I-Monkeypox Virus Nucleic Acid

Isithombe Esifakiwe Se-Monkeypox Virus Nucleic Acid

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola i-nucleic acid yegciwane le-monkeypox ngaphakathi kwesibeletho kumasampula oketshezi olunamaqhubu kanye ne-oropharyngeal swab.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola I-HWTS-OT200 Monkeypox Virus Nucleic Acid (Ukukhulisa I-Enzymatic Probe Isothermal)

Isitifiketi

I-Epidemiology

I-Monkeypox (MPX) isifo esithathelwanayo esibangelwa yi-Monkeypox Virus (MPXV). I-MPXV inesimo esiyindilinga noma esiyisiyingi, futhi iyigciwane le-DNA elinezintambo ezimbili elinobude obungaba ngu-197Kb. Lesi sifo sidluliselwa kakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa yizilwane ezithelelekile noma ngokuthintana ngqo negazi, uketshezi lomzimba kanye nokuqubuka kwezilwane ezithelelekile. Leli gciwane lingadluliselwa futhi phakathi kwabantu, ikakhulukazi ngamaconsi okuphefumula ngesikhathi sokuxhumana ubuso nobuso isikhathi eside, noma ngokuthintana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile. Izimpawu zomtholampilo zokutheleleka kwe-monkeypox kubantu zifana neze-smallpox, ngokuvamile ngemva kwesikhathi sokufukamela sezinsuku ezingu-12, kuvela umkhuhlane, ikhanda elibuhlungu, ubuhlungu bemisipha nomhlane, ama-lymph node akhuphukile, ukukhathala kanye nokungakhululeki. Ukuqubuka kuvela ngemva kwezinsuku ezingu-1-3 zomkhuhlane, ngokuvamile kuqala ebusweni, kodwa nakwezinye izingxenye. Inkambo yesifo ngokuvamile ihlala amasonto angu-2-4, kanti izinga lokufa lingu-1%-10%. I-Lymphadenopathy ingenye yezindlela eziyinhloko zokwehluka phakathi kwalesi sifo kanye ne-smallpox.

Imiphumela yokuhlolwa yale khithi akufanele isetshenziswe njengesibonakaliso sodwa sokutholakala kokutheleleka kwegciwane le-monkeypox ezigulini, okumele kuhlanganiswe nezici zomtholampilo zesiguli kanye neminye imininingwane yokuhlolwa kwelabhorethri ukuze kutholakale kahle ukutheleleka kwegciwane, futhi kwakhiwe uhlelo lokwelapha olufanele ukuze kwenziwe ukwelashwa kuphephe futhi kuphumelele.

Amapharamitha Obuchwepheshe

Uhlobo Lwesifanekiso	uketshezi lokuqubuka komuntu, i-oropharyngeal swab
Isiteshi	I-FAM
Tt	28
CV	≤5.0%
I-LoD	Amakhophi angu-200/μL
Ukucaciswa	Sebenzisa ikhithi ukuthola amanye amagciwane, njengegciwane le-Smallpox, igciwane le-Cowpox, igciwane le-Vaccinia,Igciwane le-Herpes simplex, njll., futhi akukho ukusabela okuxubile.
Izinsimbi Ezisebenzayo	Uhlelo Lokuthola Isothermal Lokukhanya Kwe-Easy Amp Real-time (HWTS 1600) Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Izinhlelo ze-BioRad CFX96 Real-Time PCR, Izinhlelo ze-BioRad CFX Opus 96 ze-Real-Time PCR.