Izinhlobo eziyisithupha zamagciwane okuphefumula

Incazelo emfushane:

Le khithi ingasetshenziswa ukuthola ngokufanelekile i-nucleic acid ye-SARS-CoV-2, igciwane lomkhuhlane A, igciwane lomkhuhlane B, i-adenovirus, i-mycoplasma pneumoniae kanye negciwane lokuphefumula le-sycytial in vitro.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-OT058A/B/C/Z-Real time fluorescent RT-PCR kit yokuthola izinhlobo eziyisithupha zamagciwane okuphefumula

Isitifiketi

I-Epidemiology

I-Corona Virus Disease 2019, ebizwa ngokuthi “i-COVID-19”, isho inyumoniya ebangelwa ukutheleleka kwe-SARS-CoV-2.I-SARS-CoV-2 iyi-coronavirus yohlobo lwe-β.I-COVID-19 yisifo esithathelwanayo esinamandla sokuphefumula, futhi inani labantu ngokuvamile liyathinteka.Njengamanje, umthombo wokutheleleka ikakhulukazi iziguli ezingenwe yi-SARS-CoV-2, futhi abantu abangenwe yi-asymptomatic bangase babe umthombo wokutheleleka.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezingu-1-14, ikakhulukazi izinsuku ezingu-3-7.Umkhuhlane, ukukhwehlela okomile nokukhathala yizimpawu eziyinhloko.Iziguli ezimbalwa zazinokuminyana emakhaleni, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo.

Umkhuhlane, owaziwa ngokuthi "umkhuhlane", isifo esithathelwanayo sokuphefumula esibangelwa igciwane lomkhuhlane.Iyathelelana kakhulu.Isakazwa kakhulu ngokukhwehlela kanye nokuthimula.Ivamise ukuqubuka entwasahlobo nasebusika.Amagciwane oMkhuhlane ahlukene abe umkhuhlane A, IFV A, umkhuhlane B, IFV B, kanye noMkhuhlane C, IFV C izinhlobo ezintathu, zonke zingamagciwane anamathelayo, abangela isifo somuntu ikakhulukazi kumagciwane omkhuhlane A no-B, iyi-stranded eyodwa, i-RNA virus ehlukene.Igciwane loMkhuhlane A liwukutheleleka kokuphefumula okunamandla, okuhlanganisa i-H1N1, i-H3N2 nezinye izinhlobo ezincane, ezivame ukuguquka nokuqubuka emhlabeni jikelele.I-"Shift" ibhekisela ekuguqulweni kwegciwane lomkhuhlane A, okuholela ekuveleni kwegciwane elisha "i-subtype".Amagciwane oMkhuhlane B ahlukaniswe izigaba ezimbili, i-Yamagata ne-Victoria.Igciwane lomkhuhlane B line-antigenic drift kuphela, futhi ligwema ukubhekwa nokuqedwa kwamasosha omzimba ngokuguqulwa kwawo.Kodwa-ke, ijubane lokuvela kwegciwane lomkhuhlane B lihamba kancane kunelo legciwane le-human influenza A.Igciwane loMkhuhlane B lingase futhi libangele izifo zokuphefumula komuntu futhi libangele izifo eziwumshayabhuqe.

I-Adenovirus (AdV) ingeye-mammalian adenovirus, okuyigciwane le-DNA elinemicu ephindwe kabili ngaphandle kwemvilophu.Okungenani ama-genotypes angu-90 atholiwe, angahlukaniswa abe i-AG 7 subgenera.Ukutheleleka kwe-AdV kungabangela izifo ezihlukahlukene, okuhlanganisa inyumoniya, i-bronchitis, i-cystitis, i-eye conjunctivitis, izifo zesisu kanye ne-encephalitis.Inyumoniya ye-Adenovirus ingenye yezinhlobo ezinzima kakhulu zenyumoniya ezitholwa umphakathi ezinganeni, ezibalelwa ku-4% -10% wenyumoniya etholwa umphakathi.

I-Mycoplasma pneumoniae (MP) iwuhlobo lwe-microorganism encane kakhulu ye-prokaryotic, ephakathi kwebhaktheriya negciwane, enokwakheka kwamaseli kodwa alukho udonga lweseli.I-MP ikakhulukazi ibangela ukutheleleka komgudu wokuphefumula womuntu, ikakhulukazi ezinganeni nasebancane.Kungabangela inyumoniya ye-mycoplasma yomuntu, isifo sokuphefumula kwezingane kanye ne-atypical pneumonia.Ukubonakaliswa komtholampilo kuhlukahlukene, iningi lazo ukukhwehlela okukhulu, umkhuhlane, ukugodola, ikhanda elibuhlungu, umphimbo obuhlungu.Ukutheleleka komgudu wokuphefumula ophezulu kanye nenyumoniya ye-bronchial yizona ezivame kakhulu.Ezinye iziguli zingakhula kusukela ekuthelelekeni kwemigudu yokuphefumula ephezulu kuye kunyumoniya enzima, ukucindezeleka kokuphefumula okukhulu nokufa kungase kwenzeke.

I-Respiratory syncytial virus (RSV) igciwane le-RNA, eliyingxenye yomndeni we-paramyxoviridae.Idluliselwa ngamaconsi omoya kanye nokuthintana eduze futhi iyi-pathogen eyinhloko yezifo eziphansi zokuphefumula ezinsaneni.Izinsana ezingenwe i-RSV zingase zibe ne-bronchiolitis enamandla (okubizwa ngokuthi i-bronchiolitis) kanye ne-pneumonia, ehlobene nesifo somoya ezinganeni.Izinsana zinezimpawu ezinzima, ezihlanganisa ukushisa okukhulu, i-rhinitis, i-pharyngitis ne-laryngitis, bese kuba yi-bronchiolitis ne-pneumonia.Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy ne-myocarditis, njll.

Isiteshi

Igama lesiteshi	I-R6 Rection Buffer A	I-R6 Rection Buffer B
FAM	I-SARS-CoV-2	I-HAdV
VIC/HEX	Ukulawula Kwangaphakathi	Ukulawula Kwangaphakathi
CY5	IFV A	MP
I-ROX	IFV B	I-RSV

Imingcele Yezobuchwepheshe

Isitoreji	Uketshezi: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni
Impilo yeshelufu	Uketshezi: izinyanga ezingu-9;I-Lyophilized: izinyanga eziyi-12
Uhlobo lwesifanekiso	Igazi eliphelele, i-Plasma, iSerum
Ct	≤38
CV	≤5.0％
LoD	300Amakhophi/mL
Ukucaciswa	Imiphumela ye-cross-reactivity ibonise ukuthi kwakungekho ukusabela okuphambene phakathi kwekhithi kanye ne-coronavirus yomuntu i-SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, i-rhinovirus A, B, C, i-chlamydia pneumoniae, i-metapneumovirus yomuntu, i-enterovirus A, B, C, D, igciwane le-human pulmonary, i-epstein-barr virus, igciwane lesimungumungwane, igciwane le-human cytomegalo, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kanye ne-cryptococcus esanda kuzalwa kanye ne-nucleic acid yomuntu.
Izinsimbi Ezisebenzayo	Ingakwazi ukufanisa amathuluzi e-PCR e-fluorescent avamile emaketheSLAN-96P Real-Time PCR Systems I-ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems I-QuantStudio^®5 Izinhlelo ze-PCR Zesikhathi Sangempela I-LightCycler^®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System, BioRad Isistimu ye-PCR ye-CFX Opus 96 Yesikhathi Sangempela