I-Middle East Respiratory Syndrome CORONAVIRUS NUCLEIC ACE
Igama lomkhiqizo
I-HWTS-RT031A-Middle East Respiratory Syndrome coronavirus nucleic acid detection detection (Fluorescent PCR)
I-Epidemiology
I-Middle East Respiratory Syndrome CORONAVIRUS (MERS-CoV), i-β-coronavirus ebangelaIsifo sokuphefumula kubantu, saqala sakhonjwa esigulini sesibeletho saseSaudi Arabia esineminyaka engama-24 ubudala esingu-24 kuJulayi, 2012
Insungubezi
-Yenza | I-Mers Virus RNNA |
I-VIC (Hex) | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
Ukubeka | ≤-18 ℃ ebumnyameni |
Ishalofu-impilo | Izinyanga eziyi-9 |
Uhlobo lwe-Specimen | Aqoqwe kabusha ama-nasopharyngell swabs |
CV | ≤5.0% |
Ct | ≤38 |
Indlu edayisa insimbi | Amakhophi ayi-1000 / ml |
Ukucacisa | Akukho okuphambana nokusebenzisa imali ngama-coronavirises womuntu we-coronavirus sarsr-cova kanye namanye amagciwane ajwayelekile. |
Izinsimbi ezisebenzayo: | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-realent enconyiwe I-realent: I-QAAMP Viral RNA MINI KIT (52904), isizinda se-nucleic acid noma i-realegent yokuhlanza (ypp15-R) nguTiangen Biotech (Beijing) Co, Ltd.
Inketho 2.
I-realent enconyiwe I-realent: Macro & Micro-Test General DNA / RNA KIT (HWTS-3017) kanye ne-macro & micro-aving automatic extractor (HWS-3006c, HWS-3006C).