I-DNA ye-Mycobacterium Tuberculosis

Isithombe Esifakiwe se-DNA ye-Mycobacterium Tuberculosis

Incazelo emfushane:

Le khithi isetshenziselwa ukuthola iziguli ezinezimpawu/izimpawu ezihlobene nesifo sofuba ngaphakathi kwesibeletho noma iqinisekiswe ngokuhlolwa kwe-X-ray kokutheleleka kwesifo sofuba se-mycobacterium kanye nezibonelo ze-sputum zeziguli ezidinga ukuxilongwa noma ukuxilongwa okuhlukile kokutheleleka kwesifo sofuba se-mycobacterium.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Ikhithi Yokuthola I-HWTS-RT102-Nucleic Acid esekelwe ku-Enzymatic Probe Isothermal Amplification (EPIA) yesifo sofuba se-Mycobacterium

Isitifiketi

I-Epidemiology

I-Mycobacterium tubercle tubercle (i-Tubercle bacillus, i-TB) uhlobo lwebhaktheriya ye-aerobic ebophezelayo ene-acid-fast staining enhle. Kukhona i-pili ku-TB kodwa ayikho i-flagellum. Nakuba i-TB inama-microcapsules kodwa ayakhi ama-spores. Udonga lweseli lwe-TB alunawo i-teichoic acid yamabhaktheriya ane-gram noma i-lipopolysaccharide yamabhaktheriya ane-gram. I-Mycobacterium tuberculosis ebangela izifo kubantu ngokuvamile ihlukaniswe ngohlobo lwabantu, uhlobo lwezinkomo, kanye nohlobo lwase-Afrika. Ubungozi be-TB bungahlotshaniswa nokuvuvukala okubangelwa ukwanda kwamabhaktheriya kumaseli ezicubu, ubuthi bezingxenye zamabhaktheriya kanye nama-metabolites, kanye nomonakalo wokuzivikela komzimba ezingxenyeni zamabhaktheriya. Izinto ezibangela izifo zihlobene nama-capsules, amafutha kanye namaprotheni. I-Mycobacterium tuberculosis ingahlasela inani labantu abasengozini ngokusebenzisa indlela yokuphefumula, indlela yokugaya ukudla noma umonakalo wesikhumba, okubangela isifo sofuba ezinhlobonhlobo zezicubu nezitho zomzimba, lapho isifo sofuba esibangelwa indlela yokuphefumula sisona esivame kakhulu. Sivela kakhulu ezinganeni, sinezimpawu ezifana nomkhuhlane ophansi, ukujuluka ebusuku, kanye nenani elincane le-hemoptysis. Ukutheleleka kwesibili kubonakala kakhulu njengomkhuhlane ophansi, ukujuluka ebusuku, i-hemoptysis nezinye izimpawu; ukuqala okungapheli, ukuhlaselwa okumbalwa okukhulu. Isifo sofuba singenye yezimbangela eziyishumi eziphambili zokufa emhlabeni. Ngo-2018, abantu ababalelwa ezigidini eziyi-10 emhlabeni batheleleka ngesifo sofuba i-Mycobacterium, abantu ababalelwa ezigidini eziyi-1.6 bafa. I-China iyizwe elinomthwalo omkhulu wesifo sofuba, futhi izinga lokutheleleka kwaso lisezingeni lesibili emhlabeni.

Isiteshi

I-FAM	Isifo sofuba se-Mycobacterium
CY5	Ukulawula kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	Uketshezi: ≤-18℃;
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Isikhwehlela
Tt	≤28
CV	≤10％
I-LoD	Uketshezi: Amakhophi angu-1000/mL,
Ukucaciswa	Akukho ukuhlangana kwe-mycobacteria namanye ama-mycobacteria ku-non-Mycobacterium tuberculosis complex (isb. Mycobacterium kansas, Mycobacter surga, Mycobacterium marinum, njll.) kanye nezinye izifo (isb. Streptococcus pneumoniae, Haemophilus influenzae, Escherichia coli, njll.).
Izinsimbi Ezisebenzayo (Uketshezi)	Uhlelo Lokuthola Isothermal lwe-Easy Amp Real-time Fluorescence (HWTS1600),Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time,Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.)
Izinsimbi Ezisebenzayo (Ezifakwe I-Lyophilized)	Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (Shanghai Hongshi Medical Technology Co., Ltd.) I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola Ukushisa Okungaguquguquki Kwe-Fluorescence Yesikhathi Sangempela I-Easy Amp HWTS1600