Uhlobo lwe-Monkeypox Virus Nucleic Acid
Igama lomkhiqizo
I-HWTS-OT201Ikhithi Yokuthola I-Nucleic Acid Yokuthayipha Igciwane Le-Monkeypox(I-PCR ye-Fluorescence)
I-Epidemiology
I-Monkeypox (Mpox) isifo esithathelwanayo esibangelwa yi-Monkeypox Virus (MPXV). I-MPXV inesimo esiyindilinga noma esiyisiyingi, futhi iyigciwane le-DNA elinezintambo ezimbili elinobude obungaba ngu-197Kb. Lesi sifo sidluliselwa kakhulu yizilwane, futhi abantu bangatheleleka ngokulunywa yizilwane ezithelelekile noma ngokuthintana ngqo negazi, uketshezi lomzimba kanye nokuqubuka kwezilwane ezithelelekile. Leli gciwane lingadluliselwa futhi phakathi kwabantu, ikakhulukazi ngamaconsi okuphefumula ngesikhathi sokuxhumana ubuso nobuso isikhathi eside, noma ngokuthintana ngqo noketshezi lomzimba wesiguli noma izinto ezingcolile. Izifundo zikhombisile ukuthi i-MPXV yakha ama-clade amabili ahlukene: i-clade I (eyayaziwa ngokuthi i-Central African clade noma i-Congo Basin clade) kanye ne-clade II (eyayibizwa ngokuthi i-West African clade). I-mpox ye-Congo Basin clade ikhonjiswe ngokusobala ukuthi idluliselwa phakathi kwabantu futhi ingabangela ukufa, kuyilapho i-mpox ye-West African clade ibangela izimpawu ezincane futhi inezinga eliphansi lokudluliselwa komuntu nomuntu.
Imiphumela yokuhlolwa yale khithi ayihloselwe ukuba yinkomba yodwa yokutholakala kokutheleleka kwe-MPXV ezigulini, okumele kuhlanganiswe nezici zomtholampilo zesiguli kanye neminye imininingwane yokuhlolwa kwelabhorethri ukuze kwahlulelwe ngokufanele ukutheleleka kwegciwane futhi kwakhiwe uhlelo lokwelapha olufanele ukuqinisekisa ukwelashwa okuphephile nokuphumelelayo.
Amapharamitha Obuchwepheshe
| Uhlobo Lwesifanekiso | uketshezi lokuqubuka komuntu, i-oropharyngeal swab kanye ne-serum |
| Ct | 38 |
| I-FAM | Isigaba se-FAM-MPXV II Isigaba se-VIC/HEX-MPXV I |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-200/μL |
| Ukucaciswa | Sebenzisa ikhithi ukuthola amanye amagciwane, njengegciwane le-Smallpox, igciwane le-Cowpox, igciwane le-Vaccinia, I-HSV1, i-HSV2, i-Human Herpesvirus uhlobo 6, i-Human Herpesvirus uhlobo 7, i-Human Herpesvirus uhlobo 8, i-Measels vieus, i-Chicken pox-i-Herpes zoster virus, i-EB virus, i-Rubella virus njll., kanye akukho ukusabela okuphambene. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Izinhlelo ze-PCR ze-BioRad CFX96 Real-Time Izinhlelo ze-BioRad CFX Opus 96 ze-Real-Time PCR |
Ukugeleza Komsebenzi
