I-EasyAmp by Macro & Micro Test—-I-Portable Isothermal Fluorescence Amplification Instrument Ehambisana ne-LAMP/RPA/NASBA/HDA

Ukusebenza Okuhle Kakhulu Nohlelo Olubanzi

I-Easy Amp, ngobuchwepheshe be-ii-sothermal nucleic acid amplificationiboniswa ngokuphezuluukuzwela kanyemfushaneukusabelaisikhathi ngaphandle kwezidingo ze inqubo yokushintsha izinga lokushisa. Ngakho-ke, inevelaednjengeiningiubuchwepheshe obuthandwayo bokuhlolwa okusheshayo kwe-nucleic acid.Ngaphandle kobunzima tamasistimu wamabhayisikili we-hermal, idivayisi elula nephathekayo yokukhulisa i-isothermalanganelisa iyonke inqubo yokusabela.

I-Macro & Micro-Test ithuthukiswe ngokuzimelafuthi yethulwai-EasyAmp,insimbi ephathekayo ye-fluorescent isothermal amplificationkwezinhlelo zokusebenza ezahlukahlukene.

Njengamanje, i-Easy Amp iqinisekisiwe ukuthi injaloIhambisana nezindlela ezimbalwa zokukhulisa i-isothermal: I-Loop-Mediated Isothermal Amplification(LAMP), i-Recombinase Polymerase Amplification (RPA/RAA/MIRA), i-Nucleic Acid Sequence Based Amplification(NASBA), kanye ne-Helicase Dependent Amplification(HDA).Kuzoba elinye ithuluzi eliphambili lokuhlola i-nucleic acid esheshayo enezinhlelo zokusebenza ezibanzi kakhulu.

· I-Loop-Mediated Isothermal Amplification (LAMP):Ukukhulisa ku60-65°Cngayo yonkeukusabelainqubokuqedwe phakathi nemizuzu engama-30.Ukubona uMdwebo 1 wokukhuliswa kwawoisimiso:

 

Umdwebo 1. Isimisoof I-LAMP 

· I-Recombinase Polymerase Amplification (RPA/RAA/MIRA):Ukukhulisa ku37°C-42°Cngayo yonkeukusabelainqubokuqedwe emaminithini angu-10-30.Ukubona uMfanekiso 2 wayoumgomo wokukhulisa umsindo:

Umdwebo 2. Isimisoof I-Recombinase Polymerase Amplification

·I-Nucleic Acid Sequence Based Amplification (NASBA):Ukukhulisa ku42°Cngayo yonkeukusabelainqubokuqedwe ngemizuzu engama-40-120.Ukubona uMfanekiso 3 wayoumgomo wokukhulisa umsindo:

Umfanekiso 3. Isimisoof I-Nucleic Acid Sequence Based Amplification

· I-Helicase Dependent Amplification (HDA):Ukukhulisa ku65°Cngayo yonkeukusabelainqubokuqedwe ngemizuzu engama-70-90.Ukubona uMfanekiso 4 wayoumgomo wokukhulisa umsindo:

Fig4.Isimisoof I-Helicase Dependent Amplification

· I-EasyAmpvumelaukukhulisa okusebenzayo nokuzinzile kokutholwa kwe-DNA kanye ne-RNA kusetshenziswa izindlela ezine ezishiwo ngenhla ze-isothermal.Ifinyelela ukuzwela kokukhulisa kufika kumakhophi angama-200/mL futhi iqinisekisa ukucaciswa okuphezulu ngaphandle kokuphinda kusebenze kabusha ekukhuliseni okuhlosiwe..(Ikuboniswe emifanekisweni 5 kuya ku-7).

Ukutholwa kwe-DNA ye-RNA

Umfanekiso wesi-5.I-RAA/MIRA Platform

Ukutholwa kwe-DNA ye-RNA

Umfanekiso wesi-6.I-LAMP Platform

 

Ukutholwa kwe-DNA ye-RNA

Umfanekiso wesi-7.I-NASBA Platform

Izinzuzo Zomkhiqizo:

Ngokushesha

· I-EPIAubuchwepheshe uyaqaphelaumphumela omuhlekutholwe emizuzwini emi-5.

· Ukusebenza okulula ngesinyathelo esisodwa sokukhiphaokwenzelwe insimbi yomculo.

Isebenza kahle

· Amamojula ama-4 azimele wokuhlolwa okufunekayo, kanyekanye oku-4 ahlukeneuhlobo lokuhlolas.

· Imojula ngayinye ekwazi ukuhlaziya amasampula amane kanye kanye.

· I-Dual/Quadfi-luorescenceiziteshiukuthola okuhloswe ngakho okuningi.

Ukuhambisana

· Iyahambisanaamaningiamapulatifomu e-isothermal afana neLAMP, i-RPA, i-NASBA, i-HDA.

· Idizayinelwe ukulotshwa okuhlanekezela okuhlanekezela kwezinga lokushisa eliphezulu ngemva kokukhulisa izinga lokushisa eliphezulu.

· Yenza ngokwezifisouyakwaziukuhlangabezana nezidingo ezithile zeklayenti.

Kuyasebenziseka

·Pdesign ortable(290×245×128mm, 2kg) ngoba izinhlelo zokusebenza ezahlukene.

· 7-intshi umbala wesikrini esithintwayoukuze isebenze kalula kanye nemiphumela ecacile yomfanekiso.

· Iphrinta eyakhelwe ngaphakathi eshisayo yokuphrinta umphumela osheshayo.

· Isithwebuli esihlanganisiwe sokufundwa kwento etholakala ngokuzenzakalelayo.