Izinhlobo eziyi-17 ze-HPV (16/18/6/11/44 Ukuthayipha)

Incazelo emfushane:

Leli khithi lifanelekela ukutholwa kwezinhlobo ezingu-17 ze-human papillomavirus (HPV) (HPV 6, 11, 16, 18, 31, 33, 35, 39, 44, 45, 51, 52.56, 58, 59, 66, 68) izingcezu ezithile ze-nucleic acid kusampula yomchamo, isampula ye-cervical swab yowesifazane kanye nesampula ye-vaginal swab yowesifazane, kanye ne-HPV 16/18/6/11/44 yokuthayipha ukusiza ekuxilongeni nasekuphatheni ukutheleleka kwe-HPV.

Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Igama lomkhiqizo

I-HWTS-CC015 Izinhlobo eziyi-17 ze-Human Papillomavirus (16/18/6/11/44 Ukuthayipha) Ikhithi Yokuthola I-Nucleic Acid (i-Fluorescence PCR)

I-Epidemiology

Umdlavuza womlomo wesibeletho ungenye yezimila eziyingozi ezivame kakhulu endleleni yokuzala yabesifazane. Kuye kwabonakala ukuthi ukutheleleka kwe-HPV okuqhubekayo kanye nokutheleleka okuningi kungenye yezimbangela ezinkulu zomdlavuza womlomo wesibeletho. Okwamanje kusenokuntuleka kwezindlela zokwelapha ezisebenzayo ezamukelwa kabanzi zomdlavuza womlomo wesibeletho obangelwa yi-HPV. Ngakho-ke, ukutholakala kusenesikhathi kanye nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa yi-HPV kuyizihluthulelo zokuvimbela umdlavuza womlomo wesibeletho. Ukusungulwa kokuhlolwa okulula, okuqondile nokusheshayo kokuxilongwa kwamagciwane kubaluleke kakhulu ekuxilongweni komdlavuza womlomo wesibeletho emtholampilo.

Isiteshi

I-PCR-Mix1	I-FAM	18
	I-VIC/HEX	16
	I-ROX	31,33,35,39,45,51,52,56,58,59,66,68
	CY5	Ukulawula kwangaphakathi

I-PCR-Mix2	I-FAM	6
	I-VIC/HEX	11
	I-ROX	44
	CY5	Ukulawula kwangaphakathi

Amapharamitha Obuchwepheshe

Isitoreji	-18℃
Isikhathi sokuphelelwa yisikhathi	Izinyanga ezingu-12
Uhlobo Lwesifanekiso	Isampula yomchamo, isampula yeswabhu yomlomo wesibeletho yowesifazane, isampula yeswabhu yowesifazane yesitho sangasese sowesifazane
Ct	≤28
I-LoD	Amakhophi angu-300/mL
Ukucaciswa	Akukho ukuhlangana kwe-Ureaplasma urealyticum, i-Chlamydia trachomatis yendlela yokuzala, i-Candida albicans, i-Neisseria gonorrhoeae, i-Trichomonas vaginalis, i-Mold, i-Gardnerella nezinye izinhlobo ze-HPV ezingamboziwe yikhithi.
Izinsimbi Ezisebenzayo	Izinhlelo ze-PCR ze-Applied Biosystems 7500 Real-Time Izinhlelo ze-PCR ze-Applied Biosystems 7500 Fast Real-Time I-QuantStudio^®Izinhlelo ze-PCR zesikhathi sangempela ezi-5 Izinhlelo ze-PCR Zesikhathi Sangempela ze-SLAN-96P I-LightCycler^®Izinhlelo ze-PCR zesikhathi sangempela ezingu-480 Izinhlelo Zokuthola i-LineGene 9600 Plus Real-Time PCR I-MA-6000 Real-Time Quantitative Thermal Cycler Uhlelo lwe-BioRad CFX96 Real-Time PCR Uhlelo lwe-BioRad CFX Opus 96 lwe-Real-Time PCR

Ukugeleza Komsebenzi

I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-200μL we-saline evamile ukuze uphinde umise i-pellet esinyathelweni 2.1, bese ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha. Ivolumu yokususa enconyiwe ingu-80μL.

I-reagent yokukhipha enconyiwe: I-QIAamp DNA Mini Kit (51304) noma i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Engeza u-200μL we-saline evamile ukuze uphinde umise i-pellet esinyathelweni 2.1, bese ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha. Ivolumu yesampula ekhishwe yamasampula ingu-200μL, kanti ivolumu yokususa enconyiwe ingu-100μL.

I-reagent yokukhipha enconyiwe: I-Macro & Micro-Test Sample Release Release Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Engeza u-200μL we-reagent yokukhipha isampula ukuze uphinde umise i-pellet esinyathelweni 2.1, bese ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusebenzisa le reagent yokukhipha.

Okwedlule: I-Borrelia Burgdorferi Nucleic Acid

Olandelayo: I-Mycobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin(RIF)，Resistance(INH)

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