Ukuhlolwa kwe-EasyAmp nge-Macro kanye ne-Micro—-Ithuluzi Lokukhulisa I-Fluorescence Eliphathekayo Elihambisana ne-LAMP/RPA/NASBA/HDA

Ukusebenza Okuhle Kakhulu Nokusetshenziswa Okubanzi

I-Easy Amp, ngobuchwepheshe be-iukwandiswa kwe-nucleic acid ye-sothermaliboniswa ngokuphezuluukuzwela kanyeokufushaneukusabelaisikhathi esingenazo izidingo ze inqubo yokushintsha izinga lokushisa. Ngakho-ke, ineukuvelaednjengobainingiubuchwepheshe obuthandwayo bokuhlola i-nucleic acid ngokushesha.Ngaphandle kwe-t eyinkimbinkimbiizinhlelo zokujikeleza izitshalo, idivayisi elula futhi ephathekayo yokukhulisa isothermalingakwanelisayonke inqubo yokusabela.

I-Macro & Micro-Test ithuthukiswe ngokuzimelafuthi yethulwai-EasyAmp,ithuluzi lokukhulisa isothermal elikhanyayo eliphathekayokwezinhlelo zokusebenza ezahlukahlukene.

Njengamanje,I-Amp elula kuqinisekiswe ukuthiiyahambisana namasu amaningana okukhulisa i-isothermal: i-Loop-Mediated Isothermal Amplification (LAMP), i-Recombinase Polymerase Amplification (RPA/RAA/MIRA), i-Nucleic Acid Sequence Based Amplification (NASBA), kanye ne-Helicase Dependent Amplification (HDA)Kuzoba elinye ithuluzi eliphambili lokuhlola i-nucleic acid ngokushesha ngezinhlelo zokusebenza ezibanzi kakhulu.

· Ukukhulisa Isothermal Okuphakathi Kwe-Loop (ISIBANI):Ukukhulisa ku-60-65°Cnge-fullukusabelainqubokuqediwe kungakapheli imizuzu engama-30.Ukubona uMfanekiso 1 ukuze uthole ukukhuliswa kwawoisimiso:

 

Umfanekiso 1. Isimisoof ISIBANI 

· Ukukhulisa i-Recombinase Polymerase (RPA/RAA/MIRA):Ukukhulisa ku-37°C-42°Cnge-fullukusabelainqubokuqedwe ngemizuzu eyi-10-30.Ukubona uMfanekiso 2 ngencazelo yawoisimiso sokukhulisa:

Umfanekiso 2. Isimisoof Ukukhulisa i-Recombinase Polymerase

·Ukukhulisa Okusekelwe Ku-Nucleic Acid Sequence (NASBA):Ukukhulisa ku-42°Cnge-fullukusabelainqubokuqedwe ngemizuzu engama-40-120.Ukubona iSithombe 3 sasoisimiso sokukhulisa:

Umfanekiso 3. Isimisoof Ukukhulisa Okusekelwe Ku-Nucleic Acid Sequence

· Ukukhulisa Okuxhomeke Ku-Helicase (HDA):Ukukhulisa ku-65°Cnge-fullukusabelainqubokuqedwe ngemizuzu engama-70-90.Ukubona iSithombe 4 sasoisimiso sokukhulisa:

Isithombe 4. Isimisoof Ukukhulisa Okuxhomeke Ku-Helicase

· I-EasyAmpvumelaUkukhulisa okusebenzayo nokuzinzile kokutholwa kwe-DNA ne-RNA kusetshenziswa amasu amane ashiwo ngenhla. Kufinyelela ukuzwela kokukhulisa kufika kumakhophi angu-200/mL futhi kuqinisekisa ukucaciswa okuphezulu ngaphandle kokusabela okuhlanganisiwe ekukhuliseni okuqondiwe..(Ikuboniswe kuZithombe 5 kuya ku-7).

Ukutholwa kwe-DNA Ukutholwa kwe-RNA

Isithombe 5. Ipulatifomu ye-RAA/MIRA

Ukutholwa kwe-DNA Ukutholwa kwe-RNA

Isithombe 6. Ipulatifomu Yesibani

 

Ukutholwa kwe-DNA Ukutholwa kwe-RNA

Isithombe 7. Ipulatifomu ye-NASBA

Izinzuzo Zomkhiqizo:

Okusheshayo

· I-EPIAubuchwepheshe buyaziumphumela omuhlekutholakale ngemizuzu emi-5.

· Ukusebenza okulula ngokukhipha isinyathelo esisodwayokukhulisa ithuluzi.

Kusebenza kahle

· Amamojula angu-4 azimele okuhlolwa ngasikhathi sinye kwezinhlobo ezi-4 ezahlukene ngesikhathi esifunwayouhlobo lokuhlolas.

· Imodyuli ngayinye ekwazi ukuhlaziya amasampula amane ngesikhathi esisodwa.

· Okubili/Okunefi-luorescenceiziteshiukuthola kabanzi ama-multi-target.

Ukuhambisana

· Iyahambisanaokuningiamapulatifomu asebenzisa i-isothermal njenge-LAMP, i-RPA, i-NASBA, i-HDA.

· Yakhelwe ukukhuliswa kwezinga lokushisa eliphezulu ngemva kokushisa okuphansi kokushintshashintshayo.

· Yenza ngokwezifisouyakwaziukuhlangabezana nezidingo ezithile zamakhasimende.

Kuyasebenziseka

· Pumklamo wetafula ohlelekile(290×245×128mm, 2kg) ye ama-senarios ahlukahlukene esicelo.

· Isikrini sokuthinta esinombala esingu-7 intshiukuze kube lula ukusebenza kanye nomfanekiso ocacile wemiphumela.

· Iphrinta yokushisa eyakhelwe ngaphakathi yokuphrinta imiphumela esheshayo.

· Isithwebuli esihlanganisiwe sokufunda izinto ezitholakala ngokuzenzakalelayo.