Izinhlobo eziyi-12 ze-Respiratory Pathogen
Igama lomkhiqizo
I-HWTS-RT071A Izinhlobo eziyi-12 ze-Respiratory Pathogen Nucleic Acid Detection Kit (i-Fluorescence PCR)
Isiteshi
| Isiteshi | hu12I-Reaction Buffer A | hu12I-Reaction Buffer B | hu12I-Reaction Buffer C | hu12I-Reaction Buffer D |
| I-FAM | I-SARS-CoV-2 | I-HADV | I-HPIV Ⅰ | I-HRV |
| I-VIC/HEX | Ukulawula Kwangaphakathi | Ukulawula Kwangaphakathi | I-HPIV Ⅱ | Ukulawula Kwangaphakathi |
| CY5 | I-IFV A | MP | I-HPIV Ⅲ | / |
| I-ROX | I-IFV B | I-RSV | I-HPIV Ⅳ | I-HMPV |
Amapharamitha Obuchwepheshe
| Isitoreji | ≤-18℃ |
| Isikhathi sokuphelelwa yisikhathi | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | I-Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| I-LoD | I-SARS-CoV-2: Amakhophi angu-300/mLIgciwane le-influenza B: Amakhophi angu-500/mLIgciwane le-influenza A: Amakhophi angu-500/mL I-Adenovirus: Amakhophi angu-500/mL i-mycoplasma pneumoniae: Amakhophi angu-500/mL igciwane lokuphefumula le-syncytial: Amakhophi angu-500/mL, igciwane le-parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ): Amakhophi angu-500/mL Igciwane le-Rhinovirus: Amakhophi angu-500/mL i-metapneumovirus yabantu: amakhophi angu-500/mL |
| Ukucaciswa | Ucwaningo lwe-cross-reactivity lubonisa ukuthi akukho ukuhlangana phakathi kwale kit kanye ne-enterovirus A, B, C, D, igciwane le-epstein-barr, igciwane le-measles, i-human cytomegalovirus, i-rotavirus, i-norovirus, igciwane le-mumps, igciwane le-varicella-herpes zoster, i-bordetella pertussis, i-streptococcus pyogenes, i-mycobacterium tuberculosis, i-aspergillus fumigatus, i-candida albicans, i-candida glabrata, i-pneumocystis jirovecii, i-cryptococcus neoformans kanye ne-human genomic nucleic acid. |
| Izinsimbi Ezisebenzayo | Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-TimeIzinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-TimeI-QuantStudio®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR ze-SLAN-96P Zesikhathi Sangempela (i-Hongshi Medical Technology Co., Ltd.) I-LightCycler®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480, Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR (FQD-96A, ubuchwepheshe be-Hangzhou Bioer) I-MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR |
Ukugeleza Komsebenzi
Inketho 1.
I-reagent yokukhipha enconyiwe: I-Reagent Yokukhipha Isampula Ye-Macro & Micro-Test (HWTS-3005-8) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Ukukhipha kufanele kwenziwe ngokuhambisana nemiyalelo.
Inketho 2.
I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ukukhishwa kufanele kwenziwe ngokwemiyalelo eqinile. Ivolumu yokususa enconyiwe ingu-80μL.
Inketho 3.
I-reagent yokukhipha enconyiwe: I-Nucleic Acid Extraction noma i-Purification Kit (YDP315) yi-Tiangen Biotech(Beijing) Co.,Ltd., ukukhishwa kufanele kwenziwe ngokwemiyalelo eqinile. Umthamo wokususa onconyiwe ungu-100μL.
