Igciwane leMonkeypox kanye neTyping Nucleic Acid

Isithombe Esifakiwe Segciwane Le-Monkeypox kanye Ne-Typing Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-in vitro qualitative nucleic acids yegciwane le-monkeypox i-clade I, i-clade II kanye negciwane le-monkeypox ku-human rash fluid, ama-oropharyngeal swabs kanye namasampula e-serum.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-OT202-Monkeypox Virus kanye ne-Typing Nucleic Acid Detection Kit (i-Fluorescence PCR)

Isitifiketi

I-Epidemiology

I-Monkeypox (i-Mpox) isifo esithathelwanayo esibangelwa yi-Monkeypox Virus (MPXV). I-MPXV inesimo esiyindilinga noma esiyisiyingi, futhi iyigciwane le-DNA elinezintambo ezimbili elinobude obungaba ngu-197Kb.^[1]Lesi sifo sidluliselwa kakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa yizilwane ezithelelekile noma ngokuthintana ngqo negazi, uketshezi lomzimba kanye nokuqubuka kwezilwane ezithelelekile. Leli gciwane lingadluliselwa phakathi kwabantu, ikakhulukazi ngamaconsi okuphefumula ngesikhathi sokuxhumana ubuso nobuso isikhathi eside, noma ngokuthintana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile.^[2-3]Ucwaningo luye lwabonisa ukuthi i-MPXV yakha ama-clade amabili ahlukene: i-clade I (eyayaziwa ngokuthi i-Central African clade noma i-Congo Basin clade) kanye ne-clade II (eyayibizwa ngokuthi i-West African clade). I-mpox ye-Congo Basin clade iboniswe ngokusobala ukuthi idluliselwa phakathi kwabantu futhi ingabangela ukufa, kuyilapho i-mpox ye-West African clade ibangela izimpawu ezincane futhi inezinga eliphansi lokudluliselwa komuntu nomuntu.^[4].

Imiphumela yokuhlolwa yale khithi ayihloselwe ukuba yinkomba yodwa yokutholakala kokutheleleka kwe-MPXV ezigulini, okumele kuhlanganiswe nezici zomtholampilo zesiguli kanye neminye imininingwane yokuhlolwa kwelabhorethri ukuze kwahlulelwe ngokufanele ukutheleleka kwegciwane futhi kwakhiwe uhlelo lokwelapha olufanele ukuqinisekisa ukwelashwa okuphephile nokuphumelelayo.

Isiteshi

I-FAM	Isigaba II se-MPXV
I-ROX	I-MPXV i-nucleic acid yonke
I-VIC/HEX	Isigaba se-MPXV I
CY5	ukulawula kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	≤-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	uketshezi lokuqubuka komuntu, ama-oropharyngeal swabs kanye ne-serum
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35（IC)
I-LoD	Amakhophi angu-200/mL
Izinsimbi Ezisebenzayo	I-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR I-reagent yokuthola uhlobo lwesibili: I-Eudemon^TMI-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.