I-CYP2C9 yomuntu kanye ne-VKORC1 Gene Polymorphism

Incazelo emfushane:

Le khithi isetshenziswa ekutholweni kwekhwalithi ye-in vitro ye-polymorphism ye-CYP2C9*3 (rs1057910, 1075A>C) kanye ne-VKORC1 (rs9923231, -1639G>A) ku-DNA ye-genomic yamasampuli egazi lonke lomuntu.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-GE014A-Human CYP2C9 kanye ne-VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)

Isitifiketi

CE/TFDA

I-Epidemiology

I-Warfarin iyi-anticoagulant yomlomo evame ukusetshenziswa emisebenzini yomtholampilo njengamanje, ehloselwe kakhulu ukuvimbela nokwelashwa kwezifo ze-thromboembolic.Kodwa-ke, i-warfarin inefasitela lokwelapha elilinganiselwe futhi ihluka kakhulu phakathi kwezinhlanga ezahlukene kanye nabantu ngabanye.Izibalo zibonise ukuthi umehluko wethamo elizinzile kubantu abahlukene ungaba izikhathi ezingaphezu kwezingu-20.Ukopha okungathandeki kwenzeka ku-15.2% weziguli ezithatha i-warfarin unyaka ngamunye, lapho u-3.5% wazo ziba nokopha okubulalayo.Ucwaningo lwe-pharmacogenomic lubonise ukuthi i-polymorphism yofuzo ye-enzyme eqondiwe i-VKORC1 kanye ne-enzyme ye-metabolic CYP2C9 ye-warfarin iyisici esibalulekile esithinta umehluko kumthamo we-warfarin.I-Warfarin iyisivimbeli esithile se-vitamin K epoxide reductase (VKORC1), futhi ngaleyo ndlela ivimbela ukuhlanganiswa kwezinto ezijiyisayo ezihlanganisa uvithamini K futhi inikeza i-anticoagulation.Inani elikhulu locwaningo libonise ukuthi i-polymorphism yofuzo yomgqugquzeli we-VKORC1 iyisici esibaluleke kakhulu esithinta umjaho nokuhluka komuntu ngamunye kumthamo odingekayo we-warfarin.I-Warfarin yenziwa i-CYP2C9, futhi izinguquko zayo zehlisa kakhulu imetabolism ye-warfarin.Abantu abasebenzisa i-warfarin basengozini enkulu (ephindwe kabili ukuya kathathu) yokopha ekuqaleni kokusetshenziswa.

Isiteshi

FAM I-VKORC1 (-1639G>A)
CY5 I-CYP2C9*3
VIC/HEX IC

Imingcele Yezobuchwepheshe

Isitoreji Uketshezi: ≤-18℃
Impilo yeshelufu Izinyanga ezingu-12
Uhlobo lwesifanekiso Igazi elisha le-EDTA elingenayo i-anticoagulated
CV ≤5.0%
LoD 1.0ng/μL
Ukucaciswa Akukho ukuhlangana kabusha nokunye ukulandelana okungaguquki kakhulu kwe-genome yomuntu (ufuzo lwe-CYP2C19 yomuntu, isakhi sofuzo se-RPN2 yomuntu);ukuguqulwa kwe-CYP2C9*13 kanye ne-VKORC1 (3730G>A) ngaphandle kwebanga lokutholwa kwale khithi
Izinsimbi Ezisebenzayo I-Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

I-QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

I-LineGene 9600 Plus Real-Time PCR Detection System

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).


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