Ikhithi Yokuthola I-Neisseria Gonorrhoeae Nucleic Acid

Ikhithi Yokuthola I-Nucleic Acid ye-Neisseria Gonorrhoeae Isithombe Esifakiwe

Incazelo emfushane:

Le khithi yenzelwe ukutholwa kwe-Neisseria Gonorrhoeae(NG) nucleic acid emchameni wesilisa, i-urethral swab yesilisa, amasampula e-cervical swab yesifazane.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama Lomkhiqizo

Ikhithi Yokuthola I-Neisseria Gonorrhoeae Nucleic Acid

I-Epidemiology

I-Gonorrhea yisifo esivamile esidluliselwa ngocansi esibangelwa ukutheleleka nge-Neisseria gonorrhoeae (NG), esibonakala kakhulu njengokuvuvukala kwe-purulent kwe-mucous membranes yesistimu ye-genitourinary. I-NG ingahlukaniswa ngezinhlobo eziningana ze-ST. I-NG ingangena ohlelweni lwe-genitourinary futhi izale, ibangele i-urethritis kwabesilisa, i-urethritis kanye ne-cervicitis kwabesifazane. Uma ingelashwa kahle, ingasakazekela ohlelweni lokuzala. Ingane ingatheleleka ngomsele wokuzalwa okuholela ku-gonorrhea yezingane ezisanda kuzalwa i-conjunctivitis ebukhali. Abantu abanawo amasosha omzimba emvelo ku-NG futhi basengozini ye-NG. Abantu banamandla omzimba abuthakathaka ngemva kokutheleleka angenakukuvimbela ukutheleleka kabusha.

Isiteshi

I-FAM	Inhloso ye-NG
I-VIC(HEX)	Ukulawula Kwangaphakathi

Ukusetha Izimo Zokukhulisa ze-PCR

Isitoreji	Uketshezi: ≤-18℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Ukukhishelwa komchamo kwabesilisa, Umchamo wabesilisa, Ukukhishelwa komchamo kwabesifazane
Ct	≤38
CV	≤5.0%
I-LoD	Amakhophi angu-50/ukusabela
Ukucaciswa	Akukho ukuhlangana kabusha namanye amagciwane e-STD, njenge-Treponema pallidum, i-Chlamydia trachomatis, i-Ureaplasma urealyticum, i-Mycoplasma hominis, i-Mycoplasma genitalium nokunye.
Izinsimbi Ezisebenzayo	Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe. Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Uhlelo lwe-PCR olusetshenziswayo lwe-Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio® 5 Zesikhathi Sangempela Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR

Ukuhamba komsebenzi

Ikhithi Yokuthola I-Neisseria Gonorrhoeae Nucleic Acid

Kudingeka i-reagent kodwa ayinikezwanga

I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8), I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., isixazululo sokulondolozwa kwe-saline evamile, umchamo.

Izinto ezisetshenziswayo ziyadingeka kodwa azinikezwanga

Amathiphu angenayo i-RNase/DNase, amagilavu alahlwayo, amashubhu angenayo i-RNase/DNase, i-centrifuge, isitendi sikamazibuthe, imichilo yamashubhu angu-8 ye-PCR.

Okwedlule: Ukutholwa kwe-Nucleic Acid Group B Streptococcus (EPIA)

Olandelayo: Ikhithi Yokuthola I-Herpes Simplex Virus Type 2 Nucleic Acid (i-Fluorescence PCR)

Bhala umlayezo wakho lapha bese uwuthumela kithi