Igciwane leZika
Igama lomkhiqizo
Ikhithi Yokuthola I-Nucleic Acid ye-HWTS-FE002 Zika Virus (i-Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Igciwane le-Zika lingelasehlobo lwe-Flaviviridae, ligciwane le-RNA elinezintambo ezinezintambo eyodwa elinobubanzi obungu-40-70nm. Linomkhawulo, liqukethe ama-nucleotide angu-10794, futhi lifaka ama-amino acid angu-3419. Ngokusho kohlobo lwe-genotype, lihlukaniswe ngohlobo lwase-Afrika nolwase-Asia. Isifo segciwane le-Zika yisifo esithathelwanayo esizikhawulela esibangelwa yigciwane le-Zika, esidluliselwa kakhulu ngokulunywa omiyane be-Aedes aegypti. Izici zezokwelapha ikakhulukazi umkhuhlane, ukuqubuka, i-arthralgia noma i-conjunctivitis, futhi akuvamile ukubulala. Ngokusho kwe-World Health Organisation, i-microcephaly yezingane ezisanda kuzalwa kanye ne-Guillain-Barre syndrome (i-Guillain-Barré syndrome) zingase zihlotshaniswe nokutheleleka yigciwane le-Zika.
Isiteshi
| I-FAM | I-nucleic acid yegciwane le-Zika |
| I-ROX | Ukulawula Kwangaphakathi |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤30℃ futhi ivikelwe ekukhanyeni |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | i-serum entsha |
| Ct | ≤38 |
| CV | <5.0% |
| I-LoD | Amakhophi angu-500/mL |
| Ukucaciswa | Sebenzisa ikhithi ukuthola amasampula e-serum anegciwane le-Zika elibi, kanti imiphumela ayibi. Imiphumela yokuhlolwa kokuphazamiseka ikhombisa ukuthi lapho ukugcwala kwe-bilirubin ku-serum kungaphezu kuka-168.2μmol/ml, ukugcwala kwe-hemoglobin okukhiqizwa yi-hemolysis akungaphezu kuka-130g/L, ukugcwala kwamafutha egazini akungaphezu kuka-65mmol/ml, ukugcwala okuphelele kwe-IgG ku-serum akungaphezu kuka-5mg/mL, akukho mphumela egciwaneni le-dengue, ekutholakaleni kwegciwane le-Zika noma igciwane le-chikungunya. Igciwane le-Hepatitis A, igciwane le-Hepatitis B, igciwane le-Hepatitis C, igciwane le-Herpes, igciwane le-Eastern equine encephalitis, i-Hantavirus, igciwane le-Bunya, igciwane le-West Nile kanye namasampula e-serum ye-genomic yabantu akhethwa ukuze kuhlolwe ukuphindaphindwa, futhi imiphumela ikhombisa ukuthi akukho ukusabela okuphambene phakathi kwale kithi kanye namagciwane okukhulunywe ngawo ngenhla. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-ABI 7500 Zesikhathi SangempelaIzinhlelo ze-PCR ze-ABI 7500 ze-Fast Real-Time I-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-QIAamp Viral RNA Mini Kit(52904), i-Nucleic Acid Extraction noma i-Purification Reagent(YDP315-R) yiTiangen Biotech(Beijing) Co.,Ltd.Ukukhishwakufanele kukhishwe ngokwemiyalelo yokukhipha, futhi ivolumu yokukhipha enconywayo ingu-140 μL kanti ivolumu yokususa enconywayo ingu-60 μL.
Inketho 2.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ukukhishwa kufanele kukhishwe ngokwemiyalelo. Umthamo wesampula yokukhishwa ungama-200 μL, kanti umthamo wokususa onconywayo ungama-80μL.
