Izinhlobo eziyi-12 ze-pathogen yokuphefumula
Igama lomkhiqizo
I-HWTS-RT071A 12 izinhlobo ze-pathogen pathogen nucleic acid detection detection (Fluorescence PCR)
Insungubezi
Insungubezi | Hu12Ukuphendula Buffer a | Hu12Ukuphendula Buffer B | Hu12I-Reaction Buffer C | Hu12I-Reaction Buffer d |
-Yenza | I-SARS-CoV-2 | Hadv | Hpiv ⅰ | Hrv |
VIC / Hex | Ukulawulwa Kwangaphakathi | Ukulawulwa Kwangaphakathi | Hpiv ⅱ | Ukulawulwa Kwangaphakathi |
Cy5 | IFV a | MP | Hpiv ⅲ | / |
Ivupho | IFV B | I-RSV | Hpiv ⅳ | I-HMPVV |
Amapharamitha Ezobuchwepheshe
Ukubeka | ≤-18 ℃ |
Ishalofu-impilo | Izinyanga eziyi-12 |
Uhlobo lwe-Specimen | I-Oropharyngengeal Swapy |
Ct | ≤38 |
CV | ≤5.0% |
Indlu edayisa insimbi | I-SARS-CoV-2: 300 Amakhophi / mlumkhuhlane b virus: amakhophi angama-500 / mlUmkhuhlane Igciwane: Amakhophi angama-500 / ml I-Adenovirus: amakhophi angama-500 / ml I-Mycoplasma Pneumoniae: Amakhophi angama-500 / ml Igciwane lokuphefumula lokuvumelanisa: amakhophi angama-500 / ml, Igciwane le-ParainFluenza (ⅰ, ⅱ, ⅲ, ⅳ): Amakhophi angama-500 / ml I-Rhinovirus: Amakhophi angama-500 / ml I-Metapneumovirus yomuntu: Amakhophi angama-500 / ml |
Ukucacisa | Ucwaningo Olusebenzisa Ngokusebenza Ngobumbano lukhombisa ukuthi akukho mpilo yokusebenzisana phakathi kwale khithi ne-entovirus a, b, c, c, c, d, igciwane le-epsterin -br, i-norovirus, igciwane le-mumps, i-varicella-herpes zoster virus, I-Bordetella Pertussis, i-streptococcus pyogene, i-mycobacterium Isifo sofuba, i-aspergillus fumigatus, ama-allicans we-candida, i-candida glabrata, pneumocystis jirovecii, ama-cryptocystis neoformans kanye ne-nucleic yount genomic nucleic acid. |
Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMSKusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCRI-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems (Hongshi Technology Con Technology Co, Ltd.) I-Lightcycler®Uhlelo lwe-480 real-time PCR, I-LineGene 9600 Plus Plus real-time PCCRECT SYPELY (FQD-96A, Hangzhou Bioer Technology) I-MA-6000 Real-Time Cycler Cycler yesikhathi sangempela (Suzhou Molarray Co, Ltd.) I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
Inketho 1.
I-reagent enconyelwe i-reagent: I-Macro & Micro-Test Samputer Reled Reled Reagent (HWTS-3005-8) nguJiangsu Macro & Micro-Test Med-Tech Co, Ltd., Ukukhishwa kufanele kwenziwe ngokuqinile ngemiyalo.
Inketho 2.
I-realent enconyiwe: I-Macro & Micro-Test General DNA / RNA KIT (HWTS-3017-50, HWS-3017-50, HWS-3017-48, i-HWS-3017-48, ne-Macro & Micro-Test Aptoutic acid extractor ( I-HWTS-3006C, HWTS-3006B) nguJiangsu Macro & Micro-Test I-Med-Tech Co, Ltd., ukukhishwa kufanele kwenziwe ngokwemiyalo ngokuqinile. IVolumu Ye-Expeated Enconyiwe ingu-80μL.
Inketho 3.
I-realent enconyiwe I-realent: Ukukhishwa kwe-nucleic acid noma i-shitinication kit (yppp15) nguTiangen Biotech (Beijing) Co, Ltd., Ukukhishwa kufanele kwenziwe ngokwemiyalo ngokuqinile. IVolumu Ye-Expeated Enconyiwe ingu-100μL.