I-DNA ye-Mycobacterium Tuberculosis
Igama lomkhiqizo
Ikhithi Yokuthola I-DNA Yesifo Sofuba i-HWTS-RT001-Mycobacterium (i-PCR ye-Fluorescence)
Isitifiketi
CE
I-Epidemiology
I-Mycobacterium culosis ibizwa ngokuthi i-Tubercle bacillus (TB). I-Mycobacterium tuberculosis ebangela izifo kubantu manje ibhekwa njengeyezinhlobo zabantu, izinkomo, kanye nezase-Afrika. Ukubangela izifo kwayo kungase kuhlobane nokuvuvukala okubangelwa ukwanda kwamagciwane emangqamuzaneni ezicubu, ubuthi bezingxenye zamagciwane kanye nama-metabolites, kanye nomonakalo wokuzivikela komzimba ezingxenyeni zamagciwane. Izinto ezibangela izifo zihlotshaniswa nama-capsule, ama-lipid kanye namaprotheni.
Isifo sofuba se-Mycobacterium singahlasela izinto eziphilayo ezisengozini ngokusebenzisa indlela yokuphefumula, indlela yokugaya ukudla noma ukulimala kwesikhumba, okubangela isifo sofuba sezicubu nezitho ezahlukahlukene, okuvame kakhulu kuso isifo sofuba se-pulmonary ngokusebenzisa indlela yokuphefumula. Ngokuvamile sivela ezinganeni, futhi siveza izimpawu ezifana nomkhuhlane wezinga eliphansi, ukujuluka ebusuku, kanye nenani elincane le-hemoptysis. Ukutheleleka kwesibili kubonakala kakhulu njengomkhuhlane wezinga eliphansi, ukujuluka ebusuku, kanye ne-hemoptysis. Ngokuvamile yisifo esingamahlalakhona sesikhathi eside. Ngo-2018, abantu ababalelwa ezigidini eziyi-10 emhlabeni wonke batheleleka ngesifo sofuba se-Mycobacterium, okwathi cishe izigidi eziyi-1.6 zabo zafa.
Isiteshi
| I-FAM | I-DNA ye-nucleic acid ye-Target (IS6110 kanye ne-38KD) |
| I-VIC (HEX) | Ukulawula kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ Ebumnyameni; I-Lyophilized: ≤30℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isikhwehlela |
| Ct | ≤39 |
| CV | I-Lyophilized: ≤5.0％,Uketshezi: ＜5.0% |
| I-LoD | Ibhaktheriya eli-1/mL |
| Ukucaciswa | Akukho ukuhlangana kwezakhi zofuzo zomuntu kanye nezinye izifo ezingezona i-Mycobacterium tuberculosis kanye ne-pneumonia |
| Izinsimbi Ezisebenzayo | Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi Sangempela Izinhlelo ze-PCR ze-ABI 7500 ze-Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-LightCycler®480 Zesikhathi Sangempela Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, i-BioRad Uhlelo lwe-PCR lwe-CFX Opus 96 lwesikhathi sangempela |
Isixazululo se-PCR Esiphelele
Inketho 1.
Inketho 2.
