Izinhlobo eziyi-14 ze-HPV Nucleic Acid Typing

Izinhlobo ezingu-14 ze-HPV Nucleic Acid Typing Isithombe Esifakiwe

Incazelo emfushane:

I-Human Papillomavirus (HPV) ingeyomndeni we-Papillomaviridae wegciwane le-DNA eliyindilinga elinemigqa emibili, elingamboziwe, elinobude be-genome obungaba yizibili eziyisisekelo (bp) ezingu-8000. I-HPV ithelela abantu ngokuxhumana okuqondile noma okungaqondile nezinto ezingcolisiwe noma ukudluliselwa ngokocansi. Leli gciwane aligcini nje ngokuba ngelakhe, kodwa futhi liqondene nezicubu, futhi lingathelela kuphela isikhumba somuntu kanye namaseli e-epithelial e-mucosal, okubangela izinhlobo ezahlukene zama-papillomas noma ama-warts esikhumbeni somuntu kanye nomonakalo owandayo ku-epithelium yomgudu wokuzala.

Le khithi ifanelekela ukutholwa kokuthayipha kwe-in vitro kwezinhlobo ezingu-14 zama-human papillomavirus (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68) ama-nucleic acid kumasampula omchamo womuntu, amasampula e-cervical swab yabesifazane, kanye namasampula e-vaginal swab yabesifazane. Ingahlinzeka kuphela ngezindlela ezisizayo zokuxilongwa nokwelashwa kokutheleleka kwe-HPV.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

Izinhlobo ze-HWTS-CC012A-14 ze-HPV Nucleic Acid Typing Detection Kit (i-Fluorescence PCR)

I-HWTS-CC021-Yomiswe Ngokuqandisa Izinhlobo eziyi-14 ze-Human Papillomavirus Nucleic Acid Typing Kit (Fluorescence PCR)

Isitifiketi

I-Epidemiology

Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu endleleni yokuzala yabesifazane. Izifundo zikhombisile ukuthi ukutheleleka okuqhubekayo kanye nokutheleleka okuningi kwe-human papillomavirus kungenye yezimbangela ezibalulekile zomdlavuza womlomo wesibeletho. Njengamanje, kusenokuntuleka kwezindlela zokwelapha ezisebenzayo eziqashelwayo ze-HPV. Ngakho-ke, ukutholakala kusenesikhathi kanye nokuvimbela kusenesikhathi kwe-HPV yomlomo wesibeletho kuyisihluthulelo sokuvimbela umdlavuza. Ukusungulwa kwendlela yokuxilonga izifo elula, eqondile futhi esheshayo kubaluleke kakhulu ekuxilongweni komdlavuza womlomo wesibeletho emtholampilo.

Isiteshi

I-FAM	I-HPV16, 58, ireferensi yangaphakathi
I-VIC(HEX)	I-HPV18, 33, 51, 59
CY5	I-HPV35, 45, 56, 68
I-ROX	I-HPV31, 39, 52, 66

Amapharamitha Obuchwepheshe

Isitoreji	≤-18℃ Ebumnyameni
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Umchamo, i-swab yomlomo wesibeletho, i-swab yesitho sangasese sowesifazane
Ct	≤28
CV	<5.0%
I-LoD	Amakhophi angu-300/mL
Izinsimbi Ezisebenzayo	Ingafanelana namathuluzi e-PCR e-fluorescent ajwayelekile emakethe.Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time I-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 I-LightCycler^®Uhlelo lwe-PCR lwesikhathi sangempela lwe-480 Uhlelo Lokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler