Amagciwane Ayisithupha Okuphefumula
Igama lomkhiqizo
I-HWTS-RT175-Six Pathogens Respiratory Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Izifo zokuphefumula ziyiqembu elivame kakhulu lezifo zabantu ezingaba khona kunoma yibuphi ubulili, ubudala kanye nendawo futhi zingenye yezimbangela ezibaluleke kakhulu zokugula nokufa kwabantu emhlabeni wonke. Izifo ezivamile zokuphefumula zokuphefumula zihlanganisa i-respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) kanye ne-Mycoplasma pneumoniae. Izimpawu zomtholampilo nezimpawu ezibangelwa izifo zepheshana lokuphefumula ziyefana, kodwa ukwelashwa, ukusebenza kahle, kanye nobude besifo kuyahlukahluka phakathi kwezifo ezibangelwa amagciwane ahlukene. Njengamanje, izindlela eziyinhloko zokuhlonza amagciwane angenhla okuphefumula elabhorethri zihlanganisa: ukuhlukaniswa kwegciwane, ukutholwa kwe-antigen kanye nokutholwa kwe-nucleic acid. Le khithi isiza ekuxilongweni kwezifo ezibangelwa amagciwane okuphefumula ngokuthola nokuhlonza ama-nucleic acid athile egciwane kubantu abanezimpawu nezimpawu zezifo zokuphefumula, kuhlanganiswe nokunye okutholwe emitholampilo naselabhorethri.
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula ye-Oropharyngeal swab |
| Ct | I-Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| LoD | I-LoD ye-Adv, i-MP, i-RSV, i-hMPV, i-RhV ne-PIV wonke angama-200Copies/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kwe-cross-reactivity ibonise ukuthi akukho-reactivity phakathi kwekhithi kanye ne-coronavirus yenoveli, igciwane lomkhuhlane A, igciwane lomkhuhlane B, i-human bocavirus, i-cytomegalovirus, i-herpes simplex virus uhlobo 1, i-varicella zoster virus, i-EBV, i-pertussis bacillus, i-Chlamydophila phila, i-Corynebacterium coliosis, i-Corynebacterium coliosis fluus Lactobacillus spp, Legionella pneumophila, C. catarrhalis, kanye nezinhlobo ezinciphile ze-Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermicoccus epidermicoccusesptococcus, St. I-Streptococcus salivarius, i-Actinobacillus baumannii, i-Narrow-feeding maltophilic monococci, i-Burkholderia maltophilia, i-Streptococcus striatus, i-Nocardia sp., i-Sarcophaga viscosa, i-Citrobacter citriodora, i-Cryptococcus spp, i-Aspertus, i-Aspertus, i-Aspertus, i-Aspertus, i-Aspertus, i-Aspertus, i-Aspertus, i-Aspertus, i-Aspertus, i-Placis, i-Aspertus flallus I-Candida albicans, i-Rohypnogonia viscera, i-oral streptococci, i-Klebsiella pneumoniae, i-Chlamydia psittaci, i-Rickettsia q fever kanye ne-nucleic acid yomuntu. Amandla okunqanda ukugxambukela: i-mucin (60 mg/mL), igazi lomuntu, i-benfotiamine (2 mg/mL), i-oxymetazoline (2 mg/mL), i-sodium chloride (20 mg/mL), i-beclomethasone (20 mg/mL), i-dexamethasone (20 mg/mL), i-flunitrazolone/lone aceamnomi (20mL), i-flunitrazolone/lone aceamnomi mg/mL), budesonide (1 mg/mL), mometasone (2 mg/mL), fluticasone (2 mg/mL), histamine hydrochloride (5 mg/mL), intranasal live influenza virus vaccine, i-benzocaine (10%), menthol (10%), zanamivir (20 mg/1virpirin/mL) i-oseltamivir (0.15 mg/mL), i-mupirocin (20 mg/mL), i-tobramycin (0.6 mg/mL), i-UTM, i-saline, i-guanidine hydrochloride (5 M/L), i-Tris (2 M/L), i-ENTA-2Na (0.6 M/L), i-trilostane (15%), isopropyl, i-potassium ye-alcohol (2%) kanye ne-alcohol (2%). ukuhlolwa kokuphazamiseka, imiphumela yabonisa ukuthi kwakungekho ukusabela kokuphazanyiswa emiphumeleni yokutholwa kwe-pathogen ekugxilweni okungenhla kwezinto eziphazamisayo. |
| Izinsimbi Ezisebenzayo | SLAN-96P Real-Time PCR Systems I-Applied Biosystems 7500 Real-Time PCR System I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)ngu-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. kunconywa ukukhishwa kwesampula kanye neizinyathelo ezilandelayo kufanele kubeqhubaed ngokuhambisana ngokuqinile ne-IFUkweKit.
