I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid
Igama lomkhiqizo
I-HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)
I-Epidemiology
I-Middle East Respiratory Syndrome coronavirus (MERS-CoV), i-β-coronavirus ebangelaisifo sokuphefumula kubantu, saqala ukukhonjwa esigulini esineminyaka engu-60 sase-Saudi Arabia esishonile ngomhla ka-24th July, 2012. Isethulo somtholampilo sokutheleleka kwe-MERS-CoV sisukela esimweni esingabonakali noma izimpawu zokuphefumula ezincane kuya ezifweni ezinzima zokuphefumula ngisho nokufa.
Isiteshi
FAM | Igciwane le-MERS RNA |
I-VIC(HEX) | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
Isitoreji | ≤-18℃ ebumnyameni |
Impilo yeshelufu | 9 izinyanga |
Uhlobo lwesifanekiso | Ama-swabs e-nasopharyngeal asanda kuqoqwa |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1000 Amakhophi/mL |
Ukucaciswa | Akukho ukuhlangana kabusha nama-coronavirus abantu abantu i-SARSr-CoV namanye amagciwane ajwayelekile. |
Izinsimbi Ezisebenzayo: | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokumba esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).