I-Klebsiella Pneumoniae, i-Acinetobacter Baumannii ne-Pseudomonas Aeruginosa kanye Nezakhi Zofuzo Zokumelana Nezidakamizwa (KPC, NDM, OXA48 kanye ne-IMP) Multiplex
Igama lomkhiqizo
I-HWTS-RT109 Klebsiella Pneumoniae, i-Acinetobacter Baumannii ne-Pseudomonas Aeruginosa kanye Nezakhi Zofuzo Ezimelana Nezidakamizwa (KPC, NDM, OXA48 kanye ne-IMP) Ikhithi Yokuthola I-Multiplex(Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Klebsiella pneumoniae iyisifo esivamile somtholampilo esingosomathuba futhi ingelinye lamabhaktheriya abalulekile e-pathogenic abangela izifo ze-nosocomial. Lapho ukumelana komzimba kuncipha, amabhaktheriya angena emaphashini esuka emgudwini wokuphefumula, okubangela ukutheleleka ezingxenyeni eziningi zomzimba, futhi ukusetshenziswa kwangaphambi kwesikhathi kwemithi elwa namagciwane kuyisihluthulelo sokwelapha [1] .Indawo evamile yokutheleleka kwe-Acinetobacter baumannii ngamaphaphu, okuyi-pathogen ebalulekile ye-Hospital gained pneumonia (HAP), ikakhulukazi i-Ventilator ehambisana nenyumoniya (VAP). Ivamise ukuhambisana nezinye izifo ezibangelwa amagciwane kanye nesikhunta, ezinezici zezinga eliphezulu lokugula kanye nezinga eliphezulu lokufa kwabantu.I-Pseudomonas aeruginosa iyigciwane elivame kakhulu elingavundisi igram-negative emisebenzini yomtholampilo, futhi liyi-pathogen ebalulekile engosomathuba ekuthelelekeni okutholwa esibhedlela, enezici zokululamisa ikoloni, ukumelana nezidakamizwa eziningi.
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 amakhophi/mL |
| Ukucaciswa | a) Ukuhlolwa kwe-cross-reactivity kubonisa ukuthi le khithi ayinakho ukuphinda isebenze kabusha namanye amagciwane okuphefumula, njenge-Streptococcus pneumoniae, i-Neisseria meningitidis, i-Staphylococcus aureus, i-Klebsiella oxytoca, i-Haemophilus influenzae, i-Acinetobacter jelly, i-Acinetobacter, i-Leecimonelliephila, i-Epilalytics, i-Elenetobacter, i-Electer hemophilia I-Pseudomonas fluorescens, i-Candida albicans, i-Chlamydia pneumoniae, i-Respiratory Adenovirus, i-Enterococcus namasampula esikhwehlela ngaphandle kokuhlosiwe, njll. b)Amandla okunqanda ukuphazamiseka: Khetha i-mucin, i-minocycline, i-gentamicin, i-clindamycin, imipenem, i-cefoperazone, i-meropenem, i-ciprofloxacin hydrochloride, i-levofloxacin, i-clavulanic acid, ne-roxithromycin, njll. ngokuhlolwa kokuphazanyiswa, futhi imiphumela ibonisa ukuthi izinto zokuphazamisa ezishiwo ngenhla aziphazamisi i-K. i-baumannii, i-Pseudomonas aeruginosa kanye nezakhi zofuzo zokumelana ne-carbapenem i-KPC, i-NDM, i-OXA48 ne-IMP. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, I-QuantStudio®5 Amasistimu e-PCR wesikhathi sangempela, I-LightCycler®480 uhlelo lwe-PCR lwesikhathi sangempela, I-LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ubuchwepheshe be-Bioer), I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), I-BioRad CFX96 Real-Time PCR System, I-BioRad CFX Opus 96 Real-Time PCR System. |
Ukugeleza komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. IFU yekhithi.
