Izinhlobo Eziyisishiyagalolunye Zamagciwane Okuphefumula

Incazelo emfushane:

Le khithi isetshenziselwa ukutholwa kwe-in vitro qualitative virus ye-influenza A virus (IFV A), i-influenza B virus (IFVB), inoveli coronavirus (SARS-CoV-2), i-respiratory syncytial virus (RSV), i-adenovirus (Adv), i-human metapneumovirus (hMPV), i-rhinovirus (RhV), igciwane le-myPIV/III (myPIV) i-pneumoniae (MP) ama-nucleic acid ku-human oropharyngeal swab kanye namasampula e-nasopharyngeal swab.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-RT185A-Izinhlobo Eziyisishiyagalolunye Zamagciwane Okuphefumula I-Nucleic Acid Detection Kit (i-Fluorescence PCR)

I-Epidemiology

Ukutheleleka kwezifo zokuphefumula uhlobo oluvame kakhulu lwesifo kubantu, olungavela kunoma yiluphi ubulili, ubudala kanye nesifunda, futhi lungenye yezimbangela ezibaluleke kakhulu zokugula nokufa emhlabeni.^[1]. Amagciwane ajwayelekile okuphefumula ahlanganisa igciwane lomkhuhlane A (IFV A), igciwane lomkhuhlane B (IFV B), inoveli coronavirus (SARS-CoV-2), i-respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) kanye ne-mycoplasma pneumoniae, njll.^[2,3]Izimpawu zomtholampilo nezimpawu ezibangelwa ukutheleleka kwendlela yokuphefumula ziyafana, kodwa ukutheleleka okubangelwa amagciwane ahlukene kunezindlela ezahlukene zokwelapha, imiphumela yokwelapha kanye nendlela isifo esiqhubeka ngayo.^[4,5]Njengamanje, izindlela eziyinhloko zokuthola izifo zokuphefumula elabhorethri zifaka: ukuhlukaniswa kwegciwane, ukutholwa kwe-antigen kanye nokutholwa kwe-nucleic acid. Le khithi ithola futhi ihlonze ama-virtual nucleic acid athile kubantu abanezimpawu zokutheleleka kokuphefumula, kanye neminye imiphumela yezokwelapha neyelabhorethri ukusiza ekuxilongweni kokutheleleka kwegciwane lokuphefumula.

Isiteshi

I-FAM	i-asidi ye-nucleic ye-MP
I-ROX	Ukulawula Kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	2-8℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-Oropharyngeal swab; I-Nasopharyngeal swab
Ct	I-COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35
CV	<5.0%
I-LoD	Amakhophi angu-200/mL
Ukucaciswa	Ukusabela okuxubile: Akukho ukusabela okuxubile phakathi kwekhithi negciwane leBoca, i-Cytomegalovirus, igciwane le-Epstein-Barr, igciwane le-Herpes simplex, igciwane le-varicella zoster, igciwane le-Mumps, igciwane le-Enterovirus, igciwane le-measles, igciwane le-human coronavirus, i-SARS Coronavirus, i-MERS Coronavirus, i-Rotavirus, i-Norovirus, i-Chlamydia pneumoniae, i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Streptococcus pyogenes, i-Legionella, i-Pneumospora, i-Haemophilus influenzae, i-Bacillus pertussis, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-gonococcus, i-Candida albicans, i-Candida glabra, i-Aspergillus fumigatus, i-Cryptococcus neoformans, i-Streptococcus salivarius, i-Moraxella catarrh, i-Lactobacillus, i-Corynebacterium, i-human genomic DNA. Ukuhlolwa kokuphazamiseka: Khetha i-mucin (60mg/mL), igazi lomuntu (50%), i-benephrin (2mg/mL), i-hydroxymethazoline (2mg/mL) 2mg/mL), i-sodium chloride ene-5% yokuvikela (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-fluniacetone (20μg/mL), i-triamcinolone (2mg/mL), i-budesonide (1mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-benzocaine (10%), i-menthol (10%), i-zanamivir (20mg/mL), i-peramivir (1mg/mL), i-mupirocin (20mg/mL), i-tobramycin (0.6mg/mL), i-oseltamivir (60ng/mL), i-ribavirin (10mg/L), Imiphumela ikhombisile ukuthi izinto eziphazamisayo ekugxilweni okungenhla azizange ziphazamise ukutholwa kwekhithi.
Izinsimbi Ezisebenzayo	Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-Applied Biosystems 7500 Real-Time PCR, i-QuantStudio^®Izinhlelo ezi-5 ze-PCR Zesikhathi Sangempela, Izinhlelo ze-SLAN-96P Zesikhathi Sangempela ze-PCR (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Zesikhathi Sangempela ze-PCR (i-FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (i-Suzhou Molarray Co., Ltd.), Uhlelo lwe-PCR lwe-BioRad CFX96 Real-Time, Uhlelo lwe-PCR lwe-BioRad CFX Opus 96 Real-Time. Kusebenza ku-reagent yokuthola uhlobo lwesibili: i-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Isitoreji	2-8℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	I-Oropharyngeal swab; I-Nasopharyngeal swab
Ct	I-COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35
CV	<5.0%
I-LoD	Amakhophi angu-200/mL
Ukucaciswa	Ukusabela okuxubile: Akukho ukusabela okuxubile phakathi kwekhithi negciwane leBoca, i-Cytomegalovirus, igciwane le-Epstein-Barr, igciwane le-Herpes simplex, igciwane le-varicella zoster, igciwane le-Mumps, igciwane le-Enterovirus, igciwane le-measles, igciwane le-human coronavirus, i-SARS Coronavirus, i-MERS Coronavirus, i-Rotavirus, i-Norovirus, i-Chlamydia pneumoniae, i-Streptococcus pneumoniae, i-Klebsiella pneumoniae, i-Streptococcus pyogenes, i-Legionella, i-Pneumospora, i-Haemophilus influenzae, i-Bacillus pertussis, i-Staphylococcus aureus, i-Mycobacterium tuberculosis, i-gonococcus, i-Candida albicans, i-Candida glabra, i-Aspergillus fumigatus, i-Cryptococcus neoformans, i-Streptococcus salivarius, i-Moraxella catarrh, i-Lactobacillus, i-Corynebacterium, i-human genomic DNA. Ukuhlolwa kokuphazamiseka: Khetha i-mucin (60mg/mL), igazi lomuntu (50%), i-benephrin (2mg/mL), i-hydroxymethazoline (2mg/mL) 2mg/mL), i-sodium chloride ene-5% yokuvikela (20mg/mL), i-beclomethasone (20mg/mL), i-dexamethasone (20mg/mL), i-fluniacetone (20μg/mL), i-triamcinolone (2mg/mL), i-budesonide (1mg/mL), i-mometasone (2mg/mL), i-fluticasone (2mg/mL), i-histamine hydrochloride (5mg/mL), i-benzocaine (10%), i-menthol (10%), i-zanamivir (20mg/mL), i-peramivir (1mg/mL), i-mupirocin (20mg/mL), i-tobramycin (0.6mg/mL), i-oseltamivir (60ng/mL), i-ribavirin (10mg/L), Imiphumela ikhombisile ukuthi izinto eziphazamisayo ekugxilweni okungenhla azizange ziphazamise ukutholwa kwekhithi.
Izinsimbi Ezisebenzayo	Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-Applied Biosystems 7500 Real-Time PCR, i-QuantStudio^®Izinhlelo ezi-5 ze-PCR Zesikhathi Sangempela, Izinhlelo ze-SLAN-96P Zesikhathi Sangempela ze-PCR (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Zesikhathi Sangempela ze-PCR (i-FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (i-Suzhou Molarray Co., Ltd.), Uhlelo lwe-PCR lwe-BioRad CFX96 Real-Time, Uhlelo lwe-PCR lwe-BioRad CFX Opus 96 Real-Time. Kusebenza ku-reagent yokuthola uhlobo lwesibili: i-EudemonTM AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Ukugeleza Komsebenzi

I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-Eudemon^TMI-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.

Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.

Okwedlule: Igciwane leMonkeypox kanye neTyping Nucleic Acid

Olandelayo: Izinhlobo Eziyisishiyagalombili Zamagciwane Okuphefumula

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