I-Mthth gene polymorphic nucleic acid
Igama lomkhiqizo
I-HWTS-GE004-MTHFR GENECE polymorphic nucleic acid detection Kit (Arms-PCR)
I-Epidemiology
I-Folic acid iyivithamini engenamanzi elingukuthi i-cofactor ebalulekile emikhondweni ye-metabolic yomzimba. Eminyakeni yamuva nje, inqwaba yezifundo eziningi ikuqinisekisile ukuthi, ukuguquka kwenguquko ye-enzyme gene gene gene gene kuzoholela ekusweleni kwe-folic acid emzimbeni, kanye nomonakalo ojwayelekile wokuntuleka kwe-folic acid kwabadala kungadala i-megaloblastic anemia, vascular Ukulimala kwe-Endothelial, njll. I-Folic acid Ukuntuleka kwabesifazane abakhulelwe awakwazi ukufeza izidingo zazo kanye nengane, engadala izinkinga ze-neural tube, i-anancephaly, ukuzala nokushiswa kwesisu. Amazinga we-Serum Foote athintwa yi-5,10-methylenetrahydrotahyddrote (Mthfr) ama-polymorphisms. I-677c> T ne-1298A> C i-eCenitions in the Mthfr gene gene donsela ukuguqulwa kwe-Alanine kuya ku-valine ne-glutamic acid umsebenzi we-nthfr futhi ngenxa yalokho anciphise ukusetshenziswa kwe-folic acid.
Insungubezi
| -Yenza | Mthfr c67t |
| Ivupho | Mthfr a1298c |
| I-VIC (Hex) | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Igazi elisha landa aqoqwa i-Anticouagulated |
| CV | ≤5.0% |
| Ct | ≤38 |
| Indlu edayisa insimbi | I-1.0NG / μL |
| Izinsimbi ezisebenzayo: | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR SLAN ®-96P real-time PCRS Systems I-QuantStudio ™ 5 Izinhlelo ze-PCR zangempela zesikhathi sangempela I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1
I-reagents yokukhishwa enconyiwe: I-Macro & Micro-Tray Genomic DNA KNA (HWTS-3014-32, HWS-3014-48, HWS-3014-46, I-MACLE & Micro-3006c, HWS-3006C) .
Inketho 2
I-reagents enconyelwe ukukhishwa: I-GNA Genomic DNA Extraction Kit (YDPS48, JCXB20210062) nguTiangen Biotech (Beijing) Co, Ltd. I-genome genome detection kit (A1120) nguPromega.
