I-EasyAmp by Macro & Micro Test - Ithuluzi le-Ispothermal Fluothercence Amplifing Instrateble ehambisane neLamp / RPA / NASBA / HDA / HDA

Ukusebenza okuhle kakhulu nohlelo olubanzi

Easy amp, ngobuchwepheshe be-iI-sothermal nucleic acid amplificationkufakwa nge-highukuzwela futhi-fushaneukuphendulaisikhathi ngaphandle kwezidingo ze inqubo yokushintsha izinga lokushisa. Ngakho-ke, ineokudalaednjenge-kakhuluUbuchwepheshe obuthandekayo bokuhlolwa okusheshayo kwe-nucleic acid.Ngaphandle kwenkimbinkimbi tizinhlelo ze-hermal cycling, idivaysi elula ye-amplification amplificationangagculisa iinqubo yonke yokuphendula.

IMacro & Micro-Test ithuthukiswe ngokuzimelefuthi yethulwei-ealeasAmp,ithuluzi eliphathekayo le-fluorescent ansothermal amplification amplificationNgezicelo ezahlukahlukene.

Okwamanje, TheEasy amp iqinisekisiwe ukubaIyahambisana namasu ambalwa we-isothermal Amplification: I-Loop-Mediated acotheral amplification (isibani), i-polymerase amplification (RPA / Raa / Mira), kanye ne-Helicase), kanye ne-Helicase), kanye neHelicase). Kuzoba ngolunye ithuluzi lokusika elinqenqemeni lokuhlolwa okusheshayo kwe-nucleic acid ngezicelo eziningi eziningi.

· I-loop-mediated asothermal amplification (isibani):I-Amplification at60-65 ° Cnalo lonkeukuphendulaenza ngendlela ethilekuqediwe kungakapheli imizuzu engama-30.Ukubona isithombe 1 ngokukhuliswa kwasoisimiso:

 

Fig 1. Isimisoof Ilambu 

· Recombinase polymerase amplification (RPA / Raa / Mira):I-Amplification at37 ° C-42 ° Cnalo lonkeukuphendulaenza ngendlela ethilekuqedwe ngemizuzu engu-10-30.Ukubona umfanekiso 2 ngobaisilinganiso se-amplification:

Umdwebo 2. Umgomoof I-Recombinase Polymerase Amplification

·I-Nucleic Acid Sequence esekelwe ku-Amplification (NASBA):I-Amplification at42 ° Cnalo lonkeukuphendulaenza ngendlela ethilekuqedwe ngemizuzu engama-40-120.Ukubona isithombe 3 ngobaisilinganiso se-amplification:

Umdwebo 3. Isimisoof I-Nucleic Acid Ukulandelanisa Okusekelwe Ku-Amplification

I-Helicase Ethembele Implifingment (HDA):I-Amplification atI-65 ° Cnalo lonkeukuphendulaenza ngendlela ethilekuqedwe ngemizuzu engama-70-90.Ukubona umfanekiso 4 ngobaisilinganiso se-amplification:

Fig4. Isimisoof IHelicase ancike ukukhula

· I-EasyAmpnika amandlaI-amplification esebenza kahle futhi ezinzile ye-DNA ne-RNA ukutholwa kusetshenziswa amasu amane angenhla. Ifinyelela ukuzwela kokukhulisa kuze kufike kumakhophi angama-200 / ml futhi kuqinisekise ukucaciswa okuphezulu ngaphandle kokusebenzisa imali ngokungemthetho kokukhuliswa kwelitshe.(Ikuvalwe ngezibalo 5 kuya ku-7).

Ukutholwa kwe-DNA

Fig5. Ipulatifomu yeRaa / Mira

Ukutholwa kwe-DNA

Fig6. Isiteji sesibane

 

Ukutholwa kwe-DNA

Fig7. Ipulatifomu ye-nasba

Izinzuzo zomkhiqizo:

-Shesha

· EpiaUbuchwepheshe buyabonaumphumela omuhlekutholwe ku-5 min.

· Ukusebenza okulula ngokukhishwa okukodwaUkuze ukhiphe insimbi.

-Qeqesheka

· 4 amamojula azimele afunwayo, ukuhlolwa kanyekanye okuhlukile okungu-4 okuhlukileUhlobo lokuhlolas.

· Imodyuli ngayinye ekwazi ukuhlaziya amasampula amane ngasikhathi sinye.

· Dual / QuadfI-LUORSCENEnceiziteshi ezikhonangokutholwa okubanzi okuhlosiwe okuningi.

Ukuphathakwa

· Iyahambisana-kaninginingiamapulatifomu anjengesibani anjengesibani, i-RPA, NASBA, HDA.

Decradical for amplication ephezulu yokulwa kokulwa okuphezulu okubhalwe phansi okuphezulu.

· Creatioz-kwaziukuhlangabezana nezidingo ezithile zamakhasimende.

Kuyasebenziseka

· PIdizayini ethandekayo(290×245×1I-28mm, 2kg) ye Ama-Senarios ahlukahlukene Senarios.

· 7-intshi umbala wokuthinta isikriniUkuze uthole imali elula nomfanekiso ocacile wemifanekiso.

Iphrinta eyakhelwe-in thermal-in thermal umphumela wokuphrinta umphumela.

· Isithwebuli esihlanganisiwe sokufundwa kwento ezenzakalelayo.