I-Zaire Ebola Virus
Igama lomkhiqizo
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Igciwane le-Ebola lingelikaFiloviridae, okuyigciwane le-RNA elinomucu owodwa ongahlukaniswanga.Amagciwane ayimicu emide enobude obujwayelekile be-virion obuyi-1000nm kanye nobubanzi obuyi-100nm.I-genome yegciwane le-Ebola iyi-RNA ene-negative-strand engahlukanisiwe enosayizi ongu-18.9kb, efaka ikhodi yamaphrotheni esakhiwo angu-7 kanye nephrotheni eyodwa engahlelekile.Igciwane le-Ebola lingahlukaniswa ngezinhlobo ezifana neZaire, Sudan, Bundibugyo, Tai Forest kanye neReston.Phakathi kwazo, uhlobo lweZaire kanye nohlobo lwaseSudan kubikwe ukuthi zibangele ukufa kwabantu abaningi ngenxa yokutheleleka.I-EHF (Ebola Hemorrhagic Fever) isifo esithathelwanayo esopha kakhulu esibangelwa igciwane le-Ebola.Abantu batheleleka kakhulu ngokuthintana noketshezi lomzimba, uketshezi kanye nendle yeziguli noma izilwane ezingenwe yilesi sifo, futhi ukubonakaliswa kwemitholampilo ikakhulukazi imfiva ephumela ngaphandle, ukopha kanye nokulimala kwezitho eziningi.I-EHF inezinga eliphezulu lokufa lika-50% -90%.
Isiteshi
| FAM | MP nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ |
| Impilo yeshelufu | 9 izinyanga |
| Uhlobo Lwesifanekiso | I-serum entsha, i-Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Amakhophi/μL |
| Ukucaciswa | Sebenzisa amakhithi ukuhlola izinkomba ezingezinhle zenkampani, imiphumela ihlangabezana nezidingo. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems I-SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) I-BioRad CFX96 Real-Time PCR System, kanye ne-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co.,Ltd.Kufanele ikhishwe ngokuhambisana ngokuqinile nemiyalelo, futhi umthamo onconywayo wokukhipha isampula ngu-140μL futhi ivolumu ye-elution enconyiwe ngu-60μL.
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). kufanele ikhishwe ngokulandela imiyalelo.Ivolumu yesampula yokukhipha ingu-200μL, futhi ivolumu ye-lution enconyiwe ingu-80μL.
Inketho yesi-3.
Ama-reagents okukhipha anconyiwe: I-Nucleic Acid Extraction Reagent (1000020261) kanye ne-High Throughput Automated Sample Preparation System(MGISP-960) yi-BGI kufanele ikhishwe ngokuya ngemiyalelo.Ivolumu yokukhipha ingu-160μL, futhi ivolumu ye-elution enconyiwe ingu-60μL.
