I-monkeypox virus yokuthayipha i-nucleic acid
Igama lomkhiqizo
I-HWTS-OT201I-monkeypox virus typing kit yokutholwa kwe-nucleic acid(Fleorescence PCR)
I-Epidemiology
I-Mokeypox (MPOX) yisifo esibi kakhulu se-zoolotic esithathelwanayo esidalwa yi-monkeyy virus (MPXV). I-MPXV iyindilinga eyindilinga noma i-oval ngesimo, futhi iyi-virus ye-DNA ekhutshwe kabili ngobude obungaba ngu-197KB. Lesi sifo sidluliselwa ikakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa yizilwane ezinegciwane noma ngokuxhumana ngqo negazi, uketshezi lomzimba nokuqhuma kwezilwane ezinegciwane. Leli gciwane lingadluliselwa futhi phakathi kwabantu, ngokuyinhloko ngokusebenzisa amaconsi okuphefumula ngesikhathi sokuxoxa isikhathi eside, oqondile kobuso noma ngokuxhumana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile. Ucwaningo luye lwabonisa ukuthi i-MPXV yakha ama-Clades amabili ahlukene: I-Clade I (phambilini yaziwa ngokuthi yiCentral African Basin Clade) kanye neClade II (phambilini ibizwa ngeNtshonalanga Afrika Clade). I-MPOX yeCongo Basin Clade iboniswe ngokucacile ukuthi ingathathwa phakathi kwabantu futhi ingadala ukufa, kanti i-MPOX ye-MP African Africa Clade ibangela izimpawu eziphansi futhi inesilinganiso esiphansi sokudluliselwa komuntu.
Imiphumela yokuhlolwa kwale khithi ayihloselwe ukuba yinkomba kuphela yokuthola ukutheleleka nge-MPXV ezigulini, okumele ihlanganiswe nezimpawu zomtholampilo zesiguli kanye neminye imininingwane yokuhlolwa kwelabhoratri ukwenza kahle ukwelashwa okunengqondo Hlela ukuqinisekisa ukwelashwa okuphephile nokusebenzayo.
Amapharamitha Ezobuchwepheshe
Uhlobo lwe-Specimen | uketshezi lwamanzi lomuntu, i-oropharyngengeal swab ne-serum |
Ct | 38 |
-Yenza | Fam-Mpxvv Clade II VIC / Hex-Mpxv I-Clade i |
CV | ≤5.0% |
Indlu edayisa insimbi | Amakhophi angama-200 / μL |
Ukucacisa | Sebenzisa i-kit ukuthola amanye amagciwane, njengegciwane le-smongpox, i-cowpox virus, i-voccinia virus, I-HSV1, HSV2, Uhlobo lwe-Human Herpesvirus 6, Uhlobo lweHerpesvirus lomuntu 7, Human Herpesvirus Thayipha 8, ama-VIEELS VIEUS, Inkukhu Pox-Herpes Zoster Virus, i-EB Virus, i-rubella virus njll, futhi Akukho ukusabela kwesiphambano. |
Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-LineGene 9600 Plus Real-Time Pcc Retection Systems I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Systems real-time PCR I-Biorad CFX Opus 96 Systems real-time PCREMS |