I-Staphylococcus Aureus kanye ne-Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Igama lomkhiqizo
I-HWTS-OT062 I-Staphylococcus Aureus kanye ne-Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) I-Nucleic Acid Detection Kit(Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Staphylococcus aureus ingenye yebhaktheriya ebalulekile ye-pathogenic ye-nosocomial infection. I-Staphylococcus aureus (SA) ingeye-staphylococcus futhi imele amagciwane e-Gram-positive, angakhiqiza ubuthi obuhlukahlukene nama-enzyme ahlaselayo. Amabhaktheriya anezici zokusabalalisa okubanzi, i-pathogenicity eqinile kanye nezinga eliphezulu lokumelana. I-nuclease gene ye-thermostable (i-nuc) iwufuzo olugcinwe kakhulu lwe-staphylococcus aureus.
Isiteshi
| FAM | Ufuzo lwe-mecA olumelana ne-methicillin |
| I-ROX | Ukulawula Kwangaphakathi |
| CY5 | i-staphylococcus aureus nuc gene |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ futhi ivikelwe ekukhanyeni |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | amasampula okutheleleka kwesikhwehlela, isikhumba nezicubu ezithambile, namasampula e-swab yamakhala |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL amagciwane amelana ne-methicillin. Lapho ikhithi ithola inkomba ye-LoD kazwelonke, i-staphylococcus aureus engu-1000/mL ingatholwa. |
| Ukucaciswa | Ukuhlolwa kwe-cross-reactivity kukhombisa ukuthi le kit ayinakho ukusebenza kabusha okuphambanayo namanye amagciwane okuphefumula anjenge-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, i-pneumoniatoccoccus aeruginosa, i-escherichinella, i-pneumonia, i-escherichiebklea, baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) ka-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ingasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Engeza u-200µL wosawoti ojwayelekile emvuleni ecutshunguliwe, futhi izinyathelo ezilandelayo kufanele zikhishwe ngokuya ngemiyalo, futhi ivolomu yokuhlaziya enconyiwe ingu-80µL.
Inketho yesi-2.
I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-1mL kasawoti ovamile emvuthweni ngemva kokugeza ngosawoti ovamile, bese uxuba kahle. I-Centrifuge ku-13,000r/min imizuzu emi-5, khipha amandla amakhulu (gcina u-10-20µL we-supernatant), bese ulandela imiyalelo yokukhipha okulandelayo.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Nucleic Acid Extraction noma i-Purification Reagent (YDP302) yi-Tiangen Biotech (Beijing) Co.,Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokwesinyathelo sesi-2 sencwadi yeziqondiso. Kuyanconywa ukusebenzisa i-RNase namanzi angenayo i-DNase ukuze uthole ivolumu engu-100µL.
