Igciwane le-Zaire Ebola
Igama lomkhiqizo
I-HWTS-FE008 Zaire Ebola virus nbobula nucleic acid ukutholwa kokutholwa (i-fluorescence PCR)
I-Epidemiology
Igciwane le-Ebola lingelikaFiloviridae, okuyigciwane elingenamikhawulo elibi le-RNA elingezindliwe. Amagciwane ama-filaments amade ane-viruon yobude obungu-1000mm nobubanzi obungama-100nm. I-Ebola Virus Genome iyi-rna engemihle encedmed enobukhulu engu-18.9kb, efaka amaprotheni angu-7 wesakhiwo kanye namaprotheni ayi-1 angahlelekile. Igciwane le-Ebola lingahlukaniswa ngezinhlobo ezinjengeZaire, eSudan, iBundibugyo, ihlathi leTai kanye neReron. Phakathi kwazo, uhlobo lwe-zaire nohlobo lweSudan kubikwe ukuthi kubangela ukushona kwabantu abaningi ekuthelelekeni. I-EHF (Ebola Hemorrhagic Fever) yisifo esibuhlungu se-hemorrhagic esidalwa yigciwane le-Ebola. Abantu ikakhulukazi bangenwa yile ngculazi ngoketshezi lomzimba, amabhulukwe kanye ne-excreta yeziguli noma izilwane ezinegciwane, kanye nokubonakaliswa kwemitholampilo ikakhulukazi ukulimala komkhuhlane, ukopha kanye nokulimala kwezitho eziningi. I-EHF inesilinganiso esiphezulu sokufa kuka-50% -90%.
Insungubezi
| -Yenza | I-MP nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤-18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-9 |
| Uhlobo lwe-Specimen | I-serum entsha, i-plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| Indlu edayisa insimbi | Amakhophi angama-500 / μL |
| Ukucacisa | Sebenzisa ama-kits ukuvivinya izinkomba ezingezinhle zenkampani, imiphumela ihlangabezana nezidingo. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®5 Systems real-time PCREMS ISlan-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®480 Uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR, kanye ne-Biorad CFX Opus 96 Uhlelo lwe-PRCR lwangempela lwe-Real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-realent enconyiwe ye-realent: I-QAAMP Viral RNA MINI KIT (52904), isizinda se-nucleic acid noma i-reagent yokuhlanza (ypp15-R) nguTiangen Biotech (Beijing) Co, Ltd. Kufanele kukhishwe ngokuqinile ngokuya ngemiyalo, futhi umthamo wokukhishwa onconyiwe wesampula u-140μL futhi ivolumu yokuqedwa enconyiwe ingu-60μL.
Inketho 2.
I-realent enconyiwe I-realent: Macro & Micro-Vival DNA / RNA KIT (HWTS-3004-32, HWS-3004-48, ne-MACRE-3004-96) ne-MACRO-3004-9 (HWS-3006) .it kufanele ikhishwe ngokuya ngemiyalo. Ivolumu yesampula ye-Extraction ingu-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-80μL.
Inketho 3.
I-reagents yokukhishwa enconyiwe: I-Nucleic Acid Extraction realent (1000020261) kanye nohlelo oluphezulu lokulungiselela isampula (i-MGISP-960) nge-BGI kufanele ikhishwe ngokuya ngemiyalo. IVolumu yokukhishwa yi-160μL, futhi ivolumu yokuqedwa enconyiwe ingu-60μL.
