I-Ureaplasma Parvum Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-HWTS-UR046-Ureaplasma Parvum Nucleic Acid (i-Fluorescence PCR)
I-Epidemiology
Izinhlobo ze-Ureaplasma ezihlotshaniswa ne-pathogenesis yabantu zihlukaniswe ngamaqembu amabili e-bio kanye nama-serotypes angu-14. I-Biogroup Ⅰ yi-Ureaplasma urealyticum, ehlanganisa ama-serotypes: 2, 4, 5, 7, 8, 9, 10, 11, 12, kanye no-13. I-Biogroup Ⅱ yi-Ureaplasma parvum, ehlanganisa ama-serotypes: 1, 3, 6, 14. I-Ureaplasma iyi-parasite evamile noma i-commensal endleleni yokuzala yabesifazane engezansi futhi ingenye yezifo ezibalulekile ezibangela izifo ezithathelwanayo ohlelweni lwe-genitourinary. Ngaphezu kokubangela ukutheleleka kwe-genitourinary tract, abesifazane abanokutheleleka kwe-Ureaplasma nabo banamathuba amaningi okudlulisela i-pathogen kubalingani babo bocansi. Ukutheleleka kwe-Ureaplasma nakho kungenye yezimbangela ezibalulekile zokungazali. Uma abesifazane abakhulelwe betheleleke nge-Ureaplasma, lokhu kungase kuholele nasekuqhekekeni kwama-membrane ngaphambi kwesikhathi, ukubeletha ngaphambi kwesikhathi, isifo sokucindezeleka kokuphefumula kwezingane ezisanda kuzalwa, ukutheleleka kwangemva kokubeletha kanye neminye imiphumela emibi yokukhulelwa, edinga ukunakwa okukhulu.
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | umgudu womchamo wabesilisa, umgudu wokuzala wabesifazane |
| Ct | ≤38 |
| CV | ＜5.0% |
| I-LoD | Amakhophi angu-400/mL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I: Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), kanye ne-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (engasetshenziswa ne-EudemonTM I-AIO800 (HWTS-EQ007)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Umthamo wesampula okhishwe ungama-200μL kanti umthamo wokulungisa onconywayo ungama-150μL.
