I-Monkeypox Virus Typing Nucleic Acid
Igama lomkhiqizo
I-HWTS-OT201I-Monkeypox Virus Typing Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-Monkeypox (i-Mpox) isifo esithathelwanayo se-zoonotic esibangelwa i-Monkeypox Virus (MPXV). I-MPXV iyindilinga eyenziwe ngezitini noma imise okweqanda, futhi iyigciwane le-DNA elinemicu ekabili elinobude obungaba ngu-197Kb. Lesi sifo sithathelwana kakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa izilwane ezinalesi sifo noma ngokuthintana ngqo negazi, uketshezi oluvela emzimbeni kanye nokuqubuka kwezilwane ezinalesi sifo. Leli gciwane lingadluliselwa phakathi kwabantu, ikakhulukazi ngamaconsi okuphefumula ngesikhathi eside, sokuthintana ngqo nobuso noma ngokuthintana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile. Ucwaningo luye lwabonisa ukuthi i-MPXV yakha izigaba ezimbili ezihlukene: i-clade I (phambilini eyayaziwa ngokuthi i-Central African clade noma i-Congo Basin clade) kanye ne-clade II (ngaphambili eyayibizwa ngokuthi i-West African clade). I-mpox ye-Congo Basin clade iboniswe ngokucacile ukuthi idluliselwa phakathi kwabantu futhi ingabangela ukufa, kuyilapho i-mpox ye-West African clade ibangela izimpawu ezithambile futhi inezinga eliphansi lokusulelana komuntu kuya kumuntu.
Imiphumela yokuhlolwa yale kit ayihloselwe ukuba ibe inkomba yodwa yokuhlonza ukutheleleka kwe-MPXV ezigulini, okumele kuhlanganiswe nezici zomtholampilo zesiguli kanye nenye idatha yokuhlolwa kwelabhorethri ukuze kwahlulele kahle ukutheleleka kwe-pathogen futhi kwakhiwe uhlelo lokwelapha olunengqondo ukuze kuqinisekiswe ukwelashwa okuphephile nokuphumelelayo.
Imingcele Yezobuchwepheshe
| Uhlobo Lwesifanekiso | uketshezi lwe-human rash, i-oropharyngeal swab kanye ne-serum |
| Ct | 38 |
| FAM | FAM-MPXV clade II VIC/HEX-MPXV clade I |
| CV | ≤5.0% |
| LoD | 200 Amakhophi/μL |
| Ukucaciswa | Sebenzisa ikhithi ukuthola amanye amagciwane, njengegciwane leNgxibongo, igciwane le-Cowpox, igciwane leVaccinia, HSV1, HSV2, Human Herpesvirus type 6, Human Herpesvirus type 7, Human Herpesvirus type 8, Measels vieus, Chicken pox-Herpes zoster virus, EB virus, Rubella virus njll., kanye akukho ukusabela okuphambene. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR Systems I-BioRad CFX Opus 96 Real-Time PCR Systems |
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