—— Ucwaningo Olubambisene olwenziwe yiZhejiang CDC, Macro & Micro-Test, kanye neChina CDC Lushicilelwe ku-Frontiers in Cellular and Infection Microbiology
Ukubuka Konke Kwesifundo
NgoMeyi 2026, i-Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) yashicilela iphepha eliholwa yiZhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), kanye nethimba le-bioinformatics elivela eBeijing Macro & Micro-Test Bio-Tech Co., Ltd. kanye neNational Institute for Communicable Disease Control and Prevention (China CDC) njengababhali ababambisene. Ucwaningo lunesihloko esithi:
"Ukuhlonza kanye nokuhlaziywa kwe-phylogenetic kwezinhlobo eziyisikhombisa ze-Brucella abortus eZhejiang, eShayina."
Lolu cwaningo lumelela ukuhlaziywa kokuqala okuhlelekile, okusekelwe ku-phylogenetic traceability kwe-Brucella abortus (B. abortus) eSifundazweni saseZhejiang, eShayina. Ithimba lihlaziye izinhlobo eziyisikhombisa eziqoqwe kusukela ngo-2015 kuya ku-2025 (izinhlobo ezine ezivela kubantu kanye nezinhlobo ezintathu ezivela ezinkomeni ezivela eJinhua, eQuzhou, naseNingbo). Okutholakele kunikeza ubufakazi be-genomic bemvelaphi kanye nemizila yokudlulisela yalolu "hlobo olusenyakatho oluvelele" esifundeni esingavamile sobhubhane oluseningizimu empumalanga yeShayina.
Isizinda Nokubaluleka Kwaso
I-Brucellosis yisifo esibangelwa yizilwane ezizalwa naso esibangelwa amagciwane ohlobo lwe-Brucella. I-Brucella abortus ihlasela kakhulu izinkomo kodwa ingadala nesifo nakubantu. E-China, i-brucellosis ikhombisa ukuhlukahluka okukhulu kwendawo: ukwenzeka okuphezulu kakhulu kwenzeka ezifundazweni ezisenyakatho (isb., Inner Mongolia, Shanxi, Heilongjiang). Ngokuphambene nalokho, izifundazwe eziseningizimu, okuhlanganisa neZhejiang, bezilokhu zibuswa yi-Brucella melitensis, kanti ambalwa kakhulu amacala abikiwe e-B. abortus. Lokhu kungafani kwesifunda kwenza ukuhlukaniswa kwezakhi zofuzo kanye nokulandelela umthombo we-B. abortus eZhejiang kube yinto ebalulekile empilweni yomphakathi.
Izindlela kanye Nokutholakele Okubalulekile
Ithimba locwaningo lamukele isu elihlanganisa i-molecular biology kanye ne-bioinformatics:
1.Ukuhlonza i-pathogen kanye nokuthayipha okuyisisekelo
I-BCSP-31 gene PCR kanye ne-AMOS-PCR ziqinisekisile ukuthi zonke izinhlobo eziyisikhombisa ze-isolates zaziyi-B. abortus.
Ukuthayipha kwe-Multilocus sequence (MLST) okusekelwe ku-housekeeping genes eziyisishiyagalolunye kwembule ukuthi zonke izinhlobo ze-isolates zazingezohlobo lwe-sequence ST2, okubonisa ukulingana okuphezulu kwezakhi zofuzo phakathi kwezinhlobo ze-B. abortus ezijikelezayo eZhejiang.
2.Ukuhlukaniswa kwe-genome ephelele
Ukuhlolwa kwe-genome ephelele kwenziwe epulatifomu ye-Illumina NovaSeq. Ukuhlaziywa kwe-nucleotide identity (ANI) okujwayelekile kubonise ukuthi ama-isolates e-Zhejiang abelana ngokufana okufika ku-99.99% nohlobo lwe-reference B. abortus 544.
Ukuhlaziywa kwe-Pan-genome kwembule inani labantu eligcinwe kahle kakhulu: kwatholakala izakhi zofuzo ezingu-3,084 eziyinhloko, kanye nezakhi zofuzo ezingu-10 kuphela zegobolondo, futhi akutholwanga zakhi zofuzo ezithambile noma zamafu eziyinhloko.
3.Amaphrofayili ezakhi zofuzo zokumelana negciwane kanye nokulwa namagciwane
Kubikezelwe izici ezingu-68 ezihlobene nobungozi, ezihlanganisa izindlela zakudala ezifana ne-LPS biosynthesis, uhlelo lokukhipha i-T4SS, kanye nohlelo lokulawula olunezingxenye ezimbili lwe-BvrR-BvrS. Okuphawulekayo ukuthi zonke izinhlobo zofuzo zazingenazo izakhi zofuzo ze-adhesin i-bmaA kanye ne-btaF. Ukuhlaziywa kwezakhi zofuzo zokumelana kuthole kuphela i-mprF gene kusizindalwazi se-CARD, kungekho ezinye izici zokumelana ezitholiwe.
4. Ukwakhiwa kabusha kwe-phylogenetic kanye nokulandelela ukudluliswa
Ukuhlaziywa kwe-Core-genome single-nucleotide polymorphism (cgSNP) kubeke ama-isolate aseZhejiang endaweni ethile esihlahleni se-phylogenetic somhlaba wonke. Imiphumela ibonise ukuthi izinhlobo zeZhejiang zakha iqembu le-monophyletic kanye nezinhlobo ezivela eRussia, eMongolia, kanye nezifundazwe eziningana zasenyakatho yeShayina (iNingxia, iHeilongjiang, i-Inner Mongolia, iHebei, iGansu, neBeijing). Leli qembu liphinde lihlukaniswe laba ama-sub clade amathathu ahlukene (i-Clade 1-3), okuphakamisa izehlakalo eziningi zokwethula ezizimele.
Iziphetho kanye Nemiphumela
Lolu cwaningo luhlinzeka ngedatha yokuqala ye-genomic enembile kakhulu ye-B. abortus eSifundazweni saseZhejiang futhi luveza iziphetho eziningana ezibalulekile:
- I-Clear isizinda sofuzo– Izinhlobo ze-B. abortus ezisakazeka eZhejiang zingezakwa-ST2, zilondolozwe kakhulu ngokwezakhi zofuzo, futhi zimelela uhlu olujwayelekile lwe-brucellosis yezinkomo.
2. I-Eviumkhawulo wokudluliselwa kwesifunda esinqamulayo– Ukuhlaziywa kwe-phylogenetic akusekeli ukuba khona kozalo oluzimele oluhlala njalo eZhejiang. Esikhundleni salokho, idatha isikisela ngokuqinile ukuthi lezi zinhlobo zivela enyakatho yeShayina futhi zingabelana ngesizinda esifanayo sokuziphendukela kwemvelo nezinhlobo ezivela eRussia naseMongolia. Ukuba khona kwezinhlobo ezintathu ezincane kusho izehlakalo eziningi zokwethulwa ezihlukene.
3. Imiphumela yezempilo yomphakathi– Okutholakele kugcizelela ukubaluleka kokubhekwa kwe-genomic kwe-brucellosis ngisho nasezindaweni ezingezona ezivamile njengeZhejiang. Nakuba inani lamacala amanje liphansi, amathuluzi okuxazulula izinkinga aphezulu njenge-cgSNP angalandelela ngempumelelo umthombo wokuqubuka kwaleli gciwane futhi anikeze ubufakazi besayensi bokuphazamisa uchungechunge lokudlulisela oluhlobene nokuthuthwa kwemfuyo phakathi kwezifundazwe.
Lo msebenzi awugcini nje ngokugcwalisa igebe locwaningo eSifundazweni saseZhejiang kodwa futhi uhlinzeka ngemininingwane emisha eyisisekelo yokuqapha izifo kanye nokuhlola ubungozi be-brucellosis esifundeni saseYangtze River Delta.
Ulwazi Lwephepha:
UYang, Y., uShi, X., uChen, J., uWang, L., uWu, Z., uYao, W., … noWu, B. (2026). Ukuhlonza kanye nokuhlaziywa kwe-phylogenetic kwezinhlobo eziyisikhombisa ze-Brucella abortus eZhejiang, eShayina. I-Frontiers in Cellular and Infection Microbiology, 16, 1758965.
Isikhathi sokuthunyelwe: Juni-10-2026