I-West Nile Virus Nucleic Acid
Igama lomkhiqizo
I-HWTS-FE041-West Nile Virus Nucleic Acid Detection Kit(Fluorescence PCR)
I-Epidemiology
I-West Nile virus iyilungu lomndeni i-Flaviviridae, uhlobo lwe-Flavivirus, futhi isohlwini olufanayo ne-Japanese encephalitis virus, i-dengue virus, i-yellow fever virus, i-St. Louis encephalitis virus, igciwane le-hepatitis C, njll. Igciwane le-West Nile lisakazwa ngezinyoni njengama-reservoir host, futhi abantu batheleleka ngokulunywa omiyane abadla izinyoni (ornithophilic) njengeCulex. Abantu, amahhashi, nezinye izilwane ezincelisayo ziyagula ngemva kokulunywa omiyane abangenwe igciwane laseWest Nile. Izimo ezithambile zingase zibe nezimpawu ezinjengomkhuhlane ezifana nomkhuhlane kanye nekhanda elibuhlungu, kuyilapho izimo ezinzima zingase zibe nezimpawu zesistimu yezinzwa ezimaphakathi noma ngisho nokufa[1-3]. Eminyakeni yamuva nje, ngenxa yokujula kokuhwebelana nokubambisana kwamazwe ngamazwe, ukushintshana phakathi kwamazwe kuye kwaba njalo, futhi inani labahambi liye landa unyaka nonyaka. Ngesikhathi esifanayo, ngenxa yezici ezifana nokufuduka kwezinyoni ezifudukayo, amathuba okuba i-West Nile fever yethulwe eShayina ayanda[4].
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | amasampula e-serum |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/μL |
| Izinsimbi Ezisebenzayo | Isebenza ekuthayipheni i-reagent yokuthola i-I: I-Applied Biosystems 7500 Real-Time PCR Systems, I-QuantStudio®5 Amasistimu e-PCR wesikhathi sangempela, I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), I-LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ubuchwepheshe be-Hangzhou Bioer), I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), I-BioRad CFX96 Real-Time PCR System, I-BioRad CFX Opus 96 Real-Time PCR System. Isebenza ohlotsheni lwe-II yokutholwa kwe-reagent: EudemonTMI-AIO800 (HWTS-EQ007) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Nucleic Acid Extractor(HWTS-3006).
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokumba esinconyiwe: I-Nucleic Acid Extraction noma Ikhithi Yokuhlanza(YD315-R) ekhiqizwa i-Tiangen Biotech(Beijing) Co., Ltd.
