Izifo Eziyisithupha Zokuphefumula
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-RT175-Izifo Eziyisithupha Zokuphefumula Ze-Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Ukutheleleka kokuphefumula kuyiqembu elivame kakhulu lezifo zabantu ezingavela kunoma yiluphi ubulili, ubudala kanye nendawo futhi kungenye yezimbangela ezibaluleke kakhulu zokugula nokufa kwabantu emhlabeni jikelele. Izifo ezivamile zokuphefumula zifaka phakathi igciwane lokuphefumula elihambisanayo, i-adenovirus, i-human metapneumovirus, i-rhinovirus, igciwane le-parainfluenza (I/II/III) kanye ne-Mycoplasma pneumoniae. Izimpawu zomtholampilo ezibangelwa ukutheleleka kwendlela yokuphefumula ziyafana, kodwa ukwelashwa, ukusebenza kahle, kanye nobude besifo kuyahlukahluka phakathi kokutheleleka okubangelwa amagciwane ahlukene. Njengamanje, izindlela eziyinhloko zokuthola amagciwane okuphefumula angenhla elabhorethri zifaka: ukuhlukaniswa kwegciwane, ukutholwa kwe-antigen kanye nokutholwa kwe-nucleic acid. Le khithi isiza ekuxilongweni kokutheleleka kwamagciwane okuphefumula ngokuthola nokuhlonza ama-nucleic acid athile egciwane kubantu abanezimpawu nezimpawu zokutheleleka kokuphefumula, kuhlangene nezinye izinto ezitholwe emitholampilo kanye nelabhorethri.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula ye-oropharyngeal swab |
| Ct | I-Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| I-LoD | I-LoD ye-Adv, i-MP, i-RSV, i-hMPV, i-RhV kanye ne-PIV konke kungamakhophi angu-200/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kokusabela okuxubile ikhombisile ukuthi akukho ukusabela okuxubile phakathi kwekhithi kanye ne-coronavirus entsha, igciwane le-influenza A, igciwane le-influenza B, i-human bocavirus, i-cytomegalovirus, igciwane le-herpes simplex uhlobo 1, igciwane le-varicella zoster, i-EBV, i-pertussis bacillus, i-Chlamydophila pneumoniae, i-Corynebacterium spp, i-Escherichia coli, i-Haemophilus influenzae, i-Lactobacillus spp, i-Legionella pneumophila, i-C. catarrhalis, kanye nezinhlobo ezincishisiwe ze-Mycobacterium tuberculosis, i-Neisseria meningitidis, i-Neisseria spp, i-Pseudomonas aeruginosa, i-Staphylococcus aureus, i-Staphylococcus epidermidis, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Streptococcus salivarius, i-Actinobacillus baumannii, i-Narrow-feeding maltophilic monococci, i-Burkholderia maltophilia, i-Streptococcus striatus, i-Nocardia sp., i-Sarcophaga viscosa, i-Citrobacter citriodora, i-Cryptococcus spp, i-Aspergillus fumigatus, i-Aspergillus flavus, i-Pneumatobacteria spp, i-Candida albicans, i-Rohypnogonia viscera, i-oral streptococci, i-Klebsiella pneumoniae, i-Chlamydia psittaci, i-Rickettsia q fever kanye nama-human genomic nucleic acids. Amandla okulwa nokuphazamiseka: i-mucin (60 mg/mL), igazi lomuntu, i-benfotiamine (2 mg/mL), i-oxymetazoline (2 mg/mL), i-sodium chloride (20 mg/mL), i-beclomethasone (20 mg/mL), i-dexamethasone (20 mg/mL), i-flunitrazolone (20 μg/mL), i-triamcinolone acetonide (2 mg/mL), i-budesonide (1 mg/mL), i-mometasone (2 mg/mL), i-fluticasone (2 mg/mL), i-histamine hydrochloride (5 mg/mL), umuthi wokugomela igciwane lomkhuhlane eliphilayo ngaphakathi kwekhala, i-benzocaine (10%), i-menthol (10%), i-zanamivir (20 mg/mL), i-ribavirin (10 mg/L), i-paramivir (1 mg/mL), i-oseltamivir (0.15 mg/mL), i-mupirocin (20 mg/mL), i-tobramycin (0.6) mg/mL), i-UTM, i-saline, i-guanidine hydrochloride (5 M/L), i-Tris (2 M/L), i-ENTA-2Na (0.6 M/L), i-trilostane (15%), i-isopropyl alcohol (20%), kanye ne-potassium chloride (1 M/L) kuhlolwe ukuphazamiseka, imiphumela ibonise ukuthi akukho ukusabela kokuphazamiseka emiphumeleni yokuthola igciwane ekugxileni okungenhla kwezinto eziphazamisayo. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Uhlelo lwe-PCR lwe-Applied Biosystems 7500 Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)yiJiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kunconywa ukukhishwa kwesampula kanyeizinyathelo ezilandelayo kufanele zibeukuziphathakulandelwe ngokuqinile yi-IFUyeKithi.
