Izinhlobo eziyi-17 ze-HPV (16/18/6/11/44 TYPING)
Igama lomkhiqizo
Izinhlobo ze-HWTS-CC015 17 ze-Human Papillomavirus (16/18/6/1111/44 Typing) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza wesibeletho ungenye yezimpawu ezivame kakhulu eziphathwayo kwipheshana lokuzala labesifazane. Kukhonjisiwe ukuthi ukutheleleka okuphikelelayo kwe-HPV kanye nezifo eziningi kungenye yezimbangela ezinkulu zomdlavuza wesibeletho. Njengamanje kusesekhona ukwelashwa okusebenzayo okwamukelwa ngokuvamile komdlavuza wesibeletho obangelwa yi-HPV. Ngakho-ke, ukutholwa kusenesikhathi nokuvimbela ukutheleleka kwesibeletho okubangelwa yi-HPV yizinkinobho zokuvimbela umdlavuza wesibeletho. Ukusungulwa kokuhlolwa okulula, okuqondile futhi okusheshayo kwamagciwane kubaluleke kakhulu ekuhlolweni komtholampilo komdlavuza womlomo wesibeletho.
Insungubezi
| PCR-MIX1 | -Yenza | 18 |
| VIC / Hex | 16 | |
| Ivupho | 31,3,35,39,45,41,52,52,58,58,68,68,69,69 | |
| Cy5 | Ukulawulwa Kwangaphakathi |
| PCR-MIX2 | -Yenza | 6 |
| VIC / Hex | 11 | |
| Ivupho | 44 | |
| Cy5 | Ukulawulwa Kwangaphakathi |
Amapharamitha Ezobuchwepheshe
| Ukubeka | -18 ℃ |
| Ishalofu-impilo | Izinyanga eziyi-12 |
| Uhlobo lwe-Specimen | Isampula yomchamo, isampula yesibeletho sowesifazane, isampula yesifazane sowesifazane |
| Ct | ≤28 |
| Indlu edayisa insimbi | 300copies / ml |
| Ukucacisa | Akukho okuphambana nokusebenzisa imali nge-ureaplasma urealyticum, i-chlamydia trachomatis yepheshana lokuzala, ama-albican albican, i-neischomonas vaninalinal, i-trichomonas vaginalis, isikhunta, i-gernerla nezinye izinhlobo ze-HPV ezingambozwanga yikhithi. |
| Izinsimbi ezisebenzayo | Kusetshenziswe ama-biosystems 7500 real-time PCREMS Kusetshenziswe ama-biosystem 7500 ngokushesha amasistimu we-real-time PCR I-QuantStudio®Amasistimu we-PCR e-Real-time real SLAN-96P real-time PCRS Systems I-Lightcycler®480 izinhlelo ze-PCR zangempela zesikhathi sangempela I-LineGene 9600 Plus Real-Time Pcc Retection Systems I-MA-6000 I-CYRLE YOKUQHAWULA YOKUQALA I-Biorad CFX96 Uhlelo lwe-Real-Time PCR Biorad cfx opus 96 uhlelo lwe-real-time PCR |
Ukugeleza komsebenzi
I-reaglent enconyiwe ye-realent: I-Macro & Micro-Test Viral DNA / RNA KIT (HWTS-3017-50, HWTS-3017-50, HWS-3017-48, HWS-3017-48, HWS-3017-46, HWS-3017-46, I-HWS-3017-96, (engasetshenziswa ngeMacro & Micro-Test I-automatic nucleic acid extractor (HWTS-3006C, HWS-3006B)) nguJiangsu I-Macro & Micro-Test Med-Tech Co, Ltd. engeza i-200μL ye-saline ejwayelekile yokuvuselela i-pellet ku-STEP 2.1, bese kuthi ukukhishwa kufanele kwenziwe ngokuya ngemiyalo yokusebenzisa le mpendulo ye-extraction. IVolumu Ye-Expeated Enconyiwe ingu-80μL.
I-realent enconyiwe ye-realent: Qiamp DNA Mini Kit (51304) noma i-Macro & Micro-Test Viral DNA / RNALM COLLM (HWTS-3020-50). Faka i-200μL ye-saline ejwayelekile yokuvuselela i-pellet in Step 2.1, bese kuthi ukukhishwa kufanele kwenziwe ngokuya ngemiyalo yokusebenzisa le mpendulo ye-extraction. Ivolumu yesampula ekhishwe amasampula yonke i-200μL, futhi ivolumu yokuqedwa enconyiwe ingu-100μL.
I-reagent enconyelwe i-realent: I-Macro & Micro-3005-46-8i, HWTS-3005-8J, HWS-3005-8K, HWS-3005-8l). Faka i-200μL yesampula yokukhishwa kwesampula yokukhipha kabusha i-realet yokuvuselela i-pellet in Step 2.1, bese kuthi ukukhishwa kufanele kwenziwe ngokuya ngemiyalo yokusebenzisa le mpendulo ye-extraction.
