Isikhali Esisha Sokuxilongwa Kwesifo Sofuba Nokutholwa Kokumelana Nezidakamizwa: Ukulandelanisa Okuhlosiwe Kwesizukulwane Esisha (tNGS) Kuhlanganiswe Nokufunda Ngomshini Kwesifo Sofuba I-hypersensitivity Diagnosis
Umbiko wezincwadi: I-CCa: imodeli yokuxilonga esekelwe ku-tNGS nokufunda komshini, elungele abantu abanesifo sofuba esinegciwane elincane kanye ne-meningitis ye-tuberculous.
Isihloko se-Thesis: Ukulandelana kwesizukulwane esilandelayo esihloselwe i-Tuberculous nokufunda komshini: isu lokuxilonga elizwela kakhulu lama-paucific pulmonary tubulars kanye ne-tubular meningitis.
Isikhathi: 《Clinica Chimica Acta》
IF:6.5
Idethi yokushicilelwa: Januwari 2024
Ihlanganiswe ne-University of Chinese Academy of Sciences kanye ne-Beijing Chest Hospital yase-Capital Medical University, i-Macro & Micro-Test isungule imodeli yokuxilongwa kwesifo sofuba esekelwe esizukulwaneni esisha sobuchwepheshe bokulandelana okuhlosiwe (tNGS) kanye nendlela yokufunda ngomshini, ehlinzeka ngokuphezulu kakhulu. ukuthola ukuzwela kwesifo sofuba esinamagciwane ambalwa kanye nemeningitis yofuba, kunikeze indlela entsha yokuxilongwa kwe-hypersensitivity yokuxilongwa emtholampilo kwezinhlobo ezimbili zesifo sofuba, futhi yasiza ekuxilongeni okunembile, ukutholwa kokumelana nezidakamizwa kanye nokwelashwa kwesifo sofuba.Ngasikhathi sinye, kutholakala ukuthi i-plasma cfDNA yesiguli ingasetshenziswa njengohlobo lwesampula olufanelekile lwesampula lomtholampilo ekuxilongweni kwe-TBM.
Kulolu cwaningo, amasampula e-plasma angama-227 kanye namasampula oketshezi lwe-cerebrospinal asetshenziselwa ukusungula amaqoqo amabili emitholampilo, lapho amasampula eqembu lokuxilonga elabhorethri asetshenziselwa ukusungula imodeli yokufunda ngomshini yokuxilongwa kwesifo sofuba, kanye namasampula eqembu lokuxilonga emtholampilo asetshenziselwa ukuqinisekisa imodeli yokuxilonga.Wonke amasampuli aqale akhonjwa iphuli yophenyo eqondiswe ngokukhethekile ye-Mycobacterium tuberculosis.Bese, ngokususelwe kudatha yokulandelana kwe-TB-tNGS, imodeli yesihlahla sesinqumo isetshenziselwa ukwenza ukuqinisekiswa okuphindwe izikhathi ezi-5 kumasethi okuqeqesha nokuqinisekisa omugqa wokuxilonga elabhorethri, kanye nomkhawulo wokuxilonga wamasampula e-plasma kanye namasampula oketshezi lwe-cerebrospinal ayatholakala.Umkhawulo otholiwe ulethwa kumasethi amabili okuhlola omugqa wokuxilongwa komtholampilo ukuze utholwe, futhi ukusebenza kokuxilonga komfundi kuhlolwa ijika le-ROC.Ekugcineni, kwatholakala imodeli yokuxilongwa kwesifo sofuba.
Umdwebo ongu-1 wohlelo lomklamo wocwaningo
Imiphumela: Ngokwemikhawulo ethile yesampula ye-CSF DNA (AUC = 0.974) kanye nesampula ye-plasma cfDNA (AUC = 0.908) enqunywe kulolu cwaningo, phakathi kwamasampuli angu-227, ukuzwela kwesampula ye-CSF kwakungu-97.01%, ukucaciswa kwakungu-95.65%, futhi ukuzwela kanye nokucaciswa kwesampula ye-plasma bekungama-82.61% kanye nama-86.36%.Ekuhlaziyweni kwamasampula abhanqiwe angama-44 e-plasma cfDNA kanye ne-cerebrospinal fluid DNA evela ezigulini ze-TBM, isu lokuxilonga lalolu cwaningo linokuqina okuphezulu okungu-90.91% (40/44) kuplasma cfDNA kanye ne-cerebrospinal fluid DNA, futhi ukuzwela kungu-95.45% (42/44).Ezinganeni ezinesifo sofuba samaphaphu, isu lokuxilonga lalolu cwaningo lizwela kakhulu kumasampula e-plasma kunemiphumela yokutholwa kwe-Xpert yamasampula ejusi yesisu ezigulini ezifanayo (28.57% VS 15.38%).
Umdwebo 2 Ukuhlaziywa kokusebenza kwemodeli yokuxilongwa kwesifo sofuba kumasampula abantu
