I-Mycobacterium Tuberculosis Ukumelana ne-Rifampicin
Igama lomkhiqizo
Ikhithi Yokuthola Ukumelana Nesifo Sofuba i-HWTS-RT074A-Mycobacterium Tuberculosis Rifampicin (i-Fluorescence PCR)
I-Epidemiology
I-Rifampicin ibilokhu isetshenziswa kabanzi ekwelapheni iziguli ezine-TB yamaphaphu kusukela ngasekupheleni kwawo-1970, futhi inethonya elikhulu. Kube yisinqumo sokuqala ukufinyeza ukwelashwa ngamakhemikhali kweziguli ezine-TB yamaphaphu. Ukumelana ne-Rifampicin kubangelwa kakhulu ukuguquka kwezakhi zofuzo ze-rpoB. Nakuba imithi emisha yokulwa nesifo sofuba iphuma njalo, futhi ukusebenza kahle kwezokwelapha kweziguli ezine-TB yamaphaphu nakho kuqhubekile nokuthuthuka, kusenokuntuleka kwemithi yokulwa nesifo sofuba, futhi isimo sokusetshenziswa kwemithi okungenangqondo emtholampilo siphezulu kakhulu. Ngokusobala, isifo sofuba se-Mycobacterium ezigulini ezine-TB yamaphaphu asikwazi ukubulawa ngokuphelele ngesikhathi esifanele, okugcina kuholele emazingeni ahlukene okumelana nemithi emzimbeni wesiguli, kwandise isikhathi sesifo, futhi kwandisa ingozi yokufa kwesiguli. Le khithi ifanele ukuxilongwa okusizayo kokutheleleka kwe-Mycobacterium tuberculosis kanye nokutholakala kwezakhi zofuzo zokumelana ne-rifampicin, okuwusizo ukuqonda ukumelana nemithi kwe-mycobacterium tuberculosis etheleleke yiziguli, kanye nokuhlinzeka ngezindlela ezisizayo zesiqondiso semithi yomtholampilo.
I-Epidemiology
| Igama Eliqondiwe | Intatheli | Isicimi | ||
| I-Reaction BufferA | I-Reaction BufferB | I-Reaction BufferC | ||
| i-rpoB 507-514 | i-rpoB 513-520 | IS6110 | I-FAM | Akukho |
| i-rpoB 520-527 | i-rpoB 527-533 | / | CY5 | Akukho |
| / | / | Ukulawula kwangaphakathi | I-HEX(VIC) | Akukho |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ Ebumnyameni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | Isikhwehlela |
| CV | ＜5％ |
| I-LoD | uhlobo lwasendle olungamelana ne-rifampicin: 2x103amabhaktheriya/mL i-homozygous mutant: 2x103amabhaktheriya/mL |
| Ukucaciswa | Ithola isifo sofuba se-mycobacterium esiwuhlobo lwasendle kanye nezindawo zokuguquka kwezakhi zofuzo ezimelana nemithi njenge-katG 315G>C\A, InhA-15C>T, imiphumela yokuhlolwa ayikhombisi ukumelana ne-rifampicin, okusho ukuthi akukho ukusabela okuxubile. |
| Izinsimbi Ezisebenzayo: | Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) |
Ukugeleza Komsebenzi
