Igciwane le-Influenza A/ Igciwane le-Influenza B
Igama lomkhiqizo
I-HWTS-RT176-I-Influenza A Virus/Influenza B Virus eyomisiwe ngokuqandisa i-Nucleic Acid DetectionKit (i-Fluorescence PCR)
Ukuvunyelwa Komkhiqizo
I-IVDR
I-Epidemiology
Ngokusho komehluko we-antigenic phakathi kwezakhi zofuzo ze-NP ne-M, amagciwane omkhuhlaneingahlukaniswa ngezinhlobo ezine: igciwane le-influenza A (IFV A), igciwane le-influenza B (IFV B),i-influenza C virus (IFV C) kanye ne-influenza D virus (IFV D) [1]. Ngegciwane lomkhuhlane A,inezinhlobo eziningi zezidalwa kanye nama-serotype ayinkimbinkimbi futhi ingasakazeka kuzo zonke izilwane ngokusebenzisaukuhlanganiswa kwezakhi zofuzo kanye nokuguqulwa kwezakhi zofuzo okuguquguqukayo. Abantu abanawo amasosha omzimba ahlala njaloIgciwane le-influenza A, ngakho-ke abantu babo bonke ubudala ngokuvamile basengozini. Igciwane le-Influenza Ayizifo ezibangela ubhubhane lwe-influenza olukhulu kakhulu [2]. Ngegciwane le-influenza B,isakazeka kakhulu ezindaweni ezincane futhi okwamanje ayinazo izinhlobo ezincane.izifo zibangelwa kakhulu amagciwane omkhuhlane e-B/Yamagata noma i-B/Victoriaizizukulwane. Phakathi kwamacala omkhuhlane aqinisekisiwe nyanga zonke emazweni ayi-15 e-Isifunda sase-Asia-Pacific, izinga lokutholakala kwegciwane le-influenza B lisukela ku-0 kuya ku-92%[3]. Ngokungafani negciwane le-influenza A, amaqembu athile abantu, njengezingane kanyeasebekhulile, basengozini yokuthola igciwane le-influenza B, okungadala izinkinga kalula,okubangela imithwalo eminingi emphakathini kunegciwane le-influenza A[4].Umphumela ongemuhle awukuvimbeli ukutheleleka kwe-IFV A/B futhi akufanele kusetshenziswe njenge-isisekelo esisodwa sokuxilongwa, ukwelashwa, noma ezinye izinqumo zokuphatha.Umphumela awukuvimbeli ukutheleleka ngamagciwane noma okunye ukutheleleka ngamagciwane ngaphandle kwe-IFVIgciwane le-A/B.
Amapharamitha Obuchwepheshe
| Isitoreji | 2-28℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-swab ye-oropharyngeal yomuntu, i-nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-200/mL noma u-0.05TCID50/mL |
| Ukucaciswa | Ukusabela okuxubile: Akukho ukusabela okuxubile phakathi kwekhithi ne-Bocavirus, igciwane lokuphefumula elihambisana nokuphefumula uhlobo B, igciwane le-Herpes simplex uhlobo 1, i-Enterovirus, i-Adenovirus (Uhlobo 1), i-Adenovirus (Uhlobo 3), i-Adenovirus (Uhlobo 7), i-SARS-CoV, i-Adenovirus (Uhlobo 1), i-Adenovirus (Uhlobo 3), i-Adenovirus (Uhlobo 7), i-SARS-CoV, i-Norovirus, i-Streptococcus pneumoniae, i-Legionella, i-Bordetella pertussis, i-Neisseria gonorrhoeae, i-Aspergillus fumigatus, i-Moraxella catarrhalis, i-Rhinovirus, igciwane le-Parainfluenza (Uhlobo 1), igciwane le-Parainfluenza (Uhlobo 3), igciwane le-Parainfluenza (Uhlobo 2), igciwane le-Varicella-zoster, igciwane le-Measles, i-Human coronaviruses, i-MERS-CoV, i-Chlamydia pneumoniae, i-Klebsiella pneumoniae, i-Pneumocystis jirovecii, i-Staphylococcus aureus, i-Candida ama-albicans, ama-Cryptococcus neoformans, i-Lactobacillus, i-Cytomegalovirus, igciwane le-Epstein-Barr, igciwane le-Mumps, igciwane le-Human metapneumovirus (A1), igciwane le-Human metapneumovirus (B2), i-coronavirus entsha ka-2019-nCoV, i-Rotavirus, i-Mycoplasma pneumoniae, i-Streptococcus pyogenes, i-Haemophilus influenzae, i-Mycobacterium tuberculosis, i-Candida glabrata, i-Streptococcus salivarius, i-Corynebacterium, igciwane le-Influenza A, igciwane le-Influenza B, i-Human genomic DNA |
| Izinsimbi Ezisebenzayo | Uhlobo I: Izinhlelo ze-Biosystems ezisetshenzisiwe 7500 Real-Time PCR, SLAN-96PIzinhlelo ze-PCR Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.). Ukuhlolwa kohlobo lwesibili: i-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro kanye I-Micro-Test Med-Tech Co., Ltd. |
Ukugeleza komsebenzi
Uhlobo I: Kuhlelo lwe-PCR olujwayelekile
Uhlobo II: Ku-EudemonTMI-AIO800
Kudingeka ama-reagent/izinsimbi kodwa azinikezwanga
1) Ikhithi Yokukhipha i-RNA: Ikhithi ye-DNA/RNA ye-Macro & Macro & Micro-Test Viral(HWTS-3017)(engasetshenziswa ne-Macro & Micro-Test Automatic NucleicI-Acid Extractor (HWTS-EQ011)) yiJiangsu Macro & Micro-Test Med-Tech Co.,Ltd., Ikhithi ye-Macro & Micro-Test Viral DNA/RNA (HWTS-3017-8) (esetshenziswa ne-I-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro kanye ne-Micro-Test Med-TechI-Co., Ltd.
2) Isixazululo Sokulondolozwa Kwesampula: Ikhithi Yokusampula Amagciwane (JXZZ20182400236) nguI-Yocon Biology (Beijing) Co., Ltd., UTM® Universal Transport Medium™(GXB20191277) yiCopan Italia SpA, Ukuqoqwa Kwesampula Ye-Macro & Micro-Test,Ikhithi Yokuthumela Nokuthumela (HWTS-3002) yiJiangsu Macro kanye neMicro-Test Med-TechI-Co., Ltd.
Izinto ezisetshenziswayo zidinga kodwa azinikezwa:Amathiphu angenawo i-DNase/RNase, amagilavu angasetshenziswa,Amashubhu e-EP angenayo i-DNase/RNase.
