I-MTHFR Gene Polymorphic Nucleic Acid
Igama lomkhiqizo
I-HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid Detection Kit (ARMS-PCR)
I-Epidemiology
I-Folic acid iyivithamini encibilika emanzini eyi-cofactor ebalulekile emigudwini ye-metabolic yomzimba.Eminyakeni yamuva nje, inani elikhulu locwaningo liqinisekisile ukuthi, ukuguqulwa kwe-folate metabolizing enzyme gene MTHFR kuzoholela ekuntulekeni kwe-folic acid emzimbeni, futhi umonakalo ovamile wokuntuleka kwe-folic acid kubantu abadala kungase kubangele i-megaloblastic anemia, i-vascular. ukulimala kwe-endothelial, njll. Ukuntuleka kwe-folic acid kwabesifazane abakhulelwe abakwazi ukuhlangabezana nezidingo zabo kanye ne-fetus, okungabangela ukukhubazeka kwe-neural tube, i-anencephaly, ukuzala, nokuphuphuma kwesisu.Amazinga we-serum folate athintwa yi-5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms.Ukuguqulwa kwe-677C>T kanye ne-1298A>C kufuzo lwe-MTHFR kudala ukuguqulwa kwe-alanine ku-valine ne-glutamic acid, ngokulandelana, okuholela ekunciphiseni komsebenzi we-MTHFR futhi ngenxa yalokho kunciphisa ukusetshenziswa kwe-folic acid.
Isiteshi
| FAM | I-MTHFR C677T |
| I-ROX | I-MTHFR A1298C |
| I-VIC(HEX) | Ukulawula kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | Igazi elisanda kuqoqwa i-EDTA anticoagulated |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Izinsimbi Ezisebenzayo: | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-SLAN ®-96P Real-Time PCR Systems I-QuantStudio™ 5 Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1
Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWTS-300) .
Inketho yesi-2
Ama-reagents okukhipha anconyiwe: Ikhithi yokukhipha i-Blood Genomic DNA(YDP348, JCXB20210062) yi-Tiangen Biotech(Beijing) Co.,Ltd.Ikhithi yokukhipha iGenome yegazi(A1120) nge-Promega.
