Igciwane le-zika
Igama lomkhiqizo
I-HWTS-FE002 ZIKA Virus nucleic acid detection detection
Isitifiketi
CE
I-Epidemiology
Igciwane le-Zika lingelohlobo lwe-genus flaviviridae, iyigciwane le-rna elihlangene elilodwa elihlanjululwe line-rna elihlangene nelingu-40-70nm. Inomvilophu, iqukethe ama-nucleotides ayi-10794, futhi afake ama-amino acid angama-3419. Ngokusho kwe-genotype, ihlukaniswe ngohlobo lwe-Afrika nohlobo lwase-Asia. Isifo segciwane se-Zika yiSifo esizikhawulela izifo ezithathelwanayo ezibangelwa yi-zika virus, edluliselwa ngokukhethekile ngokulunywa ngomiyane we-AEDES AEGYPTTI. Izici zemitholampilo ikakhulukazi umkhuhlane, i-rash, i-arthralgia noma i-conjunctivitis, futhi akuvamile ukufa. Ngokwe-World Health Organisation, i-Micro Ceryphaly ne-Guillain-Barre (Guillain-Barrome) ingahle ihlotshaniswe nokutheleleka ngegciwane le-zika.
Insungubezi
| -Yenza | UZika Virus nucleic acid |
| Ivupho | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | ≤30 ℃ & kuvikelwe ukukhanya |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | i-serum entsha |
| Ct | ≤38 |
| CV | <5.0% |
| Indlu edayisa insimbi | 500copies / ml |
| Ukucacisa | Sebenzisa ikhithi ukuthola amasampula e-serum nge-zika virus negative, futhi imiphumela ayilungile. Imiphumela yokuhlola ukuphazamiseka ikhombisa ukuthi lapho ukugcwala kwe-bilirubin ku-serum kungabi ngaphezu kwe-168.2μmol / ml, i-hemoglobin okuhlushwa okukhiqizwa yi-hemolysis akuphezulu kwe-130mmol Ukuhlushwa ku-serum akukubi ngaphezu kwe-5mg / ml, akukho mthelela egciwane le-dengue, igciwane le-zika noma Ukutholwa kwegciwane le-chikungunya. I-Hepatitis I-Virus, i-Hepatitis B Virus, i-Hepatitis C Virus, i-herpes virus, i-Eastern, i-Bunya virus, amasampula e-West Nile kanye ne-Suman genomic serum akhethiwe ukuhlolwa kwe-cross-genomic serum, futhi imiphumela ikhombisa ukuthi akukho ukusabela kwe-cross phakathi kwale kit kanye namagciwane ashiwo ngenhla. |
| Izinsimbi ezisebenzayo | I-ABI 7500 real-time PCRS SystemsI-ABI 7500 Fast Real-Time Pcr Systems I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 Uhlelo lwe-PCR lwe-Real-time real I-LineGene 9600 Plus Real-Time Pcc Retection System I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-QAAMP i-RNA mini kit (52904), isizinda se-nucleic acid noma i-reagent yokuhlanza (YI-D315-R) ngeTiangen Biotech (Beijing) Co, Ltd.UkukhishwaKufanele ikhishwe ngokuya ngemiyalo yokukhishwa, futhi ivolumu yokukhishwa enconyiwe ingu-140 μL futhi ivolumu yokuqedwa elinconyiwe lingu-60 μL.
Inketho 2.
I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3004-32, HWTS-3004-48, HWS-3004-96) ne-Macro & Micro-3006). Ukukhishwa kufanele kukhishwe ngokuya ngemiyalo. Ivolumu yesampula ye-Extraction ingu-200
