Ikhithi Yokuthola I-Nucleic Acid ye-Bundybugyo Virus Ebola (i-Fluorescence PCR)
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-Bundybugyo Virus Ebola (i-Fluorescence PCR)
I-Epidemiology
I-Filoviridae. Yigciwane lesithathu le-Ebola elingabangela ubhubhane olukhulu kubantu,ngemuva kwe-Zaire ebolavirus (EBOV) kanye ne-Sudan ebolavirus (SUDV). I-BDBV iyigciwane elilodwaigciwane le-RNA elinomugqa ongemuhle eline-genome engaba ngu-19 kb, elifaka ikhodi engu-7isakhiwoamaprotheni kanye namaprotheni amabili angewona ahlelekile. Isikhathi sokufukamela ukutheleleka kwe-BDBV singu-2–21izinsuku (isilinganiso sezinsuku ezingu-6.3). Izimpawu zayo zifana kakhulu nezezinye izifo ze-Ebola.futhi ayikwazi ukuhlukaniswa ngezibonakaliso zemitholampilo kuphela. Ngo-Agasti 2007,Ukuqubuka komkhuhlane wegazi obangelwa yigciwane ongachazeki kwenzeke eSifundeni saseBundibugyo saseentshonalanga ye-Uganda. Yatholakala njengegciwane elisha le-Ebola futhi yaqanjwa ngokuthi iBundibugyoi-ebolavirus (BDBV). Kube nezigameko ezingu-93 okusolwa ukuthi zingenwe yilesi sifo, amacala angu-56 aqinisekisiwe, kanye nokufa kwabantu abangu-37.(izinga lokufa kwabantu lingama-25%). Ngo-2012, kwaqubuka okunye ukuqubuka kwesifo eSifundazweni sase-Orientale e-IDemocratic Republic of the Congo, enamacala angu-38 aqinisekisiwe kanye nokufa okungu-13 (ukufa kwaleli calaizinga lama-34%. NgoMeyi 2026, i-WHO yamemezela ukuqubuka kwe-BDBV kwamanje e-IDemocratic Republic of the Congo kanye ne-Uganda yisimo esiphuthumayo sezempilo yomphakathiI-International Concern (PHEIC). Kusukela ngomhlaka-20 Meyi, kwakunezigameko ezisolwayo ezingu-246 kanye nezingu-80abashonile.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | 12izinyanga |
| Uhlobo Lwesifanekiso | igazi eliphelele, i-serum/i-plasma,oi-ral swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | 3Amakhophi angu-00/mL |
| Izinsimbi Ezisebenzayo | Uhlobo I:Uhlelo lwe-ABI 7500/7500 lwe-PCR olusheshayo lwesikhathi sangempela, Uhlelo lwe-PCR lwe-QuantStudio®5 lwesikhathi sangempela, Uhlelo lwe-PCR lwe-QuantStudio®7 lwesikhathi sangempela, Uhlelo Lokuhlaziya i-PCR Yezokwelapha lwe-Hongshi SLAN-96P/S Oluzenzakalelayo Ngokugcwele, Uhlelo lwe-PCR Lwesikhathi Sangempela lwe-LightCycler®480, Uhlelo lwe-PCR lwe-Hangzhou Bioer FQD-96A lwesikhathi sangempela, Uhlelo lwe-Yarui MA-6000 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Uhlobo II: Uhlelo Lokuthola Ama-Molecular Okuzenzakalelayo lwe-Eudemon AIO800 (HWTS-AIO800)
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Ukugeleza komsebenzi:
Uhlobo I:
Uhlobo II:
Ama-Reagents azilungiselele wona: I-saline yemvelo, ama-reagent okukhipha i-nucleic acid
Izinto Ezisetshenziswayo Ezizilungiselelayo: Amathiphu epayipi angenayo i-DNase/RNase, amagilavu angasetshenziswa, 1.5 mL
Amashubhu e-centrifuge angenayo i-DNase/RNase, i-centrifuge







