I-Nile Virus Nucleic Acid
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid Yegciwane Le-HWTS-FE041-West Nile Virus (i-Fluorescence PCR)
I-Epidemiology
Igciwane laseWest Nile liyilungu lomndeni iFlaviviridae, uhlobo lweFlavivirus, futhi lisesigabeni esifanayo negciwane laseJapane i-encephalitis, igciwane le-dengue, igciwane le-yellow fever, igciwane le-St. Louis encephalitis, igciwane le-hepatitis C, njll. Eminyakeni yamuva nje, i-West Nile fever ibangele ubhubhane eNyakatho Melika, eYurophu, eMpumalanga Ephakathi, naseNingizimu-mpumalanga ye-Asia, futhi isibe yisifo esithelelanayo esikhulu kunazo zonke esithinta i-United States njengamanje. Igciwane laseWest Nile lidluliselwa ngezinyoni njengezindawo zokugcina amanzi, futhi abantu batheleleka ngokulunywa omiyane abadla izinyoni (ama-ornithophilic) njengeCulex. Abantu, amahhashi, nezinye izilwane ezincelisayo bayagula ngemva kokulunywa omiyane abatheleleke ngegciwane laseWest Nile. Amacala amancane angase avele nezimpawu ezifana nomkhuhlane njengomkhuhlane kanye nekhanda elibuhlungu, kuyilapho amacala amakhulu angase avele nezimpawu zesistimu yezinzwa ephakathi noma ngisho nokufa [1-3]. Eminyakeni yamuva nje, ngenxa yokujula kokushintshana kwamazwe ngamazwe kanye nokubambisana, ukushintshana phakathi kwamazwe kuye kwaba njalo, futhi inani labahambi liye landa unyaka nonyaka. Ngesikhathi esifanayo, ngenxa yezici ezifana nokufuduka kwezinyoni ezifudukayo, amathuba okuthi umkhuhlane we-West Nile ungeniswe eShayina akhuphukile[4].
Amapharamitha Obuchwepheshe
| Isitoreji | -18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | amasampula e-serum |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Izinsimbi Ezisebenzayo | Kusebenza ku-reagent yokuthola uhlobo I:Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time, I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5, Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.), Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Uhlelo lwe-BioRad CFX96 lwe-Real-Time PCR, Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR. Kusebenza ku-reagent yokuthola uhlobo lwesibili: I-EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza Komsebenzi
Inketho 1.
I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Nucleic Acid Extractor (HWTS-3006).
Inketho 2.
I-reagent yokukhipha enconywayo: I-Nucleic Acid Extraction noma i-Purification Kit (YD315-R) eyenziwe yi-Tiangen Biotech (Beijing) Co., Ltd.
