Igciwane leZika
Igama lomkhiqizo
I-HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Igciwane leZika liyingxenye yohlobo lwe-Flaviviridae, igciwane le-RNA elinemucu owodwa elinemicu eyodwa enobubanzi obungu-40-70nm. Inemvilophu, iqukethe ama-nucleotide angu-10794, futhi ifaka ama-amino acid angu-3419. Ngokusho kwe-genotype, ihlukaniswe ngohlobo lwase-Afrika kanye nohlobo lwase-Asia. Isifo se-Zika virus yisifo esithathelwanayo esizikhawulelayo esibangelwa igciwane leZika, elisakazwa kakhulu ngokulunywa omiyane i-Aedes aegypti. Izici zomtholampilo ikakhulukazi imfiva, ukuqubuka, i-arthralgia noma i-conjunctivitis, futhi akuvamile ukuthi ibulale. Ngokusho kwe-World Health Organization, i-microcephaly esanda kuzalwa kanye ne-Guillain-Barre syndrome (i-Guillain-Barré syndrome) ingase ihlotshaniswe nokutheleleka kwegciwane le-Zika.
Isiteshi
| FAM | I-Zika virus nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤30℃ futhi ivikelwe ekukhanyeni |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500Amakhophi/mL |
| Ukucaciswa | Sebenzisa ikhithi ukuthola amasampula e-serum ane-Zika virus negative, futhi imiphumela iba mibi. Imiphumela yokuhlolwa kokugxambukela ibonisa ukuthi lapho ukugcwala kwe-bilirubin ku-serum kungengaphezu kuka-168.2μmol/ml, ukuhlushwa kwe-hemoglobin ekhiqizwa yi-hemolysis akukho ngaphezu kwe-130g/L, ukuhlushwa kwe-lipid yegazi akukho ngaphezu kuka-65mmol/ml, inani eliphelele le-IgG ku-serum alikho ngaphezu kuka-5mg/mL, akukho mphumela wokutholwa kwegciwane i-dengue, igciwane le-chikungue. Igciwane le-Hepatitis A, igciwane le-Hepatitis B, igciwane le-Hepatitis C, i-Herpes virus, i-Eastern equine encephalitis virus, i-Hantavirus, igciwane le-Bunya, igciwane le-West Nile kanye namasampula e-genomic serum yabantu akhethiwe ukuze kuhlolwe i-cross-reactivity, futhi imiphumela ibonisa ukuthi akukho ukusabela okuphambene phakathi kwale kit kanye namagciwane ashiwo ngenhla. |
| Izinsimbi Ezisebenzayo | I-ABI 7500 Real-Time PCR SystemsABI 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-QIAamp Viral RNA Mini Kit(52904), i-Nucleic Acid Extraction noma i-Purification Reagent(YDP315-R) yi-Tiangen Biotech(Beijing) Co.,Ltd.Isizindakufanele ikhishwe ngokulandela imiyalelo yokukhipha, futhi ivolumu yokukhipha enconyiwe ingu-140 μL futhi ivolumu ye-elution enconyiwe ingu-60 μL.
Inketho yesi-2.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhishwa kufanele kukhishwe ngokulandela imiyalelo. Ivolumu yesampula yokukhipha ingu-200 μL, futhi ivolumu ye-elution enconyiwe ingu-80μL.
