I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii kanye ne-Pseudomonas Aeruginosa kanye ne-Drug Resistance Genes (i-KPC, i-NDM, i-OXA48 kanye ne-IMP) Multiplex
Igama lomkhiqizo
I-HWTS-RT109 Klebsiella Pneumoniae, i-Acinetobacter Baumannii kanye ne-Pseudomonas Aeruginosa kanye ne-Drug Resistance Genes (i-KPC, i-NDM, i-OXA48 kanye ne-IMP) Multiplex Detection Kit (i-Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Klebsiella pneumoniae iyi-pathogen ejwayelekile yezokwelapha enethuba futhi ingenye yamagciwane abalulekile abangela izifo ezibangelwa yi-nosocomial. Lapho ukumelana komzimba kuncipha, amagciwane angena emaphashini esuka endleleni yokuphefumula, abangele ukutheleleka ezingxenyeni eziningi zomzimba, futhi ukusetshenziswa kwasekuqaleni kwama-antibiotic kuyisihluthulelo sokwelapha [1]. Indawo evame kakhulu yokutheleleka kwe-Acinetobacter baumannii yi-pathogen, okuyi-pathogen ebalulekile ye-Hospital acquired pneumonia (HAP), ikakhulukazi i-Ventilator associated pneumonia (VAP). Ivame ukuhambisana nezinye izifo ezibangelwa yi-bacterial kanye ne-fungal, ezinezici zesilinganiso esiphezulu sokugula kanye nesilinganiso esiphezulu sokufa. I-Pseudomonas aeruginosa iyi-bacilli engavamile kakhulu ye-gram-negative engeyona i-fermentative emtholampilo, futhi iyi-pathogen ebalulekile enethuba yokutheleleka okutholakala esibhedlela, enezici zokungena kalula ekoloni, ukuhlukahluka okulula kanye nokumelana nemithi eminingi.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela |
| Ct | ≤36 |
| CV | ≤5.0% |
| I-LoD | Amakhophi ayi-1000/mL |
| Ukucaciswa | a) Ukuhlolwa kokusabela okuxubile kukhombisa ukuthi le khithi ayinayo i-reactivity exubile nezinye izifo zokuphefumula, njenge-Streptococcus pneumoniae, i-Neisseria meningitidis, i-Staphylococcus aureus, i-Klebsiella oxytoca, i-Haemophilus influenzae, i-Acinetobacter jelly, i-Acinetobacter hemolytica, i-Legionella pneumophila, i-Escherichia coli, i-Pseudomonas fluorescens, i-Candida albicans, i-Chlamydia pneumoniae, i-Respiratory Adenovirus, amasampula e-Enterococcus kanye ne-sputum angenazo izinjongo, njll. b) Amandla okulwa nokuphazamiseka: Khetha i-mucin, i-minocycline, i-gentamicin, i-clindamycin, i-imipenem, i-cefoperazone, i-meropenem, i-ciprofloxacin hydrochloride, i-levofloxacin, i-clavulanic acid, kanye ne-roxithromycin, njll. ukuze kuhlolwe ukuphazamiseka, futhi imiphumela ikhombisa ukuthi izinto zokuphazamiseka okukhulunywe ngazo ngenhla aziphazamisi ukutholakala kwe-Klebsiella pneumoniae, i-Acinetobacter baumannii, i-Pseudomonas aeruginosa kanye ne-carbapenem resistance genes KPC, NDM, OXA48 kanye ne-IMP. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480, Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. iyanconywa ekukhishweni kwesampula futhi izinyathelo ezilandelayo kufanele zenziwe ngokuhambisana ne-IFU ye-Kit.
