Izinhlobo eziyi-17 ze-HPV (16/18/6/11/44 Ukuthayipha)
Igama lomkhiqizo
HWTS-CC015 17 Izinhlobo ze-Human Papillomavirus (16/18/6/11/44 Typing) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungesinye sezimila eziyingozi ezivame kakhulu emgudwini wokuzala wabesifazane. Kukhonjisiwe ukuthi ukutheleleka nge-HPV okungapheli kanye nezifo eziningi kungenye yezimbangela ezinkulu zomdlavuza womlomo wesibeletho. Okwamanje kusenokuntuleka kwemithi yokwelapha esebenzayo eyamukelekayo yomdlavuza womlomo wesibeletho obangelwa i-HPV. Ngakho-ke, ukutholakala kusenesikhathi kanye nokuvimbela ukutheleleka komlomo wesibeletho okubangelwa i-HPV yizihluthulelo zokuvimbela umdlavuza womlomo wesibeletho. Ukusungulwa kwezivivinyo ezilula, eziqondile nezisheshayo zokuxilonga amagciwane kubaluleke kakhulu ekuxilongweni komtholampilo komdlavuza womlomo wesibeletho.
Isiteshi
| I-PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| I-ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Ukulawula kwangaphakathi |
| I-PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| I-ROX | 44 | |
| CY5 | Ukulawula kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | -18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo Lwesifanekiso | Isampula yomchamo, isampula ye-swab yomlomo wesibeletho yowesifazane, isampula ye-swab yesitho sangasese sowesifazane |
| Ct | ≤28 |
| LoD | 300Amakhophi/mL |
| Ukucaciswa | Akukho ukuphinda kusebenze nge-Ureaplasma urealyticum, i-Chlamydia trachomatis yepheshana lokuzala, i-Candida albicans, i-Neisseria gonorrhoeae, i-Trichomonas vaginalis, isikhunta, i-Gardnerella nezinye izinhlobo ze-HPV ezingavalwanga yikhithi. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Izinhlelo ze-PCR Zesikhathi Sangempela SLAN-96P Real-Time PCR Systems I-LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid (006W), Extramatic Nucleic Acid HWTS-3006B)) ka-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Engeza u-200μL we-saline evamile ukuze umise kabusha i-pellet esinyathelweni sesi-2.1, bese ukukhishwa kufanele kwenziwe ngokuya ngemiyalelo yokusetshenziswa kwalesi senzo sokukhipha. Ivolumu ye-elution enconyiwe ngu-80μL.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-QIAamp DNA Mini Kit (51304) noma i-Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Engeza u-200μL we-saline evamile ukuze umise kabusha i-pellet esinyathelweni sesi-2.1, bese ukukhishwa kufanele kwenziwe ngokuvumelana nemiyalelo yokusetshenziswa kwalesi sici sokukhipha. Ivolumu yesampula ekhishiwe yamasampuli wonke angu-200μL, futhi ivolumu ye-elution enconyiwe ingu-100μL.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Engeza u-200μL we-reagent yokukhulula isampula ukuze umise kabusha i-pellet esinyathelweni sesi-2.1, bese ukukhishwa kufanele kwenziwe ngokuya ngemiyalelo yokusebenzisa lesi sici sokukhipha.
