I-Influenza B Virus Nucleic Acid

Isithombe Esifakiwe Se-Influenza B Virus Nucleic Acid

Incazelo emfushane:

Le khithi yenzelwe ukuthola i-in vitro qualitative virus nucleic acid kumasampula e-nasopharyngeal kanye ne-oropharyngeal swab.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-RT127A-Influenza B Virus (Ukukhulisa I-Enzymatic Probe Isothermal)

Isitifiketi

I-Epidemiology

Igciwane lomkhuhlane, uhlobo olumele i-Orthomyxoviridae, liyigciwane elisongela impilo yabantu kakhulu futhi lingathelela kakhulu abantu abazolithwala. Ubhubhane lwezinkathi zonyaka luthelela abantu ababalelwa ezigidini ezingu-600 emhlabeni jikelele futhi lubangela ukufa kwabantu abangu-250,000 kuya ku-500,000 minyaka yonke, lapho igciwane lomkhuhlane B lingenye yezimbangela eziyinhloko.^[1]Igciwane le-Influenza B, elaziwa nangokuthi i-IVB, liyi-RNA ene-negative ene-strand eyodwa. Ngokusho kochungechunge lwe-nucleotide lwesifunda sayo se-HA1 esinezici ze-antigen, lingahlukaniswa ngezigaba ezimbili ezinkulu, izinhlobo ezimele yi-B/Yamagata/16/88 kanye ne-B/Victoria /2/87(5)^[2]. Igciwane le-Influenza B ngokuvamile linamandla kakhulu ekuqondeni umninimzi. Kutholakale ukuthi i-IVB ingathelela abantu nezilwane zasolwandle kuphela, futhi ngokuvamile ayibangeli ubhubhane womhlaba wonke, kodwa ingabangela ubhubhane lwezinkathi zesifunda.^[3]Igciwane le-Influenza B lingadluliselwa ngezindlela ezahlukene njengomgudu wokugaya ukudla, umgudu wokuphefumula, umonakalo wesikhumba kanye ne-conjunctiva. Izimpawu ikakhulukazi umkhuhlane ophezulu, ukukhwehlela, ikhala eligobhozayo, i-myalgia, njll. Iningi lazo lihambisana ne-pneumonia enzima, ukuhlaselwa yinhliziyo okukhulu. Ezimweni ezimbi kakhulu, ukwehluleka kwenhliziyo, izinso kanye nezinye izitho kuholela ekufeni, futhi izinga lokufa liphezulu kakhulu.^[4]Ngakho-ke, kunesidingo esiphuthumayo sendlela elula, enembile futhi esheshayo yokuthola igciwane le-influenza B, enganikeza isiqondiso semithi yokwelapha kanye nokuxilongwa.

Isiteshi

I-FAM	I-IVB nucleic acid
I-ROX	Ukulawula Kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	Uketshezi: ≤-18℃ Ebumnyameni Ukwenziwa kwe-Lyophilization: ≤30℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Uketshezi: izinyanga ezingu-9 Ukuvuselelwa kwe-Lyophilization: izinyanga ezingu-12
Uhlobo Lwesifanekiso	Amasampula e-nasopharyngeal swab Amasampula e-oropharyngeal swab
CV	≤10.0%
Tt	≤40
I-LoD	Ikhophi e-1/µL
Ukucaciswa	akukho ukusabela okuhlangene ne-Influenza A, i-Staphylococcus aureus,I-Streptococcus (kufaka phakathi i-Streptococcus pneumoniae), i-Adenovirus, i-Mycoplasma pneumoniae, i-Respiratory Syncytial Virus, i-Mycobacterium tuberculosis, i-Masles, i-Haemophilus influenzae, i-Rhinovirus, i-Coronavirus, i-Enteric Virus, i-swab yomuntu ophilile.
Izinsimbi Ezisebenzayo:	Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-SLAN ® -96P Zesikhathi Sangempela Uhlelo lwe-PCR lwe-LightCycler® 480 lwesikhathi sangempela Uhlelo Lokuthola Isothermal lwe-Easy Amp Real-time Fluorescence （HWTS1600）

Ukugeleza Komsebenzi

Inketho 1.

I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Inketho 2.

I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co.,Ltd.

Okwedlule: I-Influenza A Virus Nucleic Acid

Olandelayo: I-Mycoplasma Pneumoniae Nucleic Acid

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