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    • I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid

    I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Isithombe Esifakiwe
    • I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid

    Incazelo emfushane:

    Le khithi isetshenziselwa ukuthola i-nucleic acid ye-MERS coronavirus kuma-nasopharyngeal swabs ane-Middle East Respiratory Syndrome (MERS) coronavirus.


    Imininingwane Yomkhiqizo

    Amathegi Omkhiqizo

    Igama lomkhiqizo

    I-HWTS-RT031A-I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit (i-Fluorescent PCR)

    I-Epidemiology

    I-Middle East respiratory syndrome coronavirus (MERS-CoV), igciwane le-β-coronavirus elibangelaisifo sokuphefumula kubantu, saqala ukutholakala esigulini sesilisa saseSaudi Arabia esineminyaka engu-60 ubudala esishonile ngomhlaka-24 kuJulayi, 2012. Ukuvezwa kwezokwelapha kokutheleleka kwe-MERS-CoV kusuka esimweni sokungabonakali noma izimpawu zokuphefumula ezincane kuya esifweni sokuphefumula esibi kakhulu ngisho nokufa.

    Isiteshi

    I-FAM I-RNA yegciwane le-MERS
    I-VIC(HEX)

    Ukulawula Kwangaphakathi

    Amapharamitha Obuchwepheshe

    Isitoreji

    ≤-18℃ Ebumnyameni

    Isikhathi sokuphelelwa yisikhathi

    Izinyanga ezingu-9

    Uhlobo Lwesifanekiso

    Ama-nasopharyngeal swabs asanda kuqoqwa

    CV

    ≤5.0%

    Ct

    ≤38

    I-LoD

    		 Amakhophi ayi-1000/mL

    Ukucaciswa

    Akukho ukuhlangana kwe-coronavirus yabantu i-coronavirus yabantu i-SARSr-CoV kanye nezinye izifo ezivamile.

    Izinsimbi Ezisebenzayo:

    		 Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time

    Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time

    Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela

    Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P

    Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

    Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR

    I-MA-6000 Real-Time Quantitative Thermal Cycler

    Uhlelo lwe-BioRad CFX96 Real-Time PCR

    Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR

    Ukugeleza Komsebenzi

    Inketho 1.

    I-reagent yokukhipha enconyiwe: I-QIAamp Viral RNA Mini Kit (52904), I-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.

    Inketho 2.

    I-reagent yokukhipha enconywayo: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

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