I-Monkeypox Virus Nucleic Acid
Igama lomkhiqizo
I-HWTS-OT200 Monkeypox Virus Nucleic Acid Detection Kit(Enzymatic Probe Isothermal Amplification)
Isitifiketi
CE
I-Epidemiology
I-Monkeypox (MPX) yisifo esithathelwanayo se-zoonotic esibangelwa i-Monkeypox Virus (MPXV). I-MPXV iyindilinga eyenziwe ngezitini noma imise okweqanda, futhi iyigciwane le-DNA elinemicu ekabili elinobude obungaba ngu-197Kb. Lesi sifo sithathelwana kakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa izilwane ezinalesi sifo noma ngokuthintana ngqo negazi, uketshezi oluvela emzimbeni kanye nokuqubuka kwezilwane ezinalesi sifo. Leli gciwane lingadluliselwa phakathi kwabantu, ikakhulukazi ngamaconsi okuphefumula ngesikhathi eside, sokuthintana ngqo nobuso noma ngokuthintana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile. Izimpawu zomtholampilo zokutheleleka kwemonkeypox kubantu zifana nalezo zengxibongo, ngokuvamile ngemva kwezinsuku eziyi-12 zokufukamela, ukubonakala kwemfiva, ikhanda elibuhlungu, imisipha nomhlane, ama-lymph nodes akhulisiwe, ukukhathala nokungakhululeki. Ukuqhuma kubonakala ngemva kwezinsuku ezingu-1-3 zomkhuhlane, ngokuvamile kuqala ebusweni, kodwa nakwezinye izingxenye. Inkambo yesifo ngokuvamile ithatha amasonto angu-2-4, kanti izinga lokufa liyi-1% -10%. I-Lymphadenopathy ingenye yomehluko omkhulu phakathi kwalesi sifo kanye nengxibongo.
Imiphumela yokuhlolwa yale kit akufanele isetshenziswe njengenkomba yodwa yokuhlonza ukutheleleka ngegciwane lemonkeypox ezigulini, okumele kuhlanganiswe nezici zomtholampilo zesiguli kanye neminye imininingwane yokuhlola yaselabhorethri ukucacisa kahle ukutheleleka kwe-pathogen, futhi yenza uhlelo lokwelapha oluphusile ukuze wenze ukwelashwa kuphephe futhi kusebenze kahle.
Imingcele Yezobuchwepheshe
Uhlobo lwesifanekiso | uketshezi lwe-human rash, i-oropharyngeal swab |
Isiteshi | FAM |
Tt | 28 |
CV | ≤5.0% |
LoD | 200 Amakhophi/μL |
Ukucaciswa | Sebenzisa ikhithi ukuthola amanye amagciwane, njengegciwane leNgxibongo, igciwane le-Cowpox, igciwane leVaccinia,I-Herpes simplex virus, njll., futhi akukho ukusabela okuphambene. |
Izinsimbi Ezisebenzayo | I-Easy Amp Real-time Fluorescence Isothermal Detection System (HWTS 1600) I-Applied Biosystems 7500 Real-Time PCR Systems, I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR Systems, I-BioRad CFX Opus 96 Real-Time PCR Systems. |