I-Staphylococcus aureus ne-methisylococcus-araphylococcus aureus (MRSA / SA)
Igama lomkhiqizo
I-HWTS-OT062 Staphylococcus Aureus ne-Meticillin-Resurast Staphylococcus Auceus (MRSA / SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Staphylococcus aureus ingenye yamagciwane abalulekile we-pathogenic wokutheleleka kweNosocomial. IStaphylococcus Aureus (SA) ingeStaphylococcus futhi ingummeleli wamagciwane anegramu, angakhiqiza ubuthi obuhlukahlukene nama-enzyme ahlaselayo. Amagciwane anezimpawu zokusatshalaliswa okubanzi, i-pathogenicicial eqinile kanye nezinga eliphakeme lokumelana. I-Thermostable Nuclease Gene (NUC) i-gene elondolozwe kakhulu ye-staphylococcus aureus.
Insungubezi
| -Yenza | I-Meca gene gene yohlobo lweMeca |
| Ivupho | Ukulawulwa Kwangaphakathi |
| Cy5 | staphylococcus aureus geneus gene |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ & ivikelwe ukukhanya |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | I-Sputum, isikhumba namasampula wokutheleleka kwezicubu ezithambile, namasampula we-nasal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | I-1000 cfu / ml staphylococcus aureus, ama-bacteria amelana ne-1000 cfu / ml methicillin. Lapho ikhithi ithola ireferensi yeNational Lord, i-1000 / ml staphylococcus aureus ingatholwa |
| Ukucacisa | Ukuhlolwa kwe-Cross-Recisety kukhombisa ukuthi le khithi ayinawo amanye ama-cross recerity namanye ama-pathogen okuphefumula afana ne-methicillin-mictaphylococcus, i-methilillin-aeruginosas epidermidis, i-meticillin-aeruginosa I-Baumannii, i-Proteus Mirabilis, i-Enterobacter Cloacae, i-streptococcus pneumoniae, i-enterococcus albium, i-ligionella parapsosis, i-moraxella catarhalasis, i-neishella meningidis, i-haemophilus yomkhumulo. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-Macro & Micro-Test Genomic DNA / RNA KIT (HWTS-3019) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Ingasetshenziswa nge-macro & micro-3006cractor extractor (HWS-3006C, HWTS- 3006b). Faka i-200μL ye-saline ejwayelekile ku-precipitate esetshenzisiwe, futhi izinyathelo ezilandelayo kufanele zikhishwe ngokuya ngemiyalo, futhi ivolumu yokuqedwa elinconyiwe lingu-80μL.
Inketho 2.
I-Macro & Micro-Test Samputer Reled Reser Referent Reagent (HWTS-3005-8) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd. Faka i-1ml ye-saline ejwayelekile ku-farine evamile, bese uxuba kahle. I-centrifuge ku 13,000r / min imizuzu emi-5, susa ophezulu (ukubhuka ngo-10-20μL we-supernatant), bese ulandela imiyalo yokukhishwa okulandelayo.
I-realent enconyiwe I-realent: Ukukhishwa kwe-nucleic acid noma i-realegent yokuhlanza (yppp02) nguTiangen Biotech (Beijing) Co, Ltd. Ukukhishwa kufanele kwenziwe ngokuqinile ngokuya ngesinyathelo 2 sencwadi yokufundisa. Kunconywa ukusebenzisa amanzi we-rnase ne-dnase-free ukuqedwa ngevolumu ye-100μL.
