I-Monkeypox Virus kanye ne-Typing Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukutholwa kwekhwalithi ye-in vitro virus yemonkeypox clade I, i-clade II kanye ne-monkeypox virus universal nucleic acids kuketshezi lwama-human rash, ama-oropharyngeal swabs kanye namasampula e-serum.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-OT202-Monkeypox Virus kanye ne-Typing Nucleic Acid Detection Kit (Fluorescence PCR)

Isitifiketi

I-Epidemiology

I-Monkeypox (i-Mpox) isifo esithathelwanayo se-zoonotic esibangelwa i-Monkeypox Virus (MPXV). I-MPXV iyizitini eziyindilinga noma imise okweqanda, futhi iyigciwane le-DNA elinemicu emibili elinobude obucishe bube ngu-197Kb.^[1]. Lesi sifo sithathelwana kakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa izilwane ezinalesi sifo noma ngokuthintana ngqo negazi, uketshezi oluvela emzimbeni kanye nokuqubuka kwezilwane ezinalesi sifo. Leli gciwane lingadluliselwa phakathi kwabantu, ikakhulukazi ngamaconsi okuphefumula ngesikhathi eside, sokuthintana ngqo ubuso nobuso noma ngokuthintana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile.^[2-3]. Ucwaningo luye lwabonisa ukuthi i-MPXV yakha izigaba ezimbili ezihlukene: i-clade I (phambilini eyayaziwa ngokuthi i-Central African clade noma i-Congo Basin clade) kanye ne-clade II (ngaphambili eyayibizwa ngokuthi i-West African clade). I-mpox ye-Congo Basin clade iboniswe ngokucacile ukuthi idluliselwa phakathi kwabantu futhi ingabangela ukufa, kuyilapho i-mpox ye-West African clade ibangela izimpawu ezithambile futhi inezinga eliphansi lokusulelana komuntu kuya kumuntu.^[4].

Imiphumela yokuhlolwa yale kit ayihloselwe ukuba ibe inkomba yodwa yokuhlonza ukutheleleka kwe-MPXV ezigulini, okumele kuhlanganiswe nezici zomtholampilo zesiguli kanye nenye idatha yokuhlolwa kwelabhorethri ukuze kwahlulele kahle ukutheleleka kwe-pathogen futhi kwakhiwe uhlelo lokwelapha olunengqondo ukuze kuqinisekiswe ukwelashwa okuphephile nokuphumelelayo.

Isiteshi

FAM	I-MPXV clade II
I-ROX	MPXV universal nucleic acid
VIC/HEX	I-MPXV clade I
CY5	ukulawula kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji	≤-18℃
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	uketshezi lwe-human rash, i-oropharyngeal swabs kanye ne-serum
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35(IC)
LoD	200 amakhophi/mL
Izinsimbi Ezisebenzayo	Uhlobo I-reagent yokuthola: I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio^®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) I-LightCycler^®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 ye-Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System I-reagent yokuthola uhlobo II: Eudemon^TMI-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.