I-Influenza B Virus Nucleic Acid

Isithombe Esifakiwe Se-Influenza B Virus Nucleic Acid

Incazelo emfushane:

Le khithi ihloselwe ukutholwa kwekhwalithi ye-in vitro virus ye-Influenza B nucleic acid kumasampuli we-nasopharyngeal kanye ne-oropharyngeal swab.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-RT127A-Influenza B Virus Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)

I-HWTS-RT128A-Ikhithi Yokuthola Igciwane Le-Nucleic Acid Eyomisiwe I-HWTS-RT128A(Enzymatic Probe Isothermal Amplification)

Isitifiketi

I-Epidemiology

Igciwane lomkhuhlane, uhlobo olumele i-Orthomyxoviridae, liyigciwane elisongela kakhulu impilo yabantu futhi lingathelela abantu abaningi.Izifo zomkhuhlane wezikhathi zonyaka zithelela abantu ababalelwa ezigidini ezingama-600 emhlabeni wonke futhi zibangela ukufa kwabantu abangu-250,000 kuya ku-500,000 minyaka yonke, okuyigciwane lomkhuhlane u-B ongenye yezimbangela ezinkulu.^[1].Igciwane loMkhuhlane B, elaziwa nangokuthi i-IVB, i-RNA enomucu owodwa onegayo.Ngokulandelana kwe-nucleotide yesifunda sayo se-HA1 ye-antigenic, ingahlukaniswa ngemigqa emibili emikhulu, izinhlobo ezimele i-B/Yamagata/16/88 kanye ne-B/Victoria /2/87(5)^[2].Igciwane lomkhuhlane B ngokuvamile linokucaciswa okuqinile kosokhaya.Kutholwe ukuthi i-IVB ingathelela abantu kuphela kanye nezimpawu, futhi ngokuvamile ayibangeli ubhubhane lomhlaba wonke, kodwa ingabangela izifo eziwubhubhane zesizini.^[3].Igciwane lomkhuhlane B lingasakazwa ngezindlela ezihlukahlukene ezifana nomgudu wokugaya ukudla, umgudu wokuphefumula, ukulimala kwesikhumba kanye ne-conjunctiva.Izimpawu ngokuyinhloko imfiva ephezulu, ukukhwehlela, ikhala eligijimayo, i-myalgia, njll. Iningi lazo lihambisana nenyumoniya enzima, ukuhlaselwa yinhliziyo okukhulu.Ezimweni ezimbi kakhulu, ukwehluleka kwenhliziyo, izinso nezinye izitho kuholela ekufeni, futhi izinga lokufa liphezulu kakhulu^[4].Ngakho-ke, kunesidingo esiphuthumayo sendlela elula, enembile futhi esheshayo yokuthola igciwane lomkhuhlane B, elinganikeza isiqondiso semithi yokwelapha kanye nokuxilongwa.

Isiteshi

FAM	I-IVB nucleic acid
I-ROX	Ukulawula Kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji	Uketshezi: ≤-18℃ ebumnyameni I-Lyophilization: ≤30℃ ebumnyameni
Impilo yeshelufu	Uketshezi: izinyanga ezingu-9 I-Lyophilization: izinyanga eziyi-12
Uhlobo lwesifanekiso	Amasampula e-Nasopharyngeal swab Amasampula e-Oropharyngeal swab
CV	≤10.0%
Tt	≤40
LoD	1 Ikhophi/µL
Ukucaciswa	akukho-reactivity cross-reactivity with Influenza A, Staphylococcus aureus,Streptococcus (kuhlanganise Streptococcus pneumoniae), Adenovirus, Mycoplasma pneumoniae, Respiratory Syncytial Virus, Mycobacterium tuberculosis, Isimungumungwane, Haemophilus influenzae, Rhinovirus, Coronavirus, Enteric Virus, swab yomuntu onempilo.
Izinsimbi Ezisebenzayo:	I-Applied Biosystems 7500 Real-Time PCR Systems I-SLAN ® -96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler® 480 Real-Time I-Easy Amp Real-time Fluorescence Isothermal Detection System (HWTS1600)

Ukugeleza komsebenzi

Inketho 1.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).

Inketho yesi-2.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: Isizinda se-Nucleic Acid noma i-Purification Reagent(YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.

Okwedlule: Umkhuhlane A Virus Nucleic Acid

Olandelayo: I-Human Respiratory Syncytial Virus Nucleic Acid

Bhala umyalezo wakho lapha futhi usithumelele wona