Izinhlobo Eziyisishiyagalolunye Zamagciwane Okuphefumula
Igama lomkhiqizo
I-HWTS-RT185A-Nine Izinhlobo Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Ukutheleleka ngomgudu wokuphefumula kuwuhlobo oluvame kakhulu lwesifo somuntu, esingenzeka kunoma ibuphi ubulili, ubudala kanye nesifunda, futhi singenye yezimbangela ezibaluleke kakhulu zokugula nokufa emhlabeni.[1]. Amagciwane ajwayelekile okuphefumula ahlanganisa igciwane lomkhuhlane A (IFV A), igciwane lomkhuhlane B (IFV B), inoveli coronavirus (SARS-CoV-2), i-respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) kanye ne-mycoplasma pneumoniae, njll.[2,3]. Izimpawu zomtholampilo nezimpawu ezibangelwa ukutheleleka ngomgudu wokuphefumula ziyefana, kodwa ukutheleleka okubangelwa amagciwane ahlukahlukene kunezindlela ezihlukene zokwelapha, imiphumela yokwelapha kanye nenkambo yezifo.[4,5]. Njengamanje, izindlela eziyinhloko zokutholwa kwamagciwane e-laboratory zifaka: ukuhlukaniswa kwegciwane, ukutholwa kwe-antigen kanye nokutholwa kwe-nucleic acid. Le kit ithola futhi ihlonze i-viral nucleic acid ethize kubantu abanezimpawu nezimpawu zokutheleleka kokuphefumula, ngokuhambisana neminye imiphumela yomtholampilo neyelabhorethri ukusiza ekuxilongeni ukutheleleka ngegciwane lokuphefumula.
Isiteshi
| FAM | I-MP nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | 2-8℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-oropharyngeal swab; I-Nasopharyngeal swab |
| Ct | I-COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 amakhophi/mL |
| Ukucaciswa | I-Cross reactivity: Akukho ukuphinda kusebenze phakathi kwekhithi ne-Boca virus, i-Cytomegalovirus, i-Epstein-Barr virus, i-Herpes simplex virus, i-varicella zoster Virus, i-Mumps virus, i-Enterovirus, igciwane lesimungumungwane, i-human coronavirus, i-SARS Coronavirus, i-MERS Coronavirus, i-Rotavirus, i-Norovirus, i-Chlamydia pneumoniae, i-pneumoniasiptococcus Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Moralicoccus, Streptococcus calictococcus, salictococcus I-Corynebacterium, i-DNA ye-genomic yomuntu. Ukuhlolwa kokuphazanyiswa: Khetha i-mucin (60mg/mL), igazi lomuntu (50%), i-benephrin (2mg/mL), i-hydroxymethazoline (2mg/mL) 2mg/mL), i-sodium chloride eno-5% wokulondolozwa (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone/mL0mg/mL0), i-dexamethasone/mL0mg/mL triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), peramivir (1mg/mL), peramivir (1mg/mL) (0.6mg/mL), oseltamivir (60ng/mL), i-ribavirin (10mg/L), Imiphumela yabonisa ukuthi izinto eziphazamisayo ekugxilweni okungenhla azinakho ukusabela okuphazamisayo ekutholweni kwekhithi. |
| Izinsimbi Ezisebenzayo | Isebenza ekuthayipheni i-reagent yokuthola i-I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 I-Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Radzhou Time PC. Isistimu, i-BioRad CFX Opus 96 Real-Time PCR System. Isebenza kuhlobo lwe-II esebenzayo yokuthola: I-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
| Isitoreji | 2-8℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-oropharyngeal swab; I-Nasopharyngeal swab |
| Ct | I-COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 amakhophi/mL |
| Ukucaciswa | I-Cross reactivity: Akukho ukuphinda kusebenze phakathi kwekhithi ne-Boca virus, i-Cytomegalovirus, i-Epstein-Barr virus, i-Herpes simplex virus, i-varicella zoster Virus, i-Mumps virus, i-Enterovirus, igciwane lesimungumungwane, i-human coronavirus, i-SARS Coronavirus, i-MERS Coronavirus, i-Rotavirus, i-Norovirus, i-Chlamydia pneumoniae, i-pneumoniasiptococcus Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Moralicoccus, Streptococcus calictococcus, salictococcus I-Corynebacterium, i-DNA ye-genomic yomuntu. Ukuhlolwa kokuphazanyiswa: Khetha i-mucin (60mg/mL), igazi lomuntu (50%), i-benephrin (2mg/mL), i-hydroxymethazoline (2mg/mL) 2mg/mL), i-sodium chloride eno-5% wokulondolozwa (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone/mL0mg/mL0), i-dexamethasone/mL0mg/mL triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), peramivir (1mg/mL), peramivir (1mg/mL) (0.6mg/mL), oseltamivir (60ng/mL), i-ribavirin (10mg/L), Imiphumela yabonisa ukuthi izinto eziphazamisayo ekugxilweni okungenhla azinakho ukusabela okuphazamisayo ekutholweni kwekhithi. |
| Izinsimbi Ezisebenzayo | Isebenza ekuthayipheni i-reagent yokuthola i-I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 I-Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Radzhou Time PC. Isistimu, i-BioRad CFX Opus 96 Real-Time PCR System. Isebenza kuhlobo lwe-II esebenzayo yokuthola: I-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-17u-80 esetshenziswa)TM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ivolumu yesampula ekhishiwe ingu-200μL futhi ivolumu ye-lution enconyiwe ingu-150μL.
