Isampula yokukhishwa kwe-Reagent
Igama lomkhiqizo
I-Macro & Micro-Test Release Sample Release Reagent
Isitifiketi
CE, FDA, NMPA
Izingxenye eziyinhloko
| Igama | Izingxenye eziyinhloko | Isakhiimininingwane | Ubuningi |
| Ukukhishwa Kwesampulai-reagent | I-Dithiothreitol, i-sodium dodecyli-sulfate (SDS), i-RNase inhibitor,surfactant, amanzi ahlanzekile | 0.5mL/Ibhodlela | 50 Isitsha |
Qaphela: Izingxenye kumaqoqo ahlukene wekhithi azishintsheki.
Izimo zesitoreji nempilo yeshalofu
Gcina futhi uhambise ekamelweni lokushisa.Isikhathi seshelufu yizinyanga ezingama-24.
Izinsimbi ezisebenzayo
Izinsimbi kanye nemishini ngesikhathi sokucubungula isampula, njengama-pipettes, ama-vortex mixers,okugeza amanzi, njll.
Izidingo zesampula
Amaswabhu e-oropharyngeal asanda kuqoqwa, ama-nasopharyngeal swabs.
Ukunemba
Uma le khithi isetshenziselwa ukukhipha ku-CV yereferensi yokunemba yangaphakathi kwezimpinda ezingu-10, i-coefficient of variation (CV, %) yenani le-Ct ayidluli u-10%.
Umehluko we-inter-batch
Lapho ireferensi yokunemba yangaphakathi ihlolwa kumaqoqo amathathu ekhithi ngaphansi kokukhiqizwa kwesilingo lapho ikhishwa ngokuphindaphindiwe futhi, i-coefficient of variation (CV, %) yenani le-Ct ayikho ngaphezu kuka-10%.
Ukuqhathaniswa kokusebenza
● Ukuqhathanisa ukusebenza kahle kwesizinda
| Ukuqhathaniswa okusebenzayo kwendlela yobuhlalu kazibuthe nesikhiphi sesampula | ||||
| ukugxilisa ingqondo | indlela yobuhlalu kazibuthe | umkhiphi wesampula | ||
| i-orfab | N | i-orfab | N | |
| 20000 | 28.01 | 28.76 | 28.6 | 29.15 |
| 2000 | 31.53 | 31.9 | 32.35 | 32.37 |
| 500 | 33.8 | 34 | 35.25 | 35.9 |
| 200 | 35.25 | 35.9 | 35.83 | 35.96 |
| 100 | 36.99 | 37.7 | 38.13 | undet |
Ukusebenza kahle kokukhipha isikhiphi sesampula kwakufana nendlela yobuhlalu kazibuthe, futhi ukugcwala kwe-pathogen kungaba ngu-200Copies/mL.
● Ukuqhathaniswa kwenani le-CV
| Ukuphindaphinda kokukhipha isampula yesikhiphi | ||
| ukugxila:5000Amakhophi/mL | ORF1ab | N |
| 30.17 | 30.38 | |
| 30.09 | 30.36 | |
| 30.36 | 30.26 | |
| 30.03 | 30.48 | |
| 30.14 | 30.45 | |
| 30.31 | 30.16 | |
| 30.38 | 30.7 | |
| 30.72 | 30.79 | |
| CV | 0.73% | 0.69% |
Lapho ihlolwa kumakhophi angu-5,000 /mL, i-CV ye-orFab ne-N yayingu-0.73% kanye no-0.69%, ngokulandelana.
