Igciwane le-Ebola laseZaire
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-Zaire ye-HWTS-FE008 (i-Fluorescence PCR)
I-Epidemiology
Igciwane le-Ebola lingelaseFiloviridae, okuyigciwane le-RNA elingenayo imigqa eyodwa elingahlukaniswanga. Ama-virus ayimicu emide enobude obumaphakathi be-virion obungu-1000nm kanye nobubanzi obungu-100nm. I-genome yegciwane le-Ebola iyi-RNA engenayo imigqa engahlukaniswanga enobukhulu obungu-18.9kb, equkethe amaprotheni ayi-7 ahlelekile kanye neprotheni eyodwa engeyona eyakhekile. Igciwane le-Ebola lingahlukaniswa ngezinhlobo ezifana neZaire, Sudan, Bundibugyo, Tai Forest kanye neReston. Phakathi kwazo, uhlobo lwe-Zaire kanye nohlobo lwe-Sudan kubikwe ukuthi lubangela ukufa kwabantu abaningi ngenxa yokutheleleka. I-EHF (Ebola Hemorrhagic Fever) yisifo esithathelwanayo esibangelwa ukopha okubukhali esibangelwa yigciwane le-Ebola. Abantu batheleleka kakhulu ngokuthintana noketshezi lomzimba, uketshezi kanye nendle yeziguli noma izilwane ezithelelekile, futhi izimpawu zemitholampilo ikakhulukazi umkhuhlane ophumayo, ukopha kanye nomonakalo wezitho eziningi. I-EHF inezinga eliphezulu lokufa elingu-50%-90%.
Isiteshi
| I-FAM | i-asidi ye-nucleic ye-MP |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-9 |
| Uhlobo Lwesifanekiso | I-serum entsha, i-Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| I-LoD | Amakhophi angu-500/μL |
| Ukucaciswa | Sebenzisa amakhithi ukuhlola izinkomba ezingezinhle zenkampani, imiphumela iyahlangabezana nezidingo. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time Izinhlelo ze-PCR ze-QuantStudio®5 Zesikhathi Sangempela Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR, kanye nohlelo lwe-BioRad CFX Opus 96 Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-reagent yokukhipha enconyiwe: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd. Kufanele ikhishwe ngokuhambisana nemiyalelo, futhi ivolumu yokukhipha enconyiwe yesampula ingu-140μL kanti ivolumu yokususa enconyiwe ingu-60μL.
Inketho 2.
I-reagent yokukhipha enconywayo: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kufanele ikhishwe ngokwemiyalelo. Umthamo wesampula yokukhipha ungu-200μL, kanti umthamo wokususa onconywayo ungu-80μL.
Inketho 3.
Ama-reagent okukhipha anconyiwe: I-Nucleic Acid Extraction Reagent (1000020261) kanye ne-High Throughput Automated Sample Preparation System (MGISP-960) yi-BGI kufanele ikhishwe ngokwemiyalelo. Umthamo wokukhipha ungu-160μL, kanti umthamo wokususa onconyiwe ungu-60μL.
