Ikhithi Yokuthola I-Staphylococcus Aureus kanye ne-Staphylococcus Aureus Nucleic Acid Engamelani ne-Methicillin
Igama lomkhiqizo
Ikhithi Yokuthola I-Staphylococcus Aureus kanye ne-Staphylococcus Aureus Engamelani ne-Methicillin (i-Fluorescence PCR)
Ukusetshenziswa Okuhlosiwe
Leli khithi lisetshenziselwa ukuthola i-staphylococcus aureus kanye ne-staphylococcus aureus nucleic acids engamelana ne-methicillin kumasampula esikhwehlela somuntu, amasampula esikhwehlela samakhala kanye namasampula okutheleleka kwesikhumba nezicubu ezithambile ngaphakathi kwe-vitro. Isampula yesikhwehlela kanye nesampula yesikhwehlela samakhala kusetshenziswa ezikhungweni zezokwelapha ukuvimbela nokulawula ukutheleleka kwe-MRSA nosocomial ezigulini ezilaliswe esibhedlela, okuhlanganisa iziguli ezinakekelwa kakhulu, iziguli ezihlinzwa kanye neziguli ezinakekelwa isikhathi eside; Ingasetshenziswa futhi ekuxilongweni okusizayo kweziguli ezisolisayo ze-Staphylococcus Aureus kanye ne-Methicillin-Resistant Staphylococcus Aureus ezithelelanayo; Noma ngokubambisana nezinye izivivinyo zelebhu ezifana namasiko amagciwane ukusiza ekuxilongweni kokutheleleka kwesikhumba nezicubu ezithambile ze-MRSA/SA. Imiphumela yokuhlolwa ayisetshenziswa ngqo ukuqondisa ukusetshenziswa kwama-antibiotic.
I-Epidemiology
I-Staphylococcus aureus ingenye yamabhaktheriya abalulekile abangela izifo ezibangelwa ukutheleleka kwe-nosocomial. I-Staphylococcus aureus (SA) ingeye-staphylococcus futhi imele amagciwane ane-Gram-positive, angakhiqiza ubuthi obuhlukahlukene kanye nama-enzyme ahlaselayo. Amagciwane anezici zokusabalala kabanzi, ukugula okunamandla kanye nesivinini sokumelana okuphezulu. I-thermostable nuclease gene (nuc) iyi-gene elondolozwe kakhulu ye-staphylococcus aureus.
Eminyakeni yamuva nje, ngenxa yokusetshenziswa kakhulu kwama-hormone kanye namalungiselelo omzimba kanye nokusetshenziswa kabi kwama-antibiotic e-broad-spectrum, ukutheleleka kwe-nosocomial okubangelwa yi-Methicillin-resistant Staphylococcus aureus (MRSA) ku-Staphylococcus kuye kwanda. Izinga elijwayelekile lokutholakala kwe-MRSA likazwelonke lalingu-30.2% ngo-2019 eShayina. I-MRSA ihlukaniswe nge-MRSA ehlobene nokunakekelwa kwempilo (HA-MRSA), i-MRSA ehlobene nomphakathi (CA-MRSA), kanye ne-MRSA ehlobene nemfuyo (LA-MRSA). I-CA-MRSA, i-HA-MRSA, i-LA-MRSA inomehluko omkhulu ku-microbiology, ukumelana namagciwane (isb., i-HA-MRSA ikhombisa ukumelana nemithi eminingi kune-CA-MRSA) kanye nezici zomtholampilo (isb. indawo yokutheleleka). Ngokusho kwalezi zici, i-CA-MRSA kanye ne-HA-MRSA zingahlukaniswa. Kodwa-ke, umehluko phakathi kwe-CA-MRSA kanye ne-HA-MRSA uyancipha ngenxa yokuhamba njalo kwabantu phakathi kwezibhedlela kanye nemiphakathi. I-MRSA imelana nemithi eminingi, ayimelani nje kuphela nama-antibiotic e-β-lactam, kodwa futhi nama-aminoglycosides, ama-macrolides, ama-tetracyclines nama-quinolones ngamazinga ahlukahlukene. Kunezinhlobo ezinkulu zezindawo ezihlukile zamazinga okumelana nemithi kanye nezitayela ezahlukene. I-MRSA ingangena emzimbeni ngamanxeba esikhumba, ama-follicle ezinwele, igazi kanye nezinye izimbangela zokutheleleka okubangelwa ukusha, kanti iziguli ezinezifo zesikhumba kanye nokusha zisengozini enkulu yokutheleleka yi-MRSA. I-pneumonia ingenye yezifo ezivame kakhulu ze-MRSA, futhi ukwelashwa okungafanele kuyimbangela ebalulekile yokufa okuphezulu.
Amapharamitha Obuchwepheshe
| Isitoreji | ≤ -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-sputum, i-nasal swab, isampula yokutheleleka kwesikhumba nezicubu ezithambile, |
| CV | <5.0% |
| I-LoD | 1000 CFU/mL |
| Ukucaciswa | Ukuhlolwa kokusabela okuxubile kukhombisa ukuthi le khithi ayinayo i-reactivity exubile nezinye izifo zokuphefumula ezifana ne-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, i-pseudomonas aeruginosa, i-escherichia coli, i-klebsiella pneumoniae, i-acinetobacter baumannii, i-proteus mirabilis, i-enterobacter cloacae, i-streptococcus pneumoniae, i-enterococcus faecium, i-candida albicans, i-legionella pneumophila, i-candida parapsilosis, i-moraxella catarrhalis, i-neisseria meningitidis, i-haemophilus influenzae. |
| Izinsimbi Ezisebenzayo | Uhlobo I: Izinhlelo ze-Biosystems 7500 Real-Time PCR, Izinhlelo ze-QuantStudio®5 Real-Time PCR, Izinhlelo ze-SLAN-96P Real-Time PCR (Hongshi Medical Technology Co., Ltd.), Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), i-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 Real-Time PCR. Uhlobo II: I-HWTS-EudemonTM AIO800 |
Ukugeleza Komsebenzi
Ama-reagent namathuluzi ayadingeka kodwa awanikezwanga
Uhlobo I
(1) I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) evela ku-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (Ingasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2) Isampula yokuxuba: 4% NaOH noma i-Sputasol evela ku-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Usawoti ojwayelekile.
Uhlobo II:
Ikhithi ye-DNA/RNA Jikelele ye-Macro & Micro-Test (HWTS-3019-8) evela ku-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Izinto ezisetshenziswayo zidinga kodwa azinikezwa:Amathiphu angenayo i-RNase/DNase, Amagilavu alahlwayo, amashubhu e-EP angenayo i-RNase/DNase, imichilo yamashubhu angu-8 ye-PCR.
