I-Monkeypox Virus kanye nokuthayipha i-nucleic acid

I-Monkeypox Virus kanye nokuthayipha isithombe esifakiwe se-nucleic acid

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwe-virus virus ye-vitro ye-monkeypox virus clade i, clade II kanye ne-monkey virus virus Universal Nucleic Acids ku-Human Rash fluid, ama-oropharyngeal swabs kanye namasampula e-serum.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Igciwane le-HWTS-OT202-Monke02-Monkeype kanye nokuthayipha Kit Dection Detecting (Fluorescence PCR)

Isitifiketi

I-Epidemiology

I-Mokeypox (MPOX) yisifo esibi kakhulu se-zoolotic esithathelwanayo esidalwa yi-monkeyy virus (MPXV). I-MPXV iyindilinga eyindilinga noma i-oval ngesimo, futhi iyi-virus ekhuphuke kabili ye-DNA enobude be-197KB^[1]. Lesi sifo sidluliselwa ikakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa yizilwane ezinegciwane noma ngokuxhumana ngqo negazi, uketshezi lomzimba nokuqhuma kwezilwane ezinegciwane. Leli gciwane lingadluliselwa futhi phakathi kwabantu, ngokuyinhloko ngokusebenzisa amaconsi okuphefumula ngesikhathi sokuxoxa isikhathi eside, ngqo, ngokuxhumana nobuso noma ngokuxhumana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile^[2-3]. Ucwaningo luye lwabonisa ukuthi i-MPXV yakha ama-Clades amabili ahlukene: I-Clade I (phambilini yaziwa ngokuthi yiCentral African Basin Clade) kanye neClade II (phambilini ibizwa ngeNtshonalanga Afrika Clade). I-MPOX yeCongo Basin Clade iboniswe ngokucacile ukuthi ingathathwa phakathi kwabantu futhi ingadala ukufa, kanti i-MPOX ye-MPER CLAFE CLADE ibangela izimpawu eziphansi futhi inesilinganiso esiphansi sokudluliselwa komuntu^[4].

Imiphumela yokuhlolwa kwale khithi ayihloselwe ukuba yinkomba kuphela yokuthola ukutheleleka nge-MPXV ezigulini, okumele ihlanganiswe nezimpawu zomtholampilo zesiguli kanye neminye imininingwane yokuhlolwa kwelabhoratri ukwenza kahle ukwelashwa okunengqondo Hlela ukuqinisekisa ukwelashwa okuphephile nokusebenzayo.

Insungubezi

-Yenza	I-MPXV Clade II
Ivupho	I-MPXV Universal nucleic acid
VIC / Hex	I-MPXV ye-Clade i
Cy5	Ukulawulwa Kwangaphakathi

Amapharamitha Ezobuchwepheshe

Ukubeka	≤-18 ℃
Ishalofu-impilo	Izinyanga eziyi-12
Uhlobo lwe-Specimen	uketshezi lwama-rash ongumuntu, ama-oropharyngex swabs kanye ne-serum
Ct	≤38 (Fam, Vic / Hex, Rox), ≤35 (IC)
Indlu edayisa insimbi	Amakhophi angama-200 / ml
Izinsimbi ezisebenzayo	Uhlobo I Recegent realent: Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio^®Amasistimu we-PCR e-Real-time real ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler^®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 kanye nezinhlelo zokutholwa kwe-PRCR yesikhathi sangempela (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR Uhlobo II Detection realent: -Nodoku nawo^TMI-AIO800 (HWS-EQ007)) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd.