I-Staphylococcus Aureus kanye ne-Staphylococcus Aureus Engamelani ne-Methicillin (MRSA/SA)
Igama lomkhiqizo
I-HWTS-OT062 Staphylococcus Aureus kanye ne-Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Staphylococcus aureus ingenye yamabhaktheriya abalulekile abangela izifo ezibangelwa ukutheleleka kwe-nosocomial. I-Staphylococcus aureus (SA) ingeye-staphylococcus futhi imele amagciwane ane-Gram-positive, angakhiqiza ubuthi obuhlukahlukene kanye nama-enzyme ahlaselayo. Amagciwane anezici zokusabalala kabanzi, ukugula okunamandla kanye nesivinini sokumelana okuphezulu. I-thermostable nuclease gene (nuc) iyi-gene elondolozwe kakhulu ye-staphylococcus aureus.
Isiteshi
| I-FAM | i-mecA gene engamelana ne-methicillin |
| I-ROX | Ukulawula Kwangaphakathi |
| CY5 | i-staphylococcus aureus nuc gene |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ futhi ivikelwe ekukhanyeni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | amasampula okutheleleka kwe-sputum, isikhumba kanye nezicubu ezithambile, kanye namasampula e-nasal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| I-LoD | I-1000 CFU/mL staphylococcus aureus, i-1000 CFU/mL methicillin-resistant bacteria. Uma ikhithi ithola ireferensi ye-LoD kazwelonke, i-1000/mL staphylococcus aureus ingatholakala |
| Ukucaciswa | Ukuhlolwa kokusabela okuxubile kukhombisa ukuthi le khithi ayinayo i-reactivity exubile nezinye izifo zokuphefumula ezifana ne-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, i-pseudomonas aeruginosa, i-escherichia coli, i-klebsiella pneumoniae, i-acinetobacter baumannii, i-proteus mirabilis, i-enterobacter cloacae, i-streptococcus pneumoniae, i-enterococcus faecium, i-candida albicans, i-legionella pneumophila, i-candida parapsilosis, i-moraxella catarrhalis, i-neisseria meningitidis, i-haemophilus influenzae. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Engeza u-200µL we-saline evamile ku-precipitate ecutshungulwayo, bese izinyathelo ezilandelayo kufanele zikhishwe ngokwemiyalelo, futhi ivolumu yokuhlanza enconywayo ingu-80µL.
Inketho 2.
I-Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Faka u-1mL we-saline evamile endaweni evundile ngemva kokuwasha nge-saline evamile, bese uxuba kahle. Faka i-Centrifuge ku-13,000r/min imizuzu emi-5, susa i-supernatant (gcina u-10-20µL we-supernatant), bese ulandela imiyalelo yokukhipha okulandelayo.
I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co., Ltd. Ukukhipha kufanele kwenziwe ngokuqinile ngokwesinyathelo sesi-2 sencwadi yemiyalelo. Kunconywa ukusebenzisa amanzi angenayo i-RNase kanye ne-DNase ukuze kuhlanjululwe ivolumu engu-100µL.
