I-Mycobacterium Tuberculosis Rifampicin Ukumelana

Incazelo emfushane:

Le khithi ilungele ukutholwa okufanelekile kokuguqulwa kwe-homozygous ku-507-533 amino acid cocon esifundeni se-RPOB gene evusa i-Mycobacterium Tuberculosis Rifampicin Ukumelana.


Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-RT074A-MyCobacterium Tuberculosis Rifampicin Ukumelana Ukutholwa Kit (Fleorescence PCR)

I-Epidemiology

I-Rifampicin isetshenziswe kabanzi ekwelapheni iziguli zesifo sofuba i-pulmonary kusukela ngasekupheleni kwawo-1970, futhi inomphumela obalulekile. Kube yisinqumo sokuqala sokufushanisa i-chemotherapy yeziguli zesifo sofuba pulmonary. Ukumelana kwe-Rifampicin ikakhulukazi kubangelwa ukuguqulwa kwe-RPOB gene. Yize izidakamizwa ezintsha ze-anti-soturculosis zihlala ziphuma, futhi ukusebenza kahle komtholampilo kweziguli zesifo sofuba pulmonary isifo sofuba sekuqhubekiseleni ukuthuthuka, kusekhona ukuntuleka kwezidakamizwa okulwa nesifo sofuba, futhi into yokusetshenziswa kwezidakamizwa okungenanhlonipho emtholampilo iphakeme kakhulu. Ngokusobala, isifo sofuba se-mycobacterium ezigulini esinezifo ze-pulmonary tuberculosis ngeke sibulawe ngokuphelele ngesikhathi esifanele, okugcina kuholela emaqeleni ahlukene ezidakamizwa emzimbeni wesiguli emzimbeni wesiguli, futhi kwandisa ingozi yokufa kwesiguli. Le khithi ilungele ukuxilongwa okusizayo kokutheleleka kwe-mycobacterium Tuberculosis kanye nokutholwa kohlobo lokumelana nodokotela

I-Epidemiology

 

Igama eliqondisiwe Obikayo I-Quencher
Ukuphendula kwe-BufferA Ukuphendula kwe-BufferB Ukuphendula kwe-BufferC
I-RPOB 507-514 I-RPOB 513-520 IS6110 -Yenza Namunye
I-RPOB 520-527 I-RPOB 527-533 / Cy5 Namunye
/ / Ukulawulwa Kwangaphakathi Hex (VIC) Namunye

 

Amapharamitha Ezobuchwepheshe

Ukubeka

≤-18 ℃ ebumnyameni

Ishalofu-impilo

Izinyanga eziyi-9

Uhlobo lwe-Specimen

Sputum

CV

<5%

Indlu edayisa insimbi

Uhlobo olungamelana ne-Rifampicin - 2x103bacteria / ml

I-homozygous mutant: 2x103bacteria / ml

Ukucacisa

Ithola uhlobo lwe-mycobacterium lwe-mycobacterium kanye nezindawo zokushintshana kwezinye izinhlobo zofuzo zokumelana nezidakamizwa ezifana ne-katg 315g> c \ a, Inhlangano-15c> t, imiphumela yokuhlola ikhombise ukumelana ne-rifampicin, okusho ukuthi akukho ukumelana nokwenza umsebenzi.

Izinsimbi ezisebenzayo:

SLAN-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.)

Ukugeleza komsebenzi


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