I-MTHFR Gene Polymorphic Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-GE004-MTHFR Gene Polymorphic Nucleic Acid (ARMS-PCR)
I-Epidemiology
I-Folic acid iyi-vithamini encibilika emanzini eyingxenye ebalulekile yezindlela zomzimba zokugaya ukudla. Eminyakeni yamuva nje, izifundo eziningi ziqinisekisile ukuthi, ukuguqulwa kwe-folate metabolizing enzyme gene MTHFR kuzoholela ekushodeni kwe-folic acid emzimbeni, futhi umonakalo ovamile wokuntuleka kwe-folic acid kubantu abadala ungabangela i-megaloblastic anemia, umonakalo we-vascular endothelial, njll. Ukuntuleka kwe-folic acid kwabesifazane abakhulelwe akukwazi ukuhlangabezana nezidingo zabo kanye nengane encane, okungabangela ukukhubazeka kwe-neural tube, i-anencephaly, ukuzalwa kwengane ifile, kanye nokuphuphuma kwesisu. Amazinga e-serum folate athintwa yi-5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms. Ukuguqulwa kwe-677C>T kanye ne-1298A>C ku-MTHFR gene kubangela ukuguqulwa kwe-alanine kube yi-valine kanye ne-glutamic acid, ngokulandelana, okuholela ekunciphiseni umsebenzi we-MTHFR futhi ngenxa yalokho kunciphisa ukusetshenziswa kwe-folic acid.
Isiteshi
| I-FAM | I-MTHFR C677T |
| I-ROX | I-MTHFR A1298C |
| I-VIC(HEX) | Ukulawula kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Igazi elisanda kuqoqwa le-EDTA elingavimbi igazi |
| CV | ≤5.0% |
| Ct | ≤38 |
| I-LoD | 1.0ng/μL |
| Izinsimbi Ezisebenzayo: | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-SLAN ®-96P Zesikhathi Sangempela Izinhlelo ze-PCR ze-QuantStudio™ ezi-5 zesikhathi sangempela Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1
Ama-reagent okukhipha anconyiwe: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Inketho 2
Ama-reagent okukhipha anconyiwe: I-Blood Genomic DNA Extraction Kit (YDP348, JCXB20210062) yi-Tiangen Biotech (Beijing) Co.,Ltd. I-Blood Genome Extraction Kit (A1120) yi-Promega.
