Igciwane leZika
Igama lomkhiqizo
I-HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
Igciwane leZika liyingxenye yohlobo lwe-Flaviviridae, igciwane le-RNA elinemucu owodwa elinemicu eyodwa enobubanzi obungu-40-70nm.Inemvilophu, iqukethe ama-nucleotide angu-10794, futhi ifaka ama-amino acid angu-3419.Ngokusho kwe-genotype, ihlukaniswe ngohlobo lwase-Afrika kanye nohlobo lwase-Asia.Isifo se-Zika virus yisifo esithathelwanayo esizikhawulelayo esibangelwa igciwane leZika, elisakazwa kakhulu ngokulunywa omiyane i-Aedes aegypti.Izici zomtholampilo ikakhulukazi imfiva, ukuqubuka, i-arthralgia noma i-conjunctivitis, futhi akuvamile ukuthi ibulale.Ngokusho kwe-World Health Organization, i-microcephaly esanda kuzalwa kanye ne-Guillain-Barre syndrome (i-Guillain-Barré syndrome) ingase ihlotshaniswe nokutheleleka kwegciwane le-Zika.
Isiteshi
| FAM | I-Zika virus nucleic acid |
| I-ROX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤30℃ futhi ivikelwe ekukhanyeni |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | i-serum entsha |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 ng/μL |
| Ukucaciswa | Imiphumela yokuhlolwa etholwe yile khithi ngeke ithintwe i-hemoglobin (<800g/L), i-bilirubin (<700μmol/L), kanye ne-blood lipids/ triglycerides (<7mmol/L) egazini. |
| Izinsimbi Ezisebenzayo | I-ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 uhlelo lwe-PCR yesikhathi sangempela I-LineGene 9600 Plus Real-Time PCR Detection System I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
I-QIAamp Viral RNA Mini Kit(52904), i-Nucleic Acid Extraction noma i-Purification Reagent(YDP315-R) yi-Tiangen Biotech(Beijing) Co.,Ltd.Isizindakufanele ikhishwe ngokulandela imiyalelo yokukhipha, futhi ivolumu yokukhipha enconyiwe ingu-140 μL futhi ivolumu ye-elution enconyiwe ingu-60 μL.
Inketho yesi-2.
I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Ukukhishwa kufanele kukhishwe ngokulandela imiyalelo.Ivolumu yesampula yokukhipha ingu-200 μL, futhi ivolumu ye-elution enconyiwe ingu-80μL.
