I-Mycobacterium Tuberculosis nuberculosis nucleic acid kanye nokuphikiswa kwe-rifampicin
Igama lomkhiqizo
I-HWTS-RT0744B-MyCobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin Ukumelana Kokutholwa Kokutholwa (Ijika Lokuncibilika)
Isitifiketi
CE
I-Epidemiology
I-Mycobacterium Tuberculosis, kungekudala njenge-tubercle bacillus, i-TB, i-bacterium ye-pathogenic ebangela isifo sofuba. Njengamanje, izidakamizwa ezisetshenziswayo ezisetshenziswayo ezisetshenziswayo ezisetshenziswayo ezisetshenziswayo ezisetshenziswayo zifaka i-isoniazid, izidakamizwa ze-anti-tuberculosol, njll. Izidakamizwa ezintsha ze-anti-tuberculolos zifaka izidakamizwa ezintsha ezithuthukile ziyi-linezolid, e-bedaquiline kanye ne-desamani, njll . Noma kunjalo, ngenxa yokusetshenziswa okungalungile kwe Izidakamizwa ze-anti-tuberculosis kanye nezimpawu zokwakheka kodonga lweseli ye-Mycobacterium Tuberculosis, i-MyCobacterium Tuberculosis ithuthukisa ukumelana nezidakamizwa ezifo ezinogqolozi ezifuywayo, okuletha izinselelo ezinkulu ekuvinjelweni kanye nokwelashwa kwesifo sofuba.
I-Rifampicin isetshenziswe kabanzi ekwelapheni iziguli zesifo sofuba i-pulmonary kusukela ngasekupheleni kwawo-1970, futhi inomphumela obalulekile. Kube yisinqumo sokuqala sokufushanisa i-chemotherapy yeziguli zesifo sofuba pulmonary. Ukumelana kwe-Rifampicin ikakhulukazi kubangelwa ukuguqulwa kwe-RPOB gene. Yize izidakamizwa ezintsha ze-anti-soturculosis zihlala ziphuma, futhi ukusebenza kahle komtholampilo kweziguli zesifo sofuba pulmonary isifo sofuba sekuqhubekiseleni ukuthuthuka, kusekhona ukuntuleka kwezidakamizwa okulwa nesifo sofuba, futhi into yokusetshenziswa kwezidakamizwa okungenanhlonipho emtholampilo iphakeme kakhulu. Ngokusobala, isifo sofuba se-mycobacterium ezigulini esinezifo ze-pulmonary tuberculosis ngeke sibulawe ngokuphelele ngesikhathi esifanele, okugcina kuholela emaqeleni ahlukene ezidakamizwa emzimbeni wesiguli emzimbeni wesiguli, futhi kwandisa ingozi yokufa kwesiguli.
Insungubezi
| Insungubezi | Iziteshi nama-fluorophores | Ukuphendula Buffer a | Ukuphendula Buffer B | I-Reaction Buffer C |
| Isiteshi se-Fam | Intatheli: I-Quencher: Akukho | I-RPOB 507-514 | I-RPOB 513-520 | I-38kd ne-IS6110 |
| Isiteshi seCy5 | Intatheli: CY5, Quencher: Akukho | I-RPOB 520-527 | I-RPOB 527-533 | / |
| Isiteshi se-Hex (VIC) | Intatheli: I-Hex (VIC), i-Quencher: Akukho | Ukulawulwa Kwangaphakathi | Ukulawulwa Kwangaphakathi | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ ebumnyameni |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Sputum |
| CV | ≤5.0% |
| Indlu edayisa insimbi | I-Mycobacterium Tuberculosis 50 Bacteria / ML Uhlobo olungamelana ne-Rifampicin - 2x103bacteria / ml I-homozygous mutant: 2x103bacteria / ml |
| Ukucacisa | Ithola uhlobo lwe-mycobacterium lwe-mycobacterium kanye nezindawo zokushintshana kwezinye izinhlobo zofuzo zokumelana nezidakamizwa ezifana ne-katg 315g> c \ a, Inhlangano-15c> t, imiphumela yokuhlola ikhombise ukumelana ne-rifampicin, okusho ukuthi akukho ukumelana nokwenza umsebenzi. |
| Izinsimbi ezisebenzayo: | SLAN-96P real-time PCRS Systems I-Biorad CFX96 Uhlelo lwe-Real-Time PCR I-Lightcycler480® Real-Time PCRY System |
Ukugeleza komsebenzi
Uma usebenzisa i-Macro & Micro-Test General DNA / RNA KIT (HWTS-3019-50, HWTS-3019-52, HWTS-3019-48, HWS-3019-48, HWS-3019-46, I-HWTS-3019-96, (engasetshenziswa nge-Macro & Micro-Test I-nucleic acid extractor (HWTS-3006C, HWS-3006B)) noma i-Macro & Micro-Test Viral I-DNA / RNA ikholomu (i-HWTS-3022-50) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. yokukhishwa, ukwengeza isampula ye-sputum ukuze ihlolwe ngokulandelana, futhi ungeze I-10μL yokulawulwa kwangaphakathi ngokwehlukana kokulawulwa okuhle, ukulawulwa okungalungile kanye nesampula ye-sputum esetshenzisiwe ukuze ihlolwe, futhi izinyathelo ezilandelayo kufanele zenziwe ngokuqinile ngokuya ngemiyalo yokukhishwa. Ivolumu yesampula ekhishwe i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-100μL.
