlobo oluguquguqukayo ingu-2×10³amagciwane/mL.I-LoD yohlobo lwasendle lwamagciwane amelana ne-isoniazid ngu-2x10³amagciwane/mL, kanye ne-LoD yebhaktheriya eguquguqukayo ingu-2x10³amagciwane/mL.
Ukucaciswa	Imiphumela yokuhlolwa kwesiphambano yabonisa ukuthi kwakungekho ukusabela okuphambene ekutholweni kwe-genome yomuntu, amanye amagciwane angewona ama-mycobacteria okungewona isifo sofuba kanye ne-pneumonia ngale kit;Kwakungekho ukusabela okuphambene okutholwe ezindaweni zokuguqulwa kwezinye izakhi zofuzo ezimelana nezidakamizwa kuhlobo lwasendle lwe-Mycobacterium tuberculosis.
Izinsimbi Ezisebenzayo	I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), I-BioRad CFX96 Real-Time PCR System, Ubuchwepheshe be-Hangzhou Bioer I-QuantGene 9600 Real-Time PCR System, I-QuantStudio^®5 Uhlelo lwe-PCR Yesikhathi Sangempela.